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91.
Cr(Ⅵ)染毒对小鼠肝脏细胞凋亡以及凋亡相关蛋白的影响 总被引:1,自引:0,他引:1
为研究六价铬(Cr(Ⅵ))对生物体的细胞凋亡以及凋亡相关蛋白的改变,用0、25、50和100 mg·kg-1的重铬酸钾(K2Cr2O7)对小鼠进行1d或持续5 d灌胃,检测肝脏细胞凋亡以及p53、Bcl-2、Bax蛋白表达水平和caspase-3酶的激活.实验结果显示,Cr(Ⅵ)染毒1 d或5 d后,小鼠肝细胞凋亡率随染毒剂量的增加而增加,并呈明显的剂量-反应关系;p53、Bax蛋白表达升高,Bcl-2蛋白表达下降,easpase-3酶激活增加,且p53、Bcl-2蛋白表达水平和caspase-3酶激活水平在100mg·kg-1剂量组的2个染毒时间段与对照组相比均具有显著性差异,Bax蛋白表达水平仅在100 mg·kg-1染毒5 d的实验组与对照组相比具有显著性差异.结果表明,Cr(Ⅵ)能诱导小鼠肝脏细胞凋亡,p53、Bcl-2、Bax和caspase-3在这个过程中可能起重要作用. 相似文献
92.
高效液相色谱法测定水和废水中邻(对)硝基氯苯 总被引:5,自引:0,他引:5
用高效液相色谱法测定了水和废水中的邻 (对 )硝基氯苯。流动相为甲醇 /水 (70 /3 0 ) ,紫外检测波长 2 2 0 nm,方法的线性范围为 0~ 1 0 0 μg/ml,相关系数大于 0 .9998,检测限为 2 μg/L。本方法快速、准确 ,满足地面水的测定要求 相似文献
93.
应用核酸酶P1促进的32P-后标记技术对苯醌和氢醌处理人血淋巴细胞后形成的DNA加合物进行了研究。结果表明,人血淋巴细胞被苯醌和氢醌处理24h之后,均形成了同一种DNA加合物。两种化合物在不同浓度下处理人血淋巴细胞每107核苷酸中有0.01~10个核苷酸形成加合物。为了达到同等DNA加合物水平,氢醌比苯醌需要更高的浓度。小牛胸腺DNA与苯醌、氢醌反应可生成5种DNA加合物。用苯醌或氢醌处理淋巴细胞所形成的加合物种类与上述纯DNA与苯醌,氢醌形成的5种加合物在双向薄层层折图谱上完全不相符合。这些结果意味着苯醌、氢醌与DNA的加合作用在细胞内存在着修饰机制。 相似文献
94.
Two cases of amniotic fluid cell cultures are reported, where a familial, non-heterochromatic 9p+ variant has been found. 相似文献
95.
96.
Hypospadias is one of the most prominent and characteristic midline defects in male infants with the Wolf-Hirschhorn (4p —) syndrome. In this report we present a case in which hypospadias was identified prenatally at 29 weeks' gestation in association with intrauterine growth retardation. Cytogenetic evaluation after birth confirmed a 46, XY, del(4)(p14) karyotype. The prenatal identification of hypospadias in fetuses with intrauterine growth retardation and normal amniotic fluid should suggest a diagnosis of Wolf-Hirschhorn syndrome. 相似文献
97.
We report the prenatal diagnosis of Wolf-Hirschhorn syndrome (4p –) in a 24-week-old fetus. Echographic features included cystic hygroma, a complex heart defect with right ventricular hypoplasia, and a large placental chorioangioma. We suggest that chorioangioma may be associated with chromosomal imbalance and that systematic careful morphologic examination of the fetus and karyotyping of any pregnancy in which large chorioangioma is detected is advisable. Jugular lymphatic obstruction sequence has not been reported so far in association with 4p– syndrome. 相似文献
98.
L. Shivashankar E. Whitney G. Colmorgen T. Young G. Munshi D. Wilmoth K. Byrne G. Reeves Dr. D. S. Borgaonkar S. R. Picciano P. A. Marttn-Deleon 《黑龙江环境通报》1988,8(2):85-91
A case of tetrasomy i(12p) detected prenatally is reported. The patient, a black, 33-year-old G3P2002 at 24 weeks' gestation with an unremarkable family history presented herself for prenatal care. Ultrasound examination showed a fetus with diminished femoral and humeral lengths, and hydramnios. A level II scan confirmed the presence of an omphalocele. Amniocentesis at 31 weeks showed 47,XY, + i(12p) karyotype. An infant with multiple congenital anomalies was delivered at 34 weeks. The infant died after 5 h. Genetic and ultrasonographic examinations in the third trimester were helpful in the investigation of this fetus with multiple congenital anomalies. The careful, complete team counselling afforded by this approach enabled the mother and family to be well adjusted to the strong possibility (and subsequent reality) of an abnormal infant. 相似文献
99.
Veska Toncheva An Van Den Bulcke Etienne Schacht Joris Mergaert Jean Swings 《Journal of Polymers and the Environment》1996,4(2):71-83
Poly (-caprolactone) (PCL), poly (-valerolactone) (PVL), poly (-caprolactone-co--valerolactone) [P(CL-co-VL)], and poly (-caprolactone-co-ethylene oxide-co--caprolactone) (PCL-PEO-PCL) were synthesized by ring-opening and diol-initiated polymerization of -caprolactone and -valerolactone. The degradation of the samples by chemical hydrolysis and in a soil burial test was evaluated. It was found that PCL, PVL, and P(CL-co-VL) degrade mainly enzymatically. The rate of degradation depends on their molecular weight, chemical structure, composition, and morphology. PCL-PEO-PCL block copolymers exhibit a repelling effect to the microorganisms in the soil, which depends on the molecular weight and relative amount of PEO block in the copolymer. 相似文献
100.
A family is described in which two anencephalic fetuses were identified in two pregnancies. Autopsy revealed kidney anomalies in both fetuses. Chromosome analysis was performed only on the second fetus, which had a 46,XY,lOq+ karyotype. Parental chromosome analysis showed the maternal karyotype to be 46,XX,t(2;10) (p24;q26) thus demonstrating that the fetus was carrying a duplication 2(p24→pter). Recurrence risks for anencephaly based on the cytogenetic abnormality were much higher than those which would be quoted for isolated anencephaly. This points out the necessity for complete diagnostic studies when a fetus with a neural tube defect is identified. The literature in regard to the 2p duplication phenotype is reviewed. It is possible that the duplication of the distal segment of 2p results in a neural tube defect/kidney anomaly phenotype. 相似文献