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排序方式: 共有428条查询结果,搜索用时 243 毫秒
351.
活性污泥胞外聚合物中蛋白质的探针分析方法 总被引:2,自引:0,他引:2
应用微相吸附-光谱修正技术(Microsurface adsorption-spectral correction,简称MSASC),利用刚果红(Congo Red,简称CR)探针测定了活性污泥胞外聚合物(Extracellular polymeric substances,简称EPS)中蛋白质(Protein)的含量,与常用的Folin酚法比较,结果一致,蛋白加标回收率为94.5%~107.1%,相对标准偏差(RSD)<4.5%.对反应机理的分析和产物结合常数的计算结果表明,在pH 2.21时CR-Protein作用符合Langmuir单分子层吸附理论,产物的结合比79∶1,结合常数K=2.17×106,聚集形式为Protein·CR79,摩尔吸收系数ε664r=8.50×105L·mol-1·cm-1. 相似文献
352.
Phytotoxicity of cadmium on growing Arachis hypogaea L. seedlings was studied. Seeds were exposed to 25, 50, and 100 μmol/L CdCl2 concentrations, for a period of 10, 15, 20 and 25 d. The extent of damage to chlorophyll, protein, proline, nitrate and nitrite reductase, antioxidant enzyme activity in leaves and roots were evaluated after 10 d of cadmium stress. The higher concentration of cadmium (100 μmol/L) resulted (leaves and roots) total chlorophyll 91.01%, protein 79.51%, 83.61%, nitrate reductase 79.39%, 80.72% and nitrite reductase 77.07%, 75.88% activity decreased with increase in cadmium concentrations and exposure periods. Cadmium caused significant changes in the activity of antioxidative enzymes. Contrastingly Cd treated plant tissues showed an increase in proline 159.87%, 239.6%, gluthion reductase (GR) 337.72%, 306.14%, superoxide disumutase (SOD) 688.56%, 381.72%, ascorbate peroxidase (APX) 226.47%, 252.14%, peroxidase (POD) 72.19%, 60.29% and catalase (CAT) 228.96%, 214.74% as compared to control. Cadmium stress caused a significant increase in the rate of SOD activity in leaves and roots of plant species. Results show the crop A. hypogaea is highly sensitive even at very low cadmium concentrations. 相似文献
353.
Xiaoshan Zhou Wan Wei Xiaoran Duan Hui Zhang Xiaolei Feng Tuanwei Wang 《Journal of environmental science and health. Part. B》2020,55(6):525-529
AbstractTelomere length was found to be associated with omethoate exposure and polymorphisms in certain genes among occupational workers. However, whether the polymorphisms in telomere-binding protein genes influence telomere length remains unclear. To explore the correlation between telomere length and polymorphisms in telomere-binding protein genes, telomere length in peripheral blood leukocytes was determined by real-time quantitative polymerase chain reaction in 180 omethoate-exposed workers and 115 healthy controls. Polymorphisms in 10 pairs of alleles were detected using flight mass spectrometry or polymerase chain reaction–restriction fragment length polymorphism technique. The results showed that individuals with GG genotype in TRF1 rs3863242 had longer telomere lengths than those with AG?+?AA genotype in the control group (p?=?0.005). The multiple regression analysis suggested that both omethoate exposure (b?=?0.526, p?<?0.001) and TRF1 rs3863242 GG (b?=?0.220, p?=?0.002) were related to a longer telomere length. In conclusion, GG genotype in TRF1 rs3863242 is linked to prolongation of telomere length, and individuals with GG genotype are recommended to strengthen health protection in a Chinese occupational omethoate-exposed population. 相似文献
354.
大气污染对悬铃木叶绿素及抗氧化酶系的影响 总被引:6,自引:0,他引:6
大气污染会影响悬铃木叶片内叶绿素含量以及活性氧清除系统,致使植物体内叶绿素含量降低,抗氧化酶系统活性上升,并使植物体内丙二醛积累增加。交通污染对植物的影响程度与工业污染相当,应引起重视。绿化可减少空气污染,提高环境质量。 相似文献
355.
ABA对米槠和格氏栲种子发育的影响 总被引:7,自引:0,他引:7
郑郁善 《应用与环境生物学报》1999,5(5):444-449
采用酶联免疫分析法(ELISA) 提取和测定米槠和格氏栲发育过程中果实内ABA含量,并用不同浓度ABA喂养,测定果实不同发育期对外源ABA的敏感性.用放射性同位素法测定ABA对贮藏蛋白质的积累作用,结果表明:在米槠和格氏栲种子发育过程中内源ABA 含量逐渐上升,两者均在10 月5 日达到峰值,米槠为1648 ngg,格氏栲为1 468 ngg,随后ABA含量均逐渐下降.果实成熟采收时,c(ABA) 仅为高峰时的19.随着果实的发育对外加ABA的敏感性逐渐变小,c(ABA)=10-4 molL-1 可以完全抑制10 月25 日( 米槠) 和10 月15 日( 格氏栲) 前种子发芽,但对完全成熟的种子c(ABA)=10-2 molL-1 亦不能抑制发芽.ABA 对成熟前期胚的贮藏蛋白质合成无影响,但能促进成熟中后期胚的贮藏蛋白质的积累作用,ABA维持米槠和格氏栲贮藏蛋白质合成和积累作用表现在转录水平上. 相似文献
356.
Rai A Tripathi P Dwivedi S Dubey S Shri M Kumar S Tripathi PK Dave R Kumar A Singh R Adhikari B Bag M Tripathi RD Trivedi PK Chakrabarty D Tuli R 《Chemosphere》2011,82(7):986-995
World wide arsenic (As) contamination of rice has raised much concern as it is the staple crop for millions. Four most commonly cultivated rice cultivars, Triguna, IR-36, PNR-519 and IET-4786, of the West Bengal region were taken for a hydroponic study to examine the effect of arsenate (AsV) and arsenite (AsIII) on growth response, expression of genes and antioxidants vis-à-vis As accumulation. The rice genotypes responded differentially under AsV and AsIII stress in terms of gene expression and antioxidant defences. Some of the transporters were up-regulated in all rice cultivars at lower doses of As species, except IET-4786. Phytochelatin synthase, GST and γ-ECS showed considerable variation in their expression pattern in all genotypes, however in IET-4786 they were generally down-regulated in higher AsIII stress. Similarly, most of antioxidants such as superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) increased significantly in Triguna, IR-36 and PNR-519 and decreased in IET-4786. Our study suggests that Triguna, IR-36 and PNR-519 are tolerant rice cultivars accumulating higher arsenic; however IET-4786 is susceptible to As-stress and accumulates less arsenic than other cultivars. 相似文献
357.
358.
小麦waxy蛋白亚基1D-SDS-PAGE分离方法改良 总被引:9,自引:0,他引:9
对小麦waxy蛋白的提取技术和电泳后胶片的银染方法进行了改良:将无胚的半粒种子研磨成粉,用洗液浸泡2h代替蒸馏水浸泡过夜,并且去掉了用洗液反复洗涤和离心的程序;电泳后胶后的染色采用快速银染法代替常用的银染法,改良后的单向十二烷基酸钠聚丙烯酰胺胶电泳方法(1D-SDS-PAGE)具有省时、方便、经济和有效的特点,可适用于大批小麦材料的waxy蛋白亚基的分析,图1参5。 相似文献
359.
二氧化硫衍生物对小鼠心、肝、肺蛋白质的氧化损伤作用 总被引:5,自引:0,他引:5
研究了二氧化硫(SO2)衍生物--亚硫酸钠和亚硫酸氢钠混合物(分子比为3:1),对小鼠心、肝、肺中蛋白质的氧化损伤作用,探讨其分子作用机制.连续5 d对动物腹腔注射SO2衍生物,每天注射剂量为0、0.025、0.100、0.400 g·kg-1.用2,4-二硝基苯肼比色法测定不同器官组织中蛋白质羰基含量.结果表明,SO2衍生物染毒剂量为0.025、0.100 和0.400 g·kg-1时,小鼠心、肝、肺蛋白质羰基含量染毒组与对照组相比均呈现不同程度的增加,不同器官羰基含量增量大小的次序为:肝>肺>心;其染毒剂量与心、肝、肺蛋白质羰基含量之间存在明显的剂量效应关系(p<0.05),线性拟合确定系数分别为0.894、0.893和0.903.由此认为,SO2衍生物可引起小鼠心、肝、肺组织蛋白质的氧化损伤,以及不同组织器官的蛋白质氧化损伤程度不同. 相似文献
360.
A two-dimensional flow and transport model was developed for simulating transient water flow and nonreactive solute transport in heterogeneous, unsaturated porous media containing air and water. The model is composed of a unique combination of robust and accurate numerical algorithms for solving the Richards', Darcy flux, and advection-dispersion equations. The mixed form of Richards' equation is solved using a finite-element formulation and a modified Picard iteration scheme. Mass lumping is employed to improve solution convergence and stability behavior. The flow algorithm accounts for hysteresis in the pressure head-water content relationship. Darcy fluxes are approximated with a Galerkin and Petrov-Galerkin finite-element method developed for random heterogeneous porous media. The transport equation is solved using an Eulerian-Lagrangian method. A multi-step, fourth-order Runge-Kutta, reverse particle tracking technique and a quadratic-linear interpolation scheme are shown to be superior for determining the advective concentration. A Galerkin finite-element method is used for approximating the dispersive flux. The unsaturated flow and transport model was applied to a variety of rigorous problems and was found to produce accurate, mass-conserving solutions when compared to analytical solutions and published numerical results. 相似文献