4种多环芳烃对仿刺参(Apostichopus japonicus)原肠胚发育的急性毒性研究

海洋中的多环芳烃(PAHs)具有较强的生物毒性,且海洋动物早期发育阶段是对环境因素变化响应的最敏感阶段。为探究海洋多环芳烃类有机污染物对仿刺参(Apostichopus japonicus)早期发育阶段原肠胚的毒性影响,采用半静态毒性实验方法,分别考察了4种多环芳烃苯并[a]芘、3-甲基菲、惹烯及2-甲基蒽对仿刺参原肠胚的24、48、72、96 h急性毒性效应。结果表明,在10、50、100、200μg·L~(-1)暴露浓度下,随着暴露时间的延长和暴露浓度的升高,4种多环芳烃对仿刺参原肠胚产生不同程度的急性毒性效应,仿刺参原肠胚存活率与4种多环芳烃浓度之间分别存在显著的剂量-效应关系(P0.05)。苯并[a]芘对仿刺参原肠胚在24、48 h的半致死浓度(LC_(50))分别为294.4、225.64 mg·L~(-1),3-甲基菲在24、48、72、96 h的LC_(50)分别为404.5、300.7、81.4、17.6mg·L~(-1),惹烯在24、48、72 h的LC_(50)分别为243.1、230、186 mg·L~(-1),2-甲基蒽在24、48、72、96 h的LC_(50)分别244、231.6、152.6、142.9 mg·L~(-1)。4种多环芳烃的安全浓度(SC)分别为39.76、49.8、61.8、62.6μg·L~(-1),其毒性大小顺序为苯并[a]芘3-甲基菲惹烯2-甲基蒽。基于定量构效关系(QSAR)的研究结果可知多环芳烃化合物的毒性差异可能与分子结构等性质有关。该实验为深入研究多环芳烃对海洋环境的毒性效应提供了理论依据。     

乙虫腈悬浮剂对新出房意大利蜜蜂的毒性研究

乙虫腈是在我国登记并广泛使用的氟虫腈替代药剂,其商品化制剂为拜尔公司研发的100 g·L~(-1)悬浮剂,目前关于乙虫腈制剂对非靶标生物的毒性研究少有报道。为探究乙虫腈制剂对蜜蜂的毒性作用,采用人工饲喂法分别测定了100 g·L~(-1)乙虫腈悬浮剂对新出房意大利蜜蜂的48 h急性和14 d慢性毒性作用。结果显示:乙虫腈悬浮剂对新出房意大利蜜蜂的48 hLC_(50)值为0.21 mg·L~(-1)(95%置信限0.16～0.26 mg·L~(-1)),相应的48 h-LD50为5.0×10~(-3)μg a.i.·蜂~(-1)(95%置信限1.0×10~(-3)～1.0×10~(-2)μg a.i.·蜂~(-1));在亚致死剂量下,蜜蜂死亡数量随暴露时间的延长而增多,且100 g·L~(-1)乙虫腈悬浮剂会引起蜜蜂显著的氧化应激反应,诱导抗氧化酶SOD和CAT活力、脂质过氧化产物MDA含量、解毒酶GST活力和底物GSH含量的显著变化。研究表明,乙虫腈悬浮剂对意大利蜜蜂毒性较高,即使在亚致死剂量下亦存在显著的影响。田间施用应防止药剂的漂移污染,并避免在作物花期施用;新出房意大利蜜蜂对药剂敏感,未来可探索其作为风险指示生物应用的可能。     

紫外吸收剂在湖泊中的分布及其对底栖动物的毒性效应

紫外吸收剂大量用于工业材料和个人护理品中,近年来在水体中不断被检出,同时由于其高度亲脂性,易在底泥和水生生物体内富集,产生潜在的毒性效应,已成为一类新型污染物。本文综述了紫外吸收剂在湖泊环境中的分布和生物富集效应,重点分析了二苯甲酮类等典型紫外吸收剂对底栖动物的药物代谢系统、抗氧化系统及其生长发育的影响及其毒性作用机制,并对未来该领域的研究进行了展望。     

Reflections on 40 years of Disasters, 1977–2017

This reflection, based on a keynote address to celebrate the fortieth anniversary of Disasters on 14 September 2017, begins by considering some of the prevailing assumptions that existed at the time of the journal's creation. Next is a summary of significant milestones during the past 40 years, covering major global trends, changing disaster impacts, and key developments in disaster risk management. Contrasting approaches in the first and fourth decade of the journal's history are then followed by examples of changes in terminology in the disaster field as an indication of shifting values. The paper goes on to explore the context of 2017, with observations of problem areas such as the loss of knowledge, failures to tackle the root causes of disasters, shortfalls in international assistance, and some negative attitudes. It closes by evaluating some current positive occurrences, including a reduction in casualties, sharing of experience, technological advances, and dramatic improvements in disaster warning.     

Fieldwork after conflict: contextualising the challenges of access and data quality

Despite sustained scholarly interest in post‐conflict states, there has not been a thorough review and analysis of associated methodology and the challenges of conducting research in these contexts. Addressing this gap, this paper directs attention to the particular effects of these settings on access and data quality and their ramifications for the resulting scholarship. It assesses the intrinsic challenges of performing fieldwork in these environments, drawing on both relevant social science literature and the authors’ experiences of carrying out research in Afghanistan and Timor‐Leste. The study demonstrates that the post‐conflict environment moulds research design and, consequently, influences how questions are answered as well as the questions asked. Moreover, it highlights ways to mitigate these issues. This work is of relevance to scholars planning to engage in field research and to researchers reflecting upon their work, as well as to policymakers who are considering undertaking programmes or commissioning research in post‐conflict areas.     

E-bikers’ braking behavior: Results from a naturalistic cycling study

Abstract

Objective: The number of e-bike users has increased significantly over the past few years and with it the associated safety concerns. Because e-bikes are faster than conventional bicycles and more prone to be in conflict with road users, e-bikers may need to perform avoidance maneuvers more frequently. Braking is the most common avoidance maneuver but is also a complex and critical task in emergency situations, because cyclists must reduce speed quickly without losing balance. The aim of this study is to understand the braking strategies of e-bikers in real-world traffic environments and to assess their road safety implications. This article investigates (1) how cyclists on e-bikes use front and rear brakes during routine cycling and (2) whether this behavior changes during unexpected conflicts with other road users.

Methods: Naturalistic data were collected from 6 regular bicycle riders who each rode e-bikes during a period of 2 weeks, for a total of 32.5?h of data. Braking events were identified and characterized through a combined analysis of brake pressure at each wheel, velocity, and longitudinal acceleration. Furthermore, the braking patterns obtained during unexpected events were compared with braking patterns during routine cycling.

Results: In the majority of braking events during routine cycling, cyclists used only one brake at a time, favoring one of the 2 brakes according to a personal pre-established pattern. However, the favored brake varied among cyclists: 66% favored the rear brake and 16% the front brake. Only 16% of the cyclists showed no clear preference, variously using rear brake, front brake, or combined braking (both brakes at the same time), suggesting that the selection of which brake to use depended on the characteristics of the specific scenario experienced by the cyclist rather than on a personal preference. In unexpected conflicts, generally requiring a larger deceleration, combined braking became more prevalent for most of the cyclists; still, when combined braking was not applied, cyclists continued to use the favored brake of routine cycling. Kinematic analysis revealed that, when larger decelerations were required, cyclists more frequently used combined braking instead of single braking.

Conclusions: The results provide new insights into the behavior of cyclists on e-bikes and may provide support in the development of safety measures including guidelines and best practices for optimal brake use. The results may also inform the design of braking systems intended to reduce the complexity of the braking operation.     

二氯喹啉酸对湘云鲫的急性毒性及对鱼鳃、肝脏ATP酶活性的影响

研究了二氯喹啉酸对湘云鲫的急性毒性和 (1/8～ 1/2 )LC50 浓度下对湘云鲫鳃、肝脏中Na K ATPase和Mg2 ATPase活性的影响。结果表明 :(1)在 2 3± 1℃条件下 ,二氯喹啉酸对湘云鲫的 2 4、4 8、72、96h的LC50 分别为 15 1.6 4、15 0 .92、14 9.6 0、14 9.14mg·L-1,是一种低毒性农药 ;(2 )鱼鳃和肝脏中Na K ATPase、Mg2 ATPase活性的抑制随浓度的增加而加强 ,表现出显著的浓度 -效应关系 ,是一种较好的生物指示剂。此外 ,还讨论了二氯喹啉酸抑制ATPase活性的机制     

孕激素左炔诺孕酮长期低剂量暴露对雄性斑马鱼的生殖毒性

孕激素是广泛使用的高生物活性药物,其残留广泛存在于水环境中,对水生生物造成潜在威胁。本论文研究孕激素左炔诺孕酮(LNG)长期暴露对雄性斑马鱼的生殖毒性。将斑马鱼胚胎暴露于环境相关浓度的LNG(10, 33, 100 ng·L~(-1))至性成熟(受精后142 d),得到100%的雄性斑马鱼。将得到的雄鱼与未暴露的雌鱼配对,结果发现100 ng·L~(-1) LNG处理组雄鱼使得未暴露雌鱼产卵的数量较对照组雄鱼使得未暴露雌鱼产卵的数量显著减少。33和100 ng·L~(-1)处理组雄鱼与未暴露雌鱼配对所得子代在受精后7 d内的存活率较对照组雄鱼与未暴露雌鱼配对所得子代的存活率显著降低。33 ng·L~(-1) LNG显著升高总活力、活跃精子活力、平均路径速度、直线运动速度、曲线运动速度等精子活力参数,但其他浓度的LNG并不影响精子的活力。LNG在33和100 ng·L~(-1)的浓度下显著降低雄鱼血浆中11-酮基睾酮(11-KT)的含量,且在3个浓度下均显著降低雄鱼血浆中雌二醇(E2)的含量,但对睾酮(T)无明显影响。此外,LNG暴露可干扰雄鱼大脑和精巢中孕激素受体(npr, mprα, mprβ)、雌激素受体(erα, erβ)以及雄激素受体(ar)的转录,可能通过这些受体途径参与生殖调控。研究结果表明环境相关浓度的左炔诺孕酮长期暴露可导致雄性斑马鱼的生殖内分泌干扰效应,并提示:仅雄鱼单独受孕激素暴露影响,也可能对鱼类种群的生殖产生深远影响。     

稀土元素的脑部蓄积性、毒性及其对人群健康的潜在危害

论述了稀土元素在动物脑部的蓄积性、对脑的毒性以及稀土区人群的脑部毒性效应,分析了稀土农业应用和稀土厂“三废”对生态环境和人群健康的潜在危害。     

大气苯酚对蚕桑污染毒性

本文以桑树和家蚕为试验生物，采用静态模拟熏气和自然污染调查相结合的方法，对大气苯酚污染蚕桑引起家蚕生长发育障碍进行了观察、分析，认为大气苯酚以间歇性较高浓度侵入蚕室时，会直接致蚕中毒；大气中苯酚以持续较低浓度作用时桑叶内会累积苯酚，间接对象蚕有毒害。家蚕受害后，轻则表现力食欲不振，群体发育不齐，体色灰暗、体重减轻、主要经济性状下降，重则死亡。，Ｆ＿（０．０１）＝２．８０说明因熏气处理次数引起盛食蚕体重变化的差异极显著，也即较高浓度的苯酚气会直接危害家蚕生长、发育。综上所述，在有关工厂附近大气内苯酚浓度虽然不是很高，也没有被正式列入监测范围，因此也没有监测指标。但此浓度下桑叶内会累积苯酚，家蚕食叶则会间接遭受其危害；如急性排放的苯酚气侵入蚕室，则会对家蚕直接致害，造成经济性状下降，甚至死亡。苯酚是有机毒物，食品上禁用早有规定，如何防止大气苯酚在生态系统中积累、迁移，保障陆地生物包括人类免受其毒害，是值得引起重视的环境问题。