Microbiological characteristics in a zero-valent iron reactive barrier

Zero-valent iron (Fe⁰)-based permeable reactive barriertreatment has been generating great interest for passivegroundwater remediation, yet few studies have paid particularattention to the microbial activity and characteristics withinand in the vicinity of the Fe⁰-barrier matrix. The presentstudy was undertaken to evaluate the microbial population andcommunity composition in the reducing zone of influence byFe⁰ corrosion in the barrier at the Oak Ridge Y-12 Plantsite. Both phospholipid fatty acids and DNA analyses were usedto determine the total microbial population and microbialfunctional groups, including sulfate-reducing bacteria,denitrifying bacteria, and methanogens, in groundwater andsoil/iron core samples. A diverse microbial community wasidentified in the strongly reducing Fe⁰ environment despitea relatively high pH condition within the Fe⁰ barrier (up topH 10). In comparison with those found in the backgroundsoil/groundwater samples, the enhanced microbial populationranged from 1 to 3 orders of magnitude and appeared to increase from upgradient of the barrier to downgradient soil. Inaddition, microbial community composition appeared to change overtime, and the bacterial types of microorganismsincreased consistently as the barrier aged. DNA analysisindicated the presence of sulfate-reducing and denitrifyingbacteria in the barrier and its surrounding soil. However, theactivity of methanogens was found to be relatively low,presumably as a result of the competition by sulfate/metal-reducing bacteria and denitrifying bacteria because of the unlimited availability of sulfate and nitrate in the site groundwater. Results of this study provide evidenceof a diverse microbial population within and in the vicinity ofthe iron barrier, although the important roles of microbial activity, either beneficially or detrimentally, on the longevityand enduring efficiency of the Fe⁰ barriers are yet to be evaluated.     

evaluation of a measurement method for forest vegetation in a large-scale ecological survey

We evaluate a field method for determining species richness andcanopy cover of vascular plants for the Forest Health MonitoringProgram (FHM), an ecological survey of U.S. forests. Measurementsare taken within 12 1-m² quadrats on 1/15 ha plots in FHM.Species richness and cover are determined for four height classes(strata) within each quadrat and aggregated by stratum over the entireplot. We estimated (1) the agreement between experienced trainers andinexperienced technicians who collected the data on this survey(accuracy) and (2) the agreement among the technicians (precision) forresults on species richness and cover from 3 test plots at 3 timeintervals. The methods appear to be highly precise, although somediscrepancies with the values obtained by the trainers were found.Trainers found significantly more species in the ground stratum (0–0.6 m) and measured significantly more cover in the uppermost stratum(>4.9 m). The proportion of variation due to measurement error andtemporal variability was less than 13% for species richness (all strata)and cover (all but one stratum). This indicates that the method issuitable for monitoring changes in species richness and canopy coverfor a large-scale synoptic monitoring project such as FHM.     

论DNA分子标记在家畜遗传多样性保护中的应用

本文就ＤＮＡ分子遗传标记在家畜遗传多样性保护中应用的可行性及途径进行了探讨，提出了家畜遗传多样性标记辅助保护新策略。     

Degradation of Populus euphratica community in the lower reaches of the Tarim River, Xinjiang, China

IntroductionRiparian systems are aquatic-terrestrial ecotones withunique biotic , biophysical and landscape characteristics(Gregory, 1991 ; Naiman, 1997 ; Wiens , 2002) and areshaped by fluvial geomorphic processes . Riparian plantcommunities perform an i…     

Total nitrogen and community turnover determine phosphorus use efficiency of phytoplankton along nutrient gradients in plateau lakes

Numerous studies support that biodiversity predict most to ecosystem functioning, but whether other factors display a more significant direct impact on ecosystem functioning than biodiversity remains to be studied. We investigated 398 samples of the phytoplankton phosphorus resource use efficiency (RUE_P = chlorophyll-a concentration/dissolved phosphate) across two seasons in nine plateau lakes in Yunnan Province, China. We identified the main contributors to phytoplankton RUE_P and analyzed their potential influences on RUE_P at different lake trophic states. The results showed that total nitrogen (TN) contributed the most to RUE_P among the nine lakes, whereas community turnover (measured as community dissimilarity) explained the most to RUE_P variation across the two seasons. Moreover, TN also influenced RUE_P by affecting biodiversity. Species richness (SR), functional attribute diversity (FAD2), and dendrogram-based functional diversity (FDc) were positively correlated with RUE_P in both seasons, while evenness was negatively correlated with RUE_P at the end of the rainy season. We also found that the effects of biodiversity and turnover on RUE_P depended on the lake trophic states. SR and FAD2 were positively correlated with RUE_P in all three trophic states. Evenness showed a negative correlation with RUE_P at the eutrophic and oligotrophic levels, but a positive correlation at the mesotrophic level. Turnover had a negative influence on RUE_P at the eutrophic level, but a positive influence at the mesotrophic and oligotrophic levels. Overall, our results suggested that multiple factors and nutrient states need to be considered when the ecosystem functioning predictors and the biodiversity-ecosystem functioning relationships are investigated.     

农业资源利用模式间的转换及案例分析

目前我国农业仍然属于资源高耗低效型农业，而农业自然资源是非常有限的，为此必须实现农业资源的高效利用。本文从中观区域尺度上研究了农业资源利用模式及不同利用模式间的转换，通过案例分析认为，农业资源利用模式处于不断演替过程中，其演替具有明显的层次性；在演替过程中，物化资源投入水平不断增加，农业资源利用效率不断提高；与农业资源利用模式相适应的农业资源利用技术体系也具有多样性和层次性。     

中国多样的生态农业技术体系

中国生态农业的技术体系主要包括①农业环境综合整治技术;②农业资源的保护与增殖技术;③小流域综合利用技术;④立体种养技术;⑤庭院资源综合利用技术;⑥再生能源利用技术;⑦农业副产物再利用技术;⑧有害生物的综合防治技术。中国生态农业的技术体系继承了中国传统农业的精华,充分利用资源,合理保护资源,有效地连接农业的不同组分。文章介绍了中国近１０多年来成功应用这些技术的典型例子,并且指出这将有利于中国实践＂２１世纪议程＂和粮农组织的＂持续农业和农村发展宣言＂。     

铜离子冲击下活性污泥微生物多样性的分子生态学分析

采用基于 16SrDNA序列分析方法 ,在长度多态 (LPM)和 16SrDNA的GC含量二维水平分析了受重金属污染的活性污泥系统内细菌的优势种类和多样性 .设计的PCR引物可以将细菌分为三大类 :即 1)proteobacterialα&δ(变形杆菌α&δ) ,2 )pro teobacterialβ&γ(变形杆菌 β&γ) ,以及 3)flexibacter(屈桡杆菌 )和革兰氏阳性菌 .分析了未受重金属驯化和受重金属驯化的两类活性污泥系统在重金属作用下优势种和多样性的变化 ,结果显示未驯化的活性污泥系统多样性减少但优势种的变化微小 ,而驯化系统优势种有较大的变化 ,但多样性基本不变 .这一结果证实驯化有助于提高微生物对重金属的抗性