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91.
92.
Laird Jackson 《黑龙江环境通报》2003,23(10):837-846
Although fetal cells have been known to escape to the maternal circulation for a number of years, research attempts to use them for prenatal diagnosis have not had any consistent success. This review attempts to trace the history of such attempts and to document their progress and reasons for success or failure. The opinions of recent conferences including that of the US National Institute of Child Health and Human Development, a sponsor of major US research in the field, are reported and discussed. It is concluded that although basic work has demonstrated the biologic availability of both fetal cells and their free DNA representatives in the maternal circulation at gestational ages relevant to prenatal diagnosis, much work remains to develop practical technology for their consistent recovery and assay. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
93.
EMMC process for combined removal of organics, nitrogen and an odor producing substance 总被引:2,自引:0,他引:2
In order to improve the process performance regarding the removal of organics, nitrogen, and an odor-causing compound (sulfide) contained in domestic wastewater, an entrapped-mixed-microbial cell (EMMC) with and without humic substances for both fixed and moving carrier reactors and conventional suspended growth culture (i.e. conventional activated sludge process) were investigated simultaneously. Both synthetic (simulated to the organics concentration of general domestic sewage) and actual domestic wastewater were investigated under operational conditions of 12 h of hydraulic retention time (HRT) with 1 h of aeration and 1 h of non-aeration, and 6 h of HRT with continuous aeration, at a room temperature of 25 +/- 2 degrees C. It was found that entrapping humic substances in the EMMC carriers had no impact on the removal of organics, nitrogen, and the odor-producing compound. Additionally, the performance of the EMMC moving carrier system for the removal of these pollutants is similar to that of the EMMC fixed carrier system. In general, the EMMC associated systems which provide high solids retention time achieve a better removal of chemical oxygen demand (COD), nitrogen, and the odor-producing substance than the suspended growth system for both HRTs of 6 h (continuous aeration) and 12 h (1 h of aeration and 1 h of non-aeration). Both the fixed and moving carrier EMMC processes, therefore, have the potential for improvement or replacement of the existing conventional activated sludge process with regard to improving the effluent qualities (such as COD, nitrogen and odor-producing compound) for reuse/disposal. 相似文献
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Sakai Y Shoji R Kim BS Sakoda A Suzuki M 《Environmental monitoring and assessment》2001,70(1-2):57-70
Among bioassays for evaluating various impacts of chemicalson humans and ecosystems, those based on culturedmammalian-cells can best predict acute lethal toxicity to humans. Weexpect them to be employed in the future in environmentalrisk management alongside mutagenicity tests and endocrine-disrupting activity tests. We recently developed adisposable bioassay device that immobilizes humanhepatocarcinoma cells in a small micropipette tip. Thisenables very quick (within 2 h) evaluation of acute lethaltoxicity to humans. For bioassay-based environmentalmanagement, 2 promising approaches have been demonstrated bythe US-EPA: toxicity identification evaluation (TIE) andtoxicity reduction evaluation (TRE). The Japanese Ministryof Environment has been supporting a multi-center validationproject, aimed at assembling a bioassay database. To makefull use of these resources, we present a numerical modelthat describes contribution of individual chemical toobserved toxicity. This will allow the selection of the mosteffective countermeasure to reduce the toxicity. Bioassay-based environmental risk management works retrospectively,whereas impact assessment using substance flow models andtoxicity databases works prospective. We expect that these 2approaches will exchange information, act complementarily,and work effectively in keeping our environment healthy inthe 21st century. 相似文献
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96.
潮湿巷道风流温度与湿度变化规律分析 总被引:1,自引:1,他引:1
通过对矿井风流与围岩热湿交换理论的研究,提出理论上更可靠的风流温、湿度计算方法,编制了模拟解算矿井风流与围岩热湿交换的计算机程序,解算出潮湿巷道风流温度及湿度的变化规律,并分析通风时间、湿度系数等参数对风流温度及湿度变化规律的影响;沿风流流动方向,风流温度及湿度不断增加;巷道风流温度及湿度随着通风时间的增加而不断减小,通风时间越长减小的幅度越小;围岩壁面湿度系数对风流温度及湿度的影响较大,其他参数不变时,壁面湿度系数越大,风流温度越小,风流湿度越大。 相似文献
97.
联苯代谢对微生物的生长胁迫及分裂抑制 总被引:1,自引:0,他引:1
以联苯/多氯联苯降解菌株R04(Rhodococcus sp.R04)和几种模式微生物为研究对象,利用高效液相色谱、荧光显微镜、扫描电子显微镜等分析微生物在联苯及其代谢物培养条件下细胞分裂和形态的变化.结果表明联苯及其代谢产物对红球菌R04和几种模式微生物细胞的分裂有抑制作用,并对部分微生物形态有影响.与前体-联苯及其代谢产物2-羟基-6-酮基-6-苯基-2,4-己二烯酸相比,2,3-二羟基联苯对G+、G-细菌,或是酵母细胞分裂都有较强的抑制和形态的改变.2,3-二羟基联苯导致R.R04和缺陷型R.R04细胞形成不完整隔膜的比例增加;造成96.4%的大肠杆菌BL21细胞表面凹陷,胞质内容物流失,菌体体积缩小;导致枯草芽孢杆菌89.6%的细胞体积明显缩小;导致金黄色葡萄球菌基本没有细胞能形成完整的分裂隔膜;导致红酵母细胞能进行出芽生殖的比例从64.2%降低到19.3%,但对其细胞形态无明显改变.联苯代谢物2,3-二羟基联苯对红球菌R04及其它微生物细胞分裂和增殖的抑制作用比其前体-联苯强,建议在研究环境化合物与微生物互作时,应考虑环境化合物代谢的毒性效应. 相似文献
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100.
为了计算综放工作面沿空留巷支护阻力,以结构力学理论为基础,分析综放工作面沿空留巷支护阻力计算模型,建立沿空留巷围岩和支护体的结构力学模型,应用超静定结构和静定结构分析支护体与沿空留巷围岩相互作用机制,推导出巷旁支护阻力的计算公式.结果表明,进行沿空留巷支护阻力力学分析时,要考虑顶板极限断裂前和断裂后两种状态下的受力情况,支护阻力与岩层厚度、容重、煤体极限平衡区宽度、跨距、支护体宽度和巷道宽度等因素密切相关.工业性试验表明,该技术的应用是成功而有效的.因此,综放巷内沿空留巷围岩结构力学模型和支护阻力计算公式是合理的,可在类似条件的综放工作面中应用. 相似文献