Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE

This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis （DGGE） of 16S rRNA gene of bacterial strain, and found two dominant strains in the DGGE profile. Using special culture media, we isolated two strong cadmium-resistant bacterial strains. On the basis of morphological, physiological, and biochemical characteristics, BIOLOG, and 16S rDNA sequencing, the two strains were identified as Bacillus cereus and Enterobacter cloacae. Minimal inhibitory concentrations （MICs） of heavy metals for the bacteria were determined. E. cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B. cereus.     

Releasing characteristics of phosphorus and other substances during thermal treatment of excess sludge

The releasing characteristics of phosphorus,nitrogen compounds,organics,and some metal cations during thermal treatment of excess sludge were investigated.It was found that during heating not only phosphorus,but also nitrogen compounds,organics,and some metal cations could be released in abundance.The maximum orthophosphate (ortho-P) release of about 90 mg/L in concentration was observed at 50℃in 1 h.Except for volatile fatty acids (VFAs),comparatively little total nitrogen (TN),total organic carbon (TOC),and metal cations were released at the same time.Such results might favor further process of phosphorus recovery.VFAs were considerably released only at 50℃.Acetic,butyric,and propionic acid were the most abundant components in turn and their releasing profiles exhibited good linear relationship with time (R~2=0.9977,0.9624,and 0.8908,respectively).The concentrations of Mg~(2 ) and K~ increased with time and temperature during thermal treatment,but Ca~(2 ) decreased.The release of Mg~(2 ) and K~ agreed well with TP release (R~2=0.9892 and 0.9476,respectively).Temperature in the experimental range had very little impact on the linear relationships, especially of Mg~(2 ).Moreover,the parameter of mixed liquor suspended solids (MLSS) was found to be an important factor for thermal sludge treatment as the released ortho-P and total phosphorus (TP) at 50℃increased more than one-fold when MLSS was increased from 4000 to 8000 mg/L.     

On-line enrichment and determination of polycyclic aromatic hydrocarbons in atmospheric particulates using high performance liquid chromatography with fluorescence as detector

Seven polycyclic aromatic hydrocarbons (PAHs) in atmospheric particulates were determinated by high performance liquid chromatography (HPLC) with fluorescence detector using direction injection and an on-line enrichment trap column.The method simplified the sample pretreatment,saved time and increased the efficiency.With the on-line trap column,PAHs were separated availably even underground injecting 1.0 ml sample with relatively high column efficiency.The recoveries of the seven PAHs were from 85% to 120% for spiked atmospheric particulate sample.The limit of detection was 15.3-39.6 ng/L (S/N=3.3).There were good linear correlations between the peak areas and concentrations of the seven kinds of PAHs in the range of 1-50 ng/ml with the correlation coefficients over 0.9970.Furthermore,it also indicated that the method is available to determine PAHs in atmospheric particulates well.     

基于土壤侵蚀模型的滑波临界雨量估算探讨

针对滑坡临界雨量确定目前存在的问题,提出一种基于土壤侵蚀模型的滑坡临界雨量估算的新方法。该方法基本思路是：降雨引起土壤侵蚀,当土壤侵蚀达到一定强度时可诱发滑坡,因此利用土壤侵蚀模型可以推算滑坡临界雨量。以湖北省秭归县为例进行试验,从降雨-土壤侵蚀-滑坡的成灾机理入手,利用卫星资料、地理信息资料及降雨资料,计算降雨侵蚀力、土壤可蚀性、地形（坡长、坡度）、植被覆盖和土地利用类型等因子,基于USLE土壤侵蚀模型,计算滑坡发生时土壤侵蚀强度,通过分析多个滑坡个例,确定滑坡临界土壤侵蚀强度,再根据降雨侵蚀力与降雨量之间的关系,推算不同预警点滑坡临界雨量。相比以往仅仅分析灾情与降雨之间关系的传统方法,该方法有较为清晰的物理意义,实际业务中也易于实现,在滑坡预警预报中有较高实用价值     

Assessing the Eutrophication of Shengzhong Reservoir Based on Grey Clustering Method

Reservoir water environment is a grey system. The grey clustering method is applied to assessing the reservoir water enviromnent to establish a relatively complete model suitable for the reservoir eutrophication evaluation and appropriately evaluate the quality of reservoir water, providing evidence for reservoir management. According to China＇s lakes and reservoir eutrophication criteria and the characteristics of China＇s eutrophication, as well as certain evaluation indices, the degree of eutrophication is classified into six categories with the utilization of grey classified whitening weight function to represent the boundaries of classification, to determine the clustering weight and clustering coefficient of each index in grey classifications, and the classification of each clustering object. The comprehensive evaluation of reservoir eutrophication is established on such a foundation, with Sichuan Shengzhong Reservoir as the survey object and the analysis of the data attained by several typical monitoring points there in 2006. It is found that eutrophication of Tiebian Power Generation Station, Guoyuanchang and Dashiqiao Bridge is the heaviest, Tielusi and Qinggangya the second, and Lijiaba the least. The eutrophication of this reservoir is closely relevant to the irrational exploitation in its surrounding areas, especially to the aggravation of the non-point source pollution and the increase of net-culture fishing. Therefore, it is feasible to use grey clustering in environment quality evaluation, and the point lies in the correct division of grey whitening function     

基于层次分析法的企业安全文化评价指标体系研究

通过分析影响企业安全文化建设水平的各种因素,获得对安全文化建设水平进行现状评价的综合指标体系,应用层次分析法的基本原理构建企业安全文化评价层次模型,确定相关因子的影响力排序。结果显示:监督控制是影响我国企业安全文化建设的首要因素,其次是风险评估,而安全亲情氛围营造列为最后,基本符合我国现阶段企业安全文化建设现状。最后,确定了采用AHP与安全文化检查表相结合的方法评价企业的安全文化。通过实例分析表明,基于层次分析法的企业安全文化评价指标体系具有较强的实用性,可为企业制定安全文化建设战略目标提供有力依据;AHP与安全文化检查表相结合的评价方法,简便、准确度高、操作性强,值得推广应用。     

层次分析法及其在航空工业企业风险评价中的应用研究

结合历史事故统计及调查问卷分析,确定评价分析指标;应用层次分析法确定评估因素;建立较为完备的航空工业企业评价体系模型;对指标的权重进行综合的定量分析和计算,确定了航空工业企业评估指标的权重。应用实例表明:应用层次分析法确定评价指标权重,可以综合考虑影响事故各种影响因素,能较为客观、真实地反映被评价指标因素概况,易于实现计算机程序化,直观易懂,可操作性强,可在航空工业企业中推广应用。     

石化行业泄漏检测与修复评估指标体系的建立与应用

泄漏检测与修复(LDAR)是国际上通用的减少石化行业VOCs无组织排放的技术方法。在对天津市60套石化化工装置进行LDAR核查评估及专家咨询的基础上,将层次分析法(AHP)与模糊综合评价法(FCE)相结合,建立了一个4层次24个指标的评估指标体系,通过AHP确定指标权重,利用FCE进行模糊综合评分。以甲、乙两石化企业为例进行LDAR评估指标体系的验证,结果表明,该指标体系评估结果与实际LDAR核查评估结果一致,具有良好的实际应用价值。     

Effect of individual parameter changes on the outcome of the estimated short-term dietary exposure to pesticides

ABSTRACT

In 2015 a scientific workshop was held in Geneva, where updating the International Estimate of Short-Term Intake (IESTI) equations was suggested. This paper studies the effects of the proposed changes in residue inputs, large portions, variability factors and unit weights on the overall short-term dietary exposure estimate. Depending on the IESTI case equation, a median increase in estimated overall exposure by a factor of 1.0-6.8 was observed when the current IESTI equations are replaced by the proposed IESTI equations. The highest increase in the estimated exposure arises from the replacement of the median residue (STMR) by the maximum residue limit (MRL) for bulked and blended commodities (case 3 equations). The change in large portion parameter does not have a significant impact on the estimated exposure. The use of large portions derived from the general population covering all age groups and bodyweights should be avoided when large portions are not expressed on an individual bodyweight basis. Replacement of the highest residue (HR) by the MRL and removal of the unit weight each increase the estimated exposure for small-, medium- and large-sized commodities (case 1, case 2a or case 2b equations). However, within the EU framework lowering of the variability factor from 7 or 5 to 3 counterbalances the effect of changes in other parameters, resulting in an estimated overall exposure change for the EU situation of a factor of 0.87-1.7 and 0.6-1.4 for IESTI case 2a and case 2b equations, respectively.     

Making Progress? Reproducing Hegemony Through Discourses of “Sustainable Development” in the Australian News Media

While numerous studies have investigated discourses of “sustainable development” in a variety of institutional and academic contexts, media discourses on this issue have remained largely unexamined. But considering the intertwined nature of media and society, the construction of sustainable development in the news may be an important factor shaping future ecological and social outcomes. Proceeding from the thesis that the news is a site in which hegemony may be reproduced, this paper critically analyses discourses used to construct sustainable development in news texts published over the 10-year period 2004–2013 across the eight most widely circulated newspapers in Australia. The findings show that sustainable development news is confined to a narrow range of discourses that largely serve to perpetuate the status quo, which is broadly consistent with the media hegemony thesis. Finally, I discuss the implications of these findings, propose possible explanations, and explore avenues for future research.