Lake Van is the largest lake in Turkey. The lake limits lifespan due to its high pH and brackish water. For this reason, only a single species of fish (Van Fish) is living in the lake that has been adapted to these conditions. In the present study, we investigated the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA) level and DNA damage effect of bisphenol A (BPA) (10?7, 10?6 and 10?5?M) on primary gill cell culture of Van Fish for 24 and 48?h of incubation periods. TAS levels were not changed when compared to those of the control group, but TOS levels were decreased in both 24 and 48?h. The MDA level increased only at the highest concentration (10?5) at the end of 12 and 24?h (p?.05). DNA damage increased only at the 10?5?M concentration after 48?h. At the end of the experiment, BPA exposure caused lipid peroxidation and genotoxic effect. These results indicate that high levels of BPA exposure induced oxidative stress and DNA damage by time- and concentration-dependent fashion in the gill cell culture of Van Fish. Gill cell culture is a useful model for the rapid identification of the harmful effects of chemicals in the aquatic environment. 相似文献
In this research, epigenetic effects of bisphenol A (BPA) on human breast cancer MCF-7 cells were analyzed. Genome-wide DNA methylation and gene expression were analyzed in MCF-7 cells exposed to BPA (10?5 and 10?6 mol/L for 5 weeks). No significant changes in the global level of 5-methyl-2′-deoxycytidine and 5-hydroxymethyl-2′-deoxycytidine were observed. DNA methylation profiling analysis indicated that BPA exposure resulted in the hypermethylation of FOXK2, LKB1, LMX1A and CUGBP2 and the hypomethylation of PTPRN2, TRIM27, BCAS3 and ZNF423. Decreased expression of apoptosis genes (P38 and BCL2L1) and increased expression of chemokine (Cxcl2 and ccl20) were detected. Changes of these genes were speculated to affect the ERα-related cell growth as well as cell apoptosis. 相似文献
We designed photoelectrochemical cells to achieve efficient oxidation of rhodamine B (RhB) without the need for photocatalyst or supporting electrolyte. RhB, the metal anode/cathode, and O2 formed an energy-relay structure, enabling the efficient formation of O2– species under ultraviolet illumination. In a single-compartment cell (S cell) containing a titanium (Ti) anode, Ti cathode, and 10 mg·mL–1 RhB in water, the zero-order rate constant of the photoelectrochemical oxidation (kPEC) of RhB was 0.049 mg·L–1·min–1, while those of the photochemical and electrochemical oxidations of RhB were nearly zero. kPEC remained almost the same when 0.5 mol·L–1 Na2SO4 was included in the reactive solution, regardless of the increase in the photocurrent of the S cell. The kPEC of the illuminated anode compartment in the two-compartment cell, including a Ti anode, Ti cathode, and 10 mg·mL–1 RhB in water, was higher than that of the S cell. These results support a simple, eco-friendly, and energysaving method to realize the efficient degradation of RhB.
In light of rising cost of fossil fuels and fears of its depletion, coupled with the increase in energy demand and the rise in pollution levels, governments worldwide have had to look at alternative energy resources. Combining renewable energy generation like solar power with superior storage and conversion technology such as hydrogen storage, fuel cells and batteries offers a potential solution for a stand-alone power system. The aim of this paper was to assess the techno-economic feasibility of using a hybrid energy system with hydrogen fuel cell for application in an eco-house that will be built in Sultan Qaboos University, Muscat, Oman. Actual load data for a typical Omani house of a similar size as the eco-house was considered as the stand-alone load with an average energy consumption of 40 kW/day and 5 kW peak power demand. The National Renewable Energy Laboratory's Hybrid Optimisation Model for Electric Renewable software was used as a sizing and optimisation tool for the system. It was found that the total annual electrical energy production is 42,255 kW and the cost of energy for this hybrid system is 0.582 $/kW. During daylight time, when the solar radiation is high, the photovoltaics (PV) panels supplied most of the load requirements. Moreover, during the evening time the fuel cell mainly serves the house with the help of the batteries. The proposed system is capable of providing the required energy to the eco-house during the whole year using only the solar irradiance as the primary source. 相似文献