Reduction of sera requirements in amniotic fluid cell culture

Reduction in serum requirement for culture of primary human amniotic fluid cells can be achieved by the addition of 10 growth-promoting factors to the nutrient medium. This supplemented medium preserves cell types normally found in amniotic fluid cell cultures supplemented with 20–30 per cent fetal bovine serum. The volume of amniotic fluid required to initiate culture can be as little as 1 ml. Amniotic fluid samples contaminated with red blood cells with no visible clot also grow well in the low serum medium. Cell-free amniotic fluid combined with equal parts of supplemented medium is useful in initiating cell culture.     

The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cellsin vitro

The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm² plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O₂) instead of conventional air (18 per cent O₂). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O₂. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure.     

固定化酵母菌对活性艳蓝KN-R的脱色研究

采用海藻酸钙加活性碳的方法对酵母菌进行固定化。正交试验结果表明，三者合适配比为海藻酸钠3％，活性碳10％，氯化钙4％。此方法制得的凝胶颗粒机械强度好、经久耐用。固定化酵母菌凝胶小球最适条件为：培养时间为48h，培养温度为37℃，培养基pH为3．0，凝胶量为10g。固定化细胞经多次脱色后能重复使用，4次脱色后其脱色率还达79．2％。     

Effect of Cross-Linking Waste Protein with Dialdehydes on Its Biodegradation Under Anaerobic Conditions

Most native polymers used in processing and application technologies are admittedly disposable from the environment in a biologic manner, but products possess low mechanical strength. One of the paths to increasing this attribute (if feasible) is their cross-linking, which may, however, affect their readiness to biodegradation. In the presented work this condition was observed on the example of waste protein (Hykol B) cross-linking by means of glutardialdehyde and glyoxal. Degree and course of cross-linking were determined through impedance spectroscopy. The objective of this work also was to obtain data for constructing a sensor capable of following the cross-linking course in real time, for potential industrial application of Hykol in continuous production. Impedance spectroscopy proved to be applicable even to this kind of material marked by considerable water content and exhibiting relatively high electric conductivity; so far it had been used only for materials of low conductivity. An aqueous environment inoculated with digested anaerobic sludge from a municipal wastewater treatment plant was selected for modeling anaerobic conditions. The relation was studied between cross-linking degree given by content of cross-linking agent (determined by impedance spectroscopy) and biodegradation degree under anaerobic conditions. It was confirmed that network density as given by quantity of added agent not only reduced breakdown degree but also slowed the course of the process. This fact is particularly obvious with cross-linking by means of glyoxal; network density is thus dependent on type of employed substance, which affect type and structure of created network. That not merely forms an obstacle during polymer swelling and dissolution but also prevents access of bacteria to source of metabolized organic carbon.     

深水淹没高喷注浆帷幕技术

介绍导孔钻进、旋喷注浆成桩、桩顶沉降回灌等三重管高压旋喷注浆技术、参数、设备、工艺及其在上海市杨树浦水厂改建工程中的成功应用。该技术对保证安全、优质、高效的施工发挥了重要作用。     

重力挡土墙地震反应研究评述

首先，对重力挡土墙的地震反应机理进行了探讨，在此基础上简要回顾了国内外抗震设计规范的演变进程；然后，对挡土墙地震反应的研究方法进行了细致评述，包括拟静力极限平衡分析法、简化条件解析法、极限位移法、地震土压力简化公式、集总参数法、整体有限元法、现场地震调查、离心机试验、振动台试验和原型观测实验等；最后，指出了需要解决的问题并提出了今后的研究方向。     

溜砂坡的成灾机理与防治对策

溜砂坡足我国西部山区公路的主要地质灾害之一。由于对溜砂坡的形成发展机理认识不足，目前采用的治理措施不能很好地解决溜砂灾害，有些工程措施反而会加大灾害程度。通过对川藏公路沿线溜砂坡的调查研究，阐述了溜砂坡的最基本特性是其生长性，分析了溜砂坡的成灾机理和危害性特点，有针对性地提出了符合西部地区经济条件的溜砂坡防治措施。     

A Parallel Study of SAR Levels in Head Tissues for Three Antennas Used in Cellular Telephones: Monopole, Helix and Patch

Summary This paper is a comparative study of several antennas commonly used in cellular telephones. These include a monopole, a helix-monopole and a patch antenna. Each one of these structures is modeled and numerically tested using finite-difference time-domain simulation and human models based on magnetic-resonance images, which allow for inclusion of details of the human body in the simulation. The testing procedure involves antenna simulation in the proximity of the human head. The behavior of each antenna is evaluated for variable distances from the head geometry (0, 1, 2, 4, 8 and 16 mm). Continuous waveform, representative of the sources used in mobile telephones, (250 mW, 1.8 GHz) is used as the form of the antenna excitation. The simulation outputs used as measures for this comparative study include transmitting and receiving antenna characteristics and the specific absorption rate (SAR). The SAR levels for the head tissues are calculated for and with accordance to the two currently accepted standards: Federal Communications Commission (FCC) and International Commission on Non-Ionizing Radiation Protection (ICNIRP). The computed SAR levels within each of the considered tissues vary for the three antennas under investigation and are within the determined health safety standards. Results suggest that the patch antenna may be the structure of choice when considering safety standards, as its radiation yields the lowest local SAR in the head tissues.     

Performance evaluation of reverse logistics enterprise – an agent-based simulation approach

Reverse logistics (RL) has been applied in many industries and sectors since its conception. Unlike forward logistics, retracing consumer goods from the point of consumption to the point of inception is not a well-studied process. It involves many uncertainties such as time, quality and quantity of returns. The returned products can be remanufactured, have parts harvested, or be disposed safely. It is important to implement these activities in a cost-effective manner. The aim of this research is to measure the performance of the RL enterprise with the help of an agent-based simulation model. The major entities in the RL network are considered as Agents that can act independently. There are several different agents: collector agent, sorting-cum-reuse agent, remanufacturing agent, recycler agent, supplier agent and distributor agent. The individual performances of the agents are measured and recommendations are given to improve their performance, leading to the enhancement of the total performance of the RL enterprise. The approach is applied to a case study involving cell phone remanufacturing.     

微生物燃料电池改性阳极处理PTA废水

探讨了不同改性阳极对微生物燃料电池(microbial fuel cell,MFC)产电性能及其对MFC处理难降解废水能力的影响.以单室空气阴极为基础,利用0.1 g电气石、质量分数75%二氧化锰/埃洛石纳米管(manganese bioxide/halloysite nanotube,MnO_2/HNT)和多壁碳纳米管-羧基(multi-walled carbon nanotube-carboxyl,MWCNT-COOH)对MFC阳极进行修饰.结果表明,不同改性阳极的MFC对含精对苯二甲酸(purified terephthalic acid,PTA)废水的去除率均高于70%,且化学需氧量(chemical oxygen demand,COD)去除率在79%以上.相较于其他几种改性阳极,以MWCNT-COOH改性材料作阳极的MFC产生的最大输出电压最高,获得的最大功率密度最高,分别为529 mV和252.73 mW·m~(-2).