全文获取类型
收费全文 | 10602篇 |
免费 | 769篇 |
国内免费 | 2287篇 |
专业分类
安全科学 | 431篇 |
废物处理 | 166篇 |
环保管理 | 1760篇 |
综合类 | 8022篇 |
基础理论 | 1065篇 |
环境理论 | 6篇 |
污染及防治 | 697篇 |
评价与监测 | 1062篇 |
社会与环境 | 391篇 |
灾害及防治 | 58篇 |
出版年
2024年 | 54篇 |
2023年 | 200篇 |
2022年 | 299篇 |
2021年 | 385篇 |
2020年 | 382篇 |
2019年 | 325篇 |
2018年 | 268篇 |
2017年 | 357篇 |
2016年 | 366篇 |
2015年 | 452篇 |
2014年 | 476篇 |
2013年 | 637篇 |
2012年 | 615篇 |
2011年 | 742篇 |
2010年 | 603篇 |
2009年 | 575篇 |
2008年 | 503篇 |
2007年 | 693篇 |
2006年 | 709篇 |
2005年 | 536篇 |
2004年 | 509篇 |
2003年 | 542篇 |
2002年 | 484篇 |
2001年 | 435篇 |
2000年 | 343篇 |
1999年 | 255篇 |
1998年 | 190篇 |
1997年 | 171篇 |
1996年 | 153篇 |
1995年 | 194篇 |
1994年 | 185篇 |
1993年 | 148篇 |
1992年 | 127篇 |
1991年 | 114篇 |
1990年 | 91篇 |
1989年 | 79篇 |
1988年 | 60篇 |
1987年 | 78篇 |
1986年 | 44篇 |
1985年 | 53篇 |
1984年 | 50篇 |
1983年 | 23篇 |
1982年 | 36篇 |
1981年 | 33篇 |
1980年 | 13篇 |
1979年 | 10篇 |
1978年 | 16篇 |
1973年 | 8篇 |
1972年 | 8篇 |
1971年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
221.
A multiple pregnancy of high rank may occur in a couple at risk for a Mendelian disorder. Prenatal diagnosis is hampered by the difficulty of (1) obtaining chorionic villi from each zygote arid (2) unequivocally relating each sample to the corresponding embryo. The calculation of the genetic risk according to the number of zygotes led us to propose a diagnostic strategy based on embryo reduction, a technique initially designed to improve the perinatal outcome of multiple pregnancies with normal embryos. We report a case in which this approach allowed rational use of first-trimester chorionic villus sampling in a quintuplet pregnancy at risk for non-ketotic hyperglycinaemia, resulting in the selective birth of unaffected twins. 相似文献
222.
We report the first prenatal diagnosis of an affected fetus with Chediak-Higashi syndrome (CHS). Diagnosis was accomplished via fetal blood sampling at 17 menstrual weeks and was confirmed after birth. Retrospective measurement of the largest acid phosphatase-positive lysosomes in cultured amniotic fluid cells and chorionic villus cells showed that in CHS these lysosomes are significantly larger than those in normal cells. This method may be used for prenatal diagnosis of CHS by amniocentesis and chorionic villus sampling (CVS). 相似文献
223.
Maguelone G. Forest Hervé Bétuel Philippe Couillin André Boué Michel David Daniel Floret René Francois Pierre Guibaud Henri Plauchu Raphaël Rappaport 《黑龙江环境通报》1981,1(3):197-207
Amniotic fluid (AF) levels of 17-hydroxyprogesterone (17OHP) and testosterone (T) were determined at 16–17 weeks in 17 pregnancies at risk for CAH and results compared to 75 normal controls. The fetus was predicted to be unaffected in 12 cases on the findings of normal AF levels of both 17OHP and T and the latter allowed a correct prediction of fetal sex in all instances. HLA typing confirmed normality in 12 cases revealing 5 carriers, 5 homozygous normal and 2 indeterminate. Steroid levels of the 2 groups were similar. Three fetuses were predicted to be CAH affected on unambiguously high levels of 17OHP and T (in female only). HLA typing was in agreement, and the diagnosis was confirmed in 2 abortuses and a female newborn by physical and hormonal studies. In the last 2 cases AF levels of OHP and T were normal but HLA (A/B/C) genotypes were identical to the CAH affected siblings. Normal physical and hormonal findings in the 2 aborted fetuses would exclude the possibility of an in utero virilizing form of CAH. The discrepancy could be explained on the basis that the fetuses had an allelic form of 21-hydroxylase deficiency or on the basis of recombination (not fully tested). It is concluded that a fully informative prenatal diagnosis of CAH should not rely entirely on HLA typing but on hormonal studies. 相似文献
224.
As a preliminary step to preimplantation diagnosis of sickle cell disease in unfertilized eggs or 8-cell embryos of heterozygous parents, we established quality control for detection of the mutant and normal alleles of the beta-haemoglobin gene using single buccal cells. Efficient polymerase chain reaction (PCR) amplification of a 680 base pair sequence of the beta-globin gene spanning the site of the sickle cell mutation was obtained for 79 per cent of single heterozygous cells. In 71 per cent of cases, both alleles were detected. With this current efficiency, we predict that a clinical preimplantation diagnosis at the 8-cell embryo stage could be carried out safely and reliably for a couple at risk of transmitting sickle cell disease to their children. 相似文献
225.
Diana W. Bianchi MD Gretchen K. Zickwolf Melissa C. Yih Alan F. Flint Ossie H. Geifman Marlena S. Erikson John M. Williams 《黑龙江环境通报》1993,13(4):293-300
Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction. 相似文献
226.
Lee P. Shulman MD Patricia L. Gordon Donald S. Emerson R. Sidney Wilroy Sherman Elias 《黑龙江环境通报》1993,13(5):403-409
We describe the prenatal diagnosis of isolated bilateral fetal microphthalmia in a woman at increased risk of having a fetus with microphthalmia. Ultrasound examinations at 161 and 19-5 weeks' gestation demonstrated bilateral fetal microphthalmia with no other associated structural defects. The patient elected to terminate her pregnancy at 19.5 weeks. Pathological evaluation of the products of conception obtained by dilation and evacuation confirmed the prenatal diagnosis of isolated bilateral fetal microphthalmia. 相似文献
227.
C. A. Dell'Agnola V. Tomaselli E. Teruzzi B. Tadini A. G. Coran Surgeon-in-Chief 《黑龙江环境通报》1993,13(7):629-632
The impact of prenatal sonographic diagnosis of oesophageal and gastrointestinal obstructions has been analysed over a 10-year period. Three groups of patients were evaluated. The first group consisted of 46 newborns with abnormal prenatal sonogratns, 41 of which were confirmed to have intestinal obstruction postnatally. The second group consisted of 17 neonates with normal prenatal sonograms who had intestinal obstruction postnatally. The third group included 56 newborns who did not undergo a prenatal sonogram but who had intestinal obstruction confirmed at surgery. Polyhydramnios without the appearance of a stomach on ultrasound was diagnostic of pure oesophageal atresia. Polyhydramnios with intestinal dilation was diagnostic of intestinal obstruction. Although surgery was performed earlier in the infants diagnosed prenatally with ultrasound, mortality was no less than in the group that did not undergo a prenatal sonogram, probably because of the high incidence of associated anomalies. 相似文献
228.
滇池流域农田氮、磷肥施用现状与评价 总被引:21,自引:0,他引:21
2001年6月至9月分别对流域内晋宁,呈贡,西山,官渡和嵩明调查了365家农户有关化肥施用情况,调查分析和计算表明,每年从种植业流失的化肥中纯氮(N)达2575t,纯磷(P)达218t,蔬菜和花卉地的单位面积氮流失量是水田和旱地的7-20倍。菜地年每公顷流失氮204kg,水田的氮流失量最低,平均每公顷每年流失10kg。磷的流失也以蔬菜,花卉地为最高,蔬菜和花卉地的单位面积磷流失量是水田和旱地的9-10倍。研究结果证明,大量的氮,磷化肥施用,通过地表径流和地下水流入滇池是造成其水体富营养化的重要原因之一^[6],随着农业和农村经济的发展,蔬菜,花卉等经济作物在滇池流域的种植面积将会逐年扩大,因氮,磷化肥的施用面造成的氮,磷流失量将会不断加大,为了实现减少氮,磷化肥对滇池的污染,应积极推广精确施肥,平衡施肥等科学施肥技术,提高化肥利用率,减少化肥流失。增施有机肥,降低化肥用量,同时提高农村居民的环境意识,加强环境管理,依法保护农村环境,控制农业面源污染。 相似文献
229.
多环芳烃的污染及其生物修复 总被引:12,自引:0,他引:12
多环芳烃(PAHs)是环境介质中普遍存在的难降解有机污染物,文中综述了目前国内外对PAHs的研究状况,重点阐述了它的来源分布、迁移转化、生物毒性、监测控制及其生物修复的研究进展等,从而为防治环境污染提供科学依据。 相似文献
230.