多源信息融合技术在内陆湖库水华预警中的研究进展与展望

面对水体富营养化愈来愈严重,水华爆发越来越频繁的严峻形式;为减少水华发生频率及由此造成的损失,开展水华预警已成当务之急。水华爆发是水体中营养盐的累积、气候条件与水力条件等众多因素非线性共同作用的产物;为此,有必要在众多水华影响因素的动态监测信息的融合基础上,寻求水华爆发与这些影响因素间的影射关系,这就需要信息融合技术。通过归纳总结多源信息融合技术在内陆湖库水华预警中应用的研究进展,结合目前我国水华预警的具体需求,提出建立基于多源信息融合技术的水华预警决策支持系统的研究前景与初步设想。信息融合技术为水华预警提供了一个良好的平台,它将与水华相关的不同信息源（水文、气象、水环境质量与环境遥感）所提供的局部不完整的观测信息加以集成与互补,消除多源信息之间存在的冗余和矛盾,形成对水华爆发环境相对完整的感知与描述;从而提高水华预警与应急响应决策的效率,提高预警信息的时间与空间分辨率,扩展信息的时空监测范围。     

Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass (Lolium multiflorum L.) grown in diesel contaminated soil

For phytoremediation of organic contaminants, plants have to host an efficiently degrading microflora. To assess the role of endophytes in alkane degradation, Italian ryegrass was grown in sterile soil with 0, 1 or 2% diesel and inoculated either with an alkane degrading bacterial strain originally derived from the rhizosphere of Italian ryegrass or with an endophyte. We studied plant colonization of these strains as well as the abundance and expression of alkane monooxygenase (alkB) genes in the rhizosphere, shoot and root interior. Results showed that the endophyte strain better colonized the plant, particularly the plant interior, and also showed higher expression of alkB genes suggesting a more efficient degradation of the pollutant. Furthermore, plants inoculated with the endophyte were better able to grow in the presence of diesel. The rhizosphere strain colonized primarily the rhizosphere and showed low alkB gene expression in the plant interior.     

基于模糊神经网络的机动车驾驶教练员职业适宜性研究

提出应用模糊神经网络系统,建构教练员职业适宜性的3个要素(即心理素质、驾驶技能和知识的表达阐述能力)的学习样本,分析"三要素"的8项特征参数指标——场依存性、速度估计能力、交通安全意识、简单反应、选择反应、跟踪能力、行车注意力、表达阐述能力等,使用K-均值法对实验样本进行初始分类,形成标准学习样本,使用该样本对所构建系统进行训练和调试。利用经调试训练后的系统,依据所测教练员的心理、心理参数对其职业适宜性进行评价。试验表明:建立的教练员职业适宜性仿真模型能取得很好的评价效果。     

碱熔融法合成NaA和NaX型粉煤灰沸石的品质表征

以粉煤灰为原料采用碱熔融法合成了2种单一沸石矿物种的NaA和NaX型沸石,并对产物的结构、性能和应用指标进行了详细表征.经x射线衍射和IR光谱分析,表明合成产物是无杂晶生成的NaA和NaX型沸石相;在扫描电镜观察下,产物分别具有NaA和NaX型沸石的立方体和八面体晶体骨架结构.DTA分析表明了合成产物中沸石水的存在,且产物热稳定性较好.通过对比,粉煤灰合成的NaA和NaX型沸石的比表面积达到了相应商品沸石的66.9%和83.6%;孔容为41.1%和70.2%;阳离子交换容量(CEC,cation exchange capacity)为82.93%和84.31%.通过比较化学组成表明,大规模应用合成产物不会对环境造成危害.     

面向灾害应用的遥感影像融合统一理论体系设计

由于缺乏统一的理论体系指导,目前遥感影像融合研究相对比较混乱。针对像素级融合统一理论框架的思想,指出了遥感影像融合研究的方向和思路,将像素级融合统一理论框架逐步向特征级和决策级推广,着重发展了一套遥感影像融合统一理论体系。在统一体系下,面向灾害监测应用,按照理论研究、方法研究和应用研究的3个层次进行该理论体系的设计。     

抑制斑点噪声的SAR与多光谱图像融合方法

通过将主成分变换与IHS变换相结合,避免了会导致SAR信息量损失的滤波过程,实现了一种可降低SAR斑点噪声影响的遥感影像融合算法。与主成分变换融合方法、IHS变换融合方法在主观视觉效果分析和客观统计参数两方面进行比较,新方法不仅很好地保留了多光谱影像的光谱信息,增强了结果影像的空间细节表现能力,而且有效地抑制了SAR斑点噪声,提高了结果影像的信息量与清晰度。     

甲基营养型酵母表达载体系统研究进展

酵母作为生产真核异源蛋白的宿主菌 ,具备了易于分子遗传操作 ,原核微生物的生长性质及真核蛋白的翻译后加工修饰等特点 .其中Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ (酿酒酵母 )是第一个作为异源蛋白基因表达系统并加以应用的酵母 .人们认为Ｓ .ｃｅｒｅｖｉｓｉａｅ并非表达外源基因的最理想的酵母宿主菌 .在一群优于Ｓ .ｃｅｒｅｖｉｓｉａｅ的表达菌中 ,甲基营养型酵母Ｐｉｃｈｉａｐａｓｔｏｒｉｓ研究得最多 ,最具应用潜力 .在 2 0世纪 70年代期间 ,Ｐ .ｐａｓｔｏｒｉｓ是单细胞蛋白 (Ｓｉｎｇｌｅ ｃｅｌｌｐｒｏｔｅｉｎ…     

黄河甘宁蒙段表层沉积物中总磷3种提取方法的比较

通过微波消解法、标准测试方法(SMT)和碱熔法分别测定了水系沉积物成分分析标准物质GBW07307a(GSD-7a)中的总磷(TP)含量,并分别采用3种方法对采集于黄河流域甘宁蒙段表层沉积物样品进行TP含量和样品加标回收率的测定。结果表明:测定标准物质的相对误差绝对值:微波消解法碱熔法SMT法;变异系数:微波消解法碱熔法SMT法。同时,微波消解法提取的表层沉积物TP含量的范围为634.3~909.2μg/g;SMT法TP含量的范围为627.1~889.5μg/g;碱熔法TP含量的范围为559.1~784.8μg/g。微波消解法的测定结果相对偏高,SMT法的测定结果相对偏低。样品加标回收率平均值:微波消解法SMT法碱熔法;变异系数:微波消解法碱熔法SMT法。综合测定标准物质中TP含量以及样品加标回收率实验结果的准确度和精密度,对于黄河甘宁蒙段表层沉积物,使用SMT法提取TP较优于微波消解法和碱熔法。     

Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat

In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 μg L⁻¹, the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 h of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time.     

Zebrafish embryos/larvae for rapid determination of effects on hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-interrenal (HPI) axis: mRNA expression

To identify and prioritize chemicals that may affect thyroid and adrenal/interregnal endocrine system and to reduce cost and animal use by conventional toxicity assay, an in vivo screening assay was developed using zebrafish embryos/larvae based on measurement of expression of genes that were suggested to play important roles in hypothalamic-pituitary-thyroid (HPT) and hypothalamic-pituitary-interrenal (HPI) axis. Model chemicals that could modulate HPT and HPI axis in adult fish were selected in assay validation, including anti-thyroid agent 6-Propyl-2-thiouracil (PTU) and cytochrome P450 11B (Cyp11b) enzyme inhibitor metyrapone (MET). Zebrafish embryos were exposed to different concentrations of model chemical from 4 h post-fertilization (hpf) to 5 d post-fertilization (dpf). Exposure to PTU increased mRNA expression of sodium iodide symporter (nis) and thyroglobulin (tg) involved in HPT axis, and MET treatment up-regulated all the mRNA expression tested involved in HPI axis by a compensatory mechanism. These results suggested that HPT and HPI axis were active upon chemical exposure at least at 5 dpf zebrafish. Furthermore, we studied the effects of PTU or MET on the cross-talk between HPT and HPI axis. The results demonstrated that PTU and MET could affect cross-talk responses in zebrafish embryos/larvae.