Validation of reliable and selective methods for direct determination of glyphosate and aminomethylphosphonic acid in milk and urine using LC-MS/MS

Simple high-throughput procedures were developed for the direct analysis of glyphosate [N-(phosphonomethyl)glycine] and aminomethylphosphonic acid (AMPA) in human and bovine milk and human urine matrices. Samples were extracted with an acidified aqueous solution on a high-speed shaker. Stable isotope labeled internal standards were added with the extraction solvent to ensure accurate tracking and quantitation. An additional cleanup procedure using partitioning with methylene chloride was required for milk matrices to minimize the presence of matrix components that can impact the longevity of the analytical column. Both analytes were analyzed directly, without derivatization, by liquid chromatography tandem mass spectrometry using two separate precursor-to-product transitions that ensure and confirm the accuracy of the measured results. Method performance was evaluated during validation through a series of assessments that included linearity, accuracy, precision, selectivity, ionization effects and carryover. Limits of quantitation (LOQ) were determined to be 0.1 and 10 µg/L (ppb) for urine and milk, respectively, for both glyphosate and AMPA. Mean recoveries for all matrices were within 89–107% at three separate fortification levels including the LOQ. Precision for replicates was ≤7.4% relative standard deviation (RSD) for milk and ≤11.4% RSD for urine across all fortification levels. All human and bovine milk samples used for selectivity and ionization effects assessments were free of any detectable levels of glyphosate and AMPA. Some of the human urine samples contained trace levels of glyphosate and AMPA, which were background subtracted for accuracy assessments. Ionization effects testing showed no significant biases from the matrix. A successful independent external validation was conducted using the more complicated milk matrices to demonstrate method transferability.     

Analysis of Integration Mode of Human Resources Development and Cultural Ecology in Hebei Province

People and culture coexist and human resources development and regional cultural ecology integrate.The present thesis for the first time puts forward the integration mode of human resources development and cultural ecology,argues that personnel innovation should be attracted by motive injection,open culture,resources integration,culture dilution,thinking blending and people-orientation and discusses the transmission mechanism for functions of integration mode of human resources development and cultural ecology from the aspects of cultural values,living styles and cultural industry.     

基于证据权重法的呼伦贝尔地区人为草原火险空间预测

应用证据权重法结合GIS空间分析,研究了呼伦贝尔地区人为草原火险。利用证据权重法定量化地分析了与人类活动密切相关的5个因子(乡村人口密度、载畜密度、居民点分布、城镇分布、公路网)与草原火点空间分布的关系,并建立了呼伦贝尔草原火险预测模型,据此在ARCVIEW的WofE扩展模块下生成一幅草原火险预测专题图,并将研究区划分为高、中、低三类风险区。研究表明:(1)呼伦贝尔草原火灾火点的空间分布与人类活动关系密切,尤其是乡村人口密度、公路网与居民点分布这三个因子,权重分别为0.5191,0.1945,0.2864;(2)证据权重法可以客观定量地表现出草原火险与人类活动的关系,因而可为草原火灾风险管理提供决策支持。     

Spatio-Temporal Distribution of Polycyclic Aromatic Hydrocarbons (PAHs) in Atmospheric Air of Tamil Nadu,India, and Human Health Risk Assessment

This study analyzed the seasonal distribution and the possible sources of polycyclic aromatic hydrocarbons (PAHs) in the atmospheric environment of Tamil Nadu, India. Passive air sampling was performed at 32 locations during the period from April 2009 to January 2010, and PAHs were quantified using a gas chromatograph-mass spectrometer. Analysis showed that the concentrations of PAHs were in the range of 5–47.5 ng/m³ with uniform distribution in urban areas in all seasons. Pre-monsoon season showed the highest cumulative concentration of PAHs in both agricultural and coastal areas. Among PAHs, phenanthrene, fluoranthene, and pyrene levels were found to be predominant in all the samples, contributing up to 36%, 35.5%, and 24.5% of total PAHs, respectively. The signature of the PAHs obtained through diagnostic ratio and principal component analysis revealed that diesel emissions was the probable source of PAHs in all locations. Based on Word Health Organization guidelines, the human lung cancer risk due to observed level of PAH concentration (i.e., PAHs exposure) is meager. However, the risk is predicted to be more in the coastal area during summer (18 individuals among 0.1 million people). To the knowledge of these authors, this report is the first on the seasonal analysis of PAHs using passive air sampling in India.     

Human exposure to heavy metals and possible public health risks via consumption of wild edible mushrooms from Slovak Paradise National Park,Slovakia

The contamination level of 92 samples (12 species) of wild edible mushrooms and underlying substrates with heavy metals (Cd, Cu, Hg, Pb and Zn) in the Slovak Paradise National Park that borders with a region of historical mining and processing of polymetallic ores, were determined. The collected samples were analyzed using of atomic absorption spectrophotometry. The metals were determined separately in hymenophore (H) and rest of fruit bodies (RFB). Bioaccumulation factor as well as ratio of metal content in H and RFB were calculated. Cadmium and lead contents in hymenophore exceeded statutory limits of the EU (Cd: 0.5 mg/kg dry weight (dw), Pb: 1.0 mg/kg dw) for edible mushrooms in 96% and 83% of the samples, respectively. The risk from the consumption of the collected mushroom species was calculated based on the provisionally tolerable weekly intake (PTWI) values, and the highest health risk arising with consumption of particularly Macrolepiota procera, Marasmius oreades and Russula vesca from the observed area was demonstrated. It was shown that average weekly consumption of tested mushrooms species results the threat of exceeding of PTWI limits in the case of cadmium values (by 164%, 86% and 4% of PTWI for M. oreades, R. vesca and R. puellaris, respectively) and of mercury (by 96% of PTWI for M. procera) but not lead.     

海运人为失误原因及对策分析

安全是海上交通永恒的话题。海上交通安全系统由“人-船-环境”组成,人是组成该系统的重要因素之一。在分析人为因素与人为失误的基础上,阐述了海运事故与人为失误的关系,对造成人为失误的各种影响因素进行识别与分析,并进一步从安全管理、安全教育和安全技术三个方面提出了海运中人为失误的预防对策,以期为船舶及航运公司评估船员安全操作水平和船员队伍安全质量提供理论依据,进而达到降低人为失误发生率、降低海运事故发生率、提高航海安全的目的。     

人为差错成因分析方法研究

控制甚至消除人为差错成因能从本质上降低人为差错的发生概率,从而提高系统安全性。因此,辨识和分析人为差错成因是一件非常必要而又十分重要的工作。针对该问题,在总结和分析现有的人为差错成因分析方法的基础上,提出了一种新的人为差错成因分析框架。在该框架中,将差错成因分为"操作者-系统-任务-环境-组织因素"5个方面进行分析,并提出了"从大类到小类"的层次化分析原则。为了尽量降低不同差错成因之间的关联,提出了以任务为中心,将差错成因分为任务相关和任务无关两大类因子的分类方式。最终共分析得出了34种不同的差错成因。该差错成因分类框架的提出,为人为差错概率量化以及差错规避措施的设计提供了依据。     

基于哈默“人的差错理论”的道路交通安全研究

以道路交通安全系统为背景,人的差错为研究方向,运用"哈默"人的差错分类理论,从机动车驾驶员和非机动车驾驶员、乘客及行人两种人群的差错角度,对2004—2006年3年间我国交通事故发生率和死亡人数进行较有针对性的分析归类和研究,进而分别提出基于主动性和被动性两方面的交通事故人因预防措施。通过"哈默"全新的分类方法,全面分析交通行为中人的差错类型,为道路交通事故预防体系的研究、建立以及进一步的发展,提供了有参考价值的基于人的行为机理的理论依据和合理化建议。     

人因失误与人不安全行为相关原理的分析与探讨

从人因失误的机理、分类和特点等方面,对人因失误与人不安全行为间的相关原理进行系统的对比分析与探讨,笔者认为,应从安全教育、技术培训、人机系统设计等方面预防人因失误,从建立和维持操作者对安全工作的兴趣、作业标准化、安全管理等方面来控制人的不安全行为。以煤矿斜井提升事故为例,说明人因失误与人不安全行为的判定原则和方法,从而为预防、控制人因事故提供理论依据。     

自动扶梯运行方式对地铁站台人员安全疏散的影响

地铁车站站台人员密集,客流量大,在火灾等应急条件下对人员安全疏散有较高的要求.目前自动扶梯在地铁车站中大量应用,其运行方式(上行、下行、停运、反转)对地铁车站内人员安全疏散有较大影响.利用人员疏散网络模拟软件EVACNT7对某地铁站台层人员向站厅层疏散进行数值模拟,研究了当自动扶梯正常上行时的输送能力,并与不同人流条件下的楼梯的输送能力进行了对比,得到动态折算系数.同时也研究了当自动扶梯停运作为疏散通道使用时的输送能力,并与人流条件优化时的疏散楼梯输送能力进行了对比得到静态折算系数.还研究了下行的自动扶梯在火灾等应急条件下反转上行以降低人员疏散时间,并给出了一种下行自动扶梯反转最迟完成时间的计算方法.研究工作可以为同类地铁站台人员安全疏散设计和应急预案制定提供参考.