电絮凝处理油田生化出水

为了减轻对后续处理中超滤膜的污染,采用电絮凝法处理油田生化出水,降低油田生化出水中的有机物含量。研究了电流强度、曝气时间和pH值对水中总有机碳（TOC）和浊度除去效果的影响以及pH值随曝气时间的变化趋势,通过红外光谱对絮凝处理前后水中总溶解固体进行了分析,优化了电絮凝的工艺条件。研究表明,当水流速度控制在50 mL/s,电流强度为2 A,500 mL絮凝出水的曝气时间为30 min时,整套絮凝工艺对TOC的去除率为48%,浊度去除率为42.9%,COD去除率为44%。     

UASB-FEO-氨吹脱-CASS工艺在垃圾渗滤液处理中的应用

针对垃圾渗滤液可生化性差、氨氮含量高等特点和国内处理工艺现状,采用UASB-FEO-氨吹脱-CASS工艺处理合肥市龙泉山城市垃圾填埋场垃圾渗滤液。介绍了工艺的重要构筑物及其设计参数。运行实践表明,整套装置运行平稳,出水水质满足设计要求。根据出水水质对该工艺进行技术分析以及运行结果的讨论,并与新发布的技术规范及排放标准进行对比分析。     

生物强化处理石油污染土壤理化性质和微生物学特性的纵向分布特征

采用原位强化生物修复技术对某区块石油污染土壤进行为期16个月的生物修复,考察了处置后污染土壤理化性质、微生物学特性以及石油烃组成的纵向分布特征。实验结果表明,经过修复后各土层的石油烃去除率是表层土IN-3(50.42%)中层土IN-2(23.54%)底层土IN-1(10.51%);IN-1处于缺氧环境,存在硫酸盐还原和反硝化作用,使得土壤pH值从7.86±0.03降低至7.27±0.03,土壤总氮从2.53±0.13 g/kg降低至0.77±0.04 g/kg;厌氧菌的种群数量是IN-1(10.43±0.71×104CFU/g)IN-3(6.74±0.39×104CFU/g)IN-2(5.15±0.42×104CFU/g),放线菌数量与石油烃含量显著负相关(r=-0.989,p=0.0110.05);IN-3对饱和份和芳香份的降解率最高,分别达到了70.27%和54.52%,远高于IN-2和IN-1;模拟蒸馏结果表明,IN-3正构烷烃得到了很大程度的去除,缺氧的IN-1对正构烷烃去除得较少;厌氧菌数量与胶质和沥青质去除率之间成正相关关系,对于污染源较为分散的污染区域,采用原位生物强化修复时可以考虑引入厌氧修复。     

Toxicological characterization of the landfill leachate prior/after chemical and electrochemical treatment: A study on human and plant cells

In this research, toxicological safety of two newly developed methods for the treatment of landfill leachate from the Piškornica (Croatia) sanitary landfill was investigated. Chemical treatment procedure combined chemical precipitation with CaO followed by coagulation with ferric chloride and final adsorption by clinoptilolite. Electrochemical treatment approach included pretreatment with ozone followed by electrooxidation/electrocoagulation and final polishing by microwave irradiation. Cell viability of untreated/treated landfill leachate was examined using fluorescence microscopy. Cytotoxic effect of the original leachate was obtained for both exposure periods (4 and 24 h) while treated samples showed no cytotoxic effect even after prolonged exposure time. The potential DNA damage of the untreated/treated landfill leachate was evaluated by the comet assay and cytokinesis-block micronucleus (CBMN) assay using either human or plant cells. The original leachate exhibited significantly higher comet assay parameters compared to negative control after 24 h exposure. On the contrary, there was no significant difference between negative control and chemically/electrochemically treated leachate for any of the parameters tested. There was also no significant increase in either CBMN assay parameter compared to the negative control following the exposure of the lymphocytes to the chemically or electrochemically treated landfill leachate for both exposure periods while the original sample showed significantly higher number of micronuclei, nucleoplasmic bridges and nuclear buds for both exposure times. Results suggest that both methods are suitable for the treatment of such complex waste effluent due to high removal efficiency of all measured parameters and toxicological safety of the treated effluent.     

Hematology and Some Biochemical Parameters of Wild Rodents in Pistachio Gardens of Kerman Province, Southeast Iran

Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province, Southeast Iran from 2007 to 2009, then some physiological parameters of them were measured. The samples were identified as follow: Nesokia indica, Meriones persicus, Meriones lybicus and Tatera indica. Blood samples were obtained from the heart, then the blood parameters (glucose, cholesterol, triglyceride, total protein, HDL, red and white blood cell number) in wild species of rodents and laboratory rat were compared. The results showed that there were no significant differences in serum glucose, triglyceride, HDL and total protein levels among different experimental groups. The concentration of cholesterol in T. indica was more than that in N. indica (P < 0.01). The total numbers of red blood cells also showed significant difference between wild garden rodent species and laboratory rat (P < 0.01), while the numbers of white blood cells showed no significant difference.     

生化法处理含氨废水动力学研究

对生化法处理含氨废水过去中有重要影响的动力学因素进行了试验研究和分析，得出了利用高效微生物菌群废水中COD和NH3－N的动力学条件，温度为20－35℃，PH为7－8，DO大于2.6mg/L,MLSS大于2.6g/L，C／N小于3。在此条件下处理含氨废水，处理后的出水达到国家化肥行业一级排放标准。     

孤岛油区外排采油污水生化处理技术的研究

针对孤岛污水矿化度高,Cl-含量高,油含量和COD含量严重超标的现象,采取微生物接触氧化工艺进行外排采油污水治理的工艺研究。在处理过程中首先对菌种进行了严格的筛选和培养,然后在室内进行了模拟试验,最后在现场进行了中试。目前已根据中试结果,对孤岛污水原有的污水池进行了改造,设置了隔油池、气浮池、接触氧化池和沉淀池四个部分,经过一年的运行,成功地实现了污水的达标排放。     

瓦勃氏呼吸仪测定乐果合成废水的可生化性

本文概述了瓦勃氏呼吸仪测定有机污染物的可生化性的基本原理和方法。通过测定用乐果合成废水驯化后的微生物的生化呼吸线和相对耗氧速度曲线,结果表明:乐果合成废水是有毒的,但是完全可以被特异驯化后的微生物所降解;其降解速度与时间和废水所含污染物的浓度有关。     

DESK TOP METHODOLOGY FOR MODELING BIOCHEMICAL OXYGEN DEMAND IN STREAMS1

: The modeling of dissolved oxygen in streams is a widely used technique, upon which a great deal of money has been spent. This paper concludes that the standard methods of DO modeling by computer are unnecessarily complex, and that for some purposes, they can be replaced without loss of accuracy by desk top BOD models. Taking as an example, a set of data used in DO modeling, it is shown (a) that the data are grossly inconsistent, (b) that simultaneous gathering of data introduces errors in streams of long travel time, (c) that much more data as to pollutant concentrations should have been obtained, and (d) that 24-hour DO data could have been dispensed with.     

Spore inhibition-based enzyme substrate assay for monitoring of aflatoxin M1 in milk

A spore germination-based concept and its transformation into a field level prototype for monitoring aflatoxin M₁ (AFM₁) in milk was developed. Initially, 15 strains of Bacillus spp. procured from different culture collection were screened for AFM₁ sensitivity using spot assay and marker strain showing inhibition at 0.5 ppb was selected based upon maximum zone of inhibition. The selected strain B. megaterium 2949 was further screened for different enzymes activities and subsequently its spores were produced to an extent of 73.13% ± 3.197% in newly developed sporulation medium containing beef extract (0.0075% ± 0.0004%), yeast extract (0.015% ± 0.001%), peptone (0.0375% ± 0.0016%), and sodium chloride (0.0375% ± 0.0018%). A spore germination-based concept/ assay was optimized by immobilizing spores in eppendorf with pretreated milk (80°C/15 min) containing germinant and chromogenic substrate followed by incubation at 37°C. The appearance of sky blue color within real time of 45 min indicated spores germination and release of specific marker enzyme such as acetyl esterase and its specific action on chromogenic substrate which demonstrates absence of AFM₁ in milk. However, if there was no color change, presence of AFM₁ at 0.5 ppb MRL was denoted by Codex. The developed concept on AFM₁ detection was validated and a correlation of 0.97 was established with AOAC approved Charm 6602 and ELISA at Codex MRL with minimal false positive and negative results. The cost effective test has potential application in dairy farms, manufacturing, and R&D units for routine monitoring of AFM₁ in milk.