基于iTRAQ技术荧蒽降解菌的比较蛋白质组学分析

提取荧蒽不同诱导时间的红球菌BAP-1蛋白,应用iTRAQ技术结合LC-MS/MS对差异蛋白进行聚类以及生物信息学分析,以研究荧蒽高效降解菌的蛋白功能调控机理.结果表明：共鉴定到796个差异蛋白（差异倍数为2）,其中表达上调的有613个,表达下调的有183个.3个比对组（3d/1d、6d/1d和8d/1d）共同差异表达蛋白为111个（上调56个,下调55个）.通过COG、GO富集和pathway富集分析后发现绝大部分差异蛋白参与代谢和能量产生过程.在荧蒽诱导下上调关键蛋白有细胞色素C、三磷酸腺苷合成酶、二磷酸核苷等激酶,还有一些结合蛋白、脱氢酶、核糖体蛋白和趋化性蛋白等;下调显著的是5-甲基四氢蝶酰三谷氨酸-同型半胱氨酸甲基转移酶.这些蛋白共同组成蛋白互作网络调控荧蒽降解菌的一系列生命活动.     

Rates of environmental problem generation: Thoughts on a new research direction

Environmental scientists are continually discovering new and emerging environmental problems. There have been many studies of how a particular environmental problem has occurred, its costs to society, and its (potential) resolution. However, to date there have been few studies of the rate at which new environmental problems are generated by human technological innovations. In this note, I present a conceptual framework that will help quantify how many now-unknown problems we may expect to occur in a given sector. Two brief case studies illustrate the difficulty of finding appropriate dataset for fitting the full model, however. Policy implications of the conceptual framework are discussed, with different kinds of economic sectors requiring different approaches.     

基于Internet的交通道口事故监测管理系统的研究

传统的道路交通监测存在不足之处 ,相应的路口自动监测力量犹显薄弱。对诸如闯红灯 ,闯单禁行线 ,超速等交通违章行为缺乏现代化的监测、取证等必要手段。笔者提出基于Internet的道路交通违章监测系统 ,有效地利用先进的通信及计算机技术 ,为提高交通道口的管理水平 ,确认违章事故提供了手段 ,并对此系统进行深入地分析和设计 ,同时通过实例证明 ,该系统违章监测和事故确认具有的有效性和可操作性。     

城市公共场所人群拥挤踩踏事故分析

城市公共场所人群高度聚集、流动性大,紧急状态时常发生群死群伤的拥挤踩踏事故,造成大量人员伤亡和恶劣的社会影响。笔者首先提出拥挤踩踏事故分析的一般方法,并用群集指数来表征人群高度聚集这一参量;通过对近年来国内外人群拥挤踩踏事故的分析,认为该类事故以人群高度聚集为条件,由公众造成又危害公众,具有诱因众多、发生突然、难以控制等特点;强调事故预防和控制重点是建立事故应急机制,加强人群的管理和疏导等;同时从公共场所的性能优化设计、人群素质和群集密度、人群管理控制以及信息交流等方面分析其事故原因及提出相应对策,对预防此类事故发生,减少群集伤亡有重要意义。     

石油烃类降解菌在不同矿化度下的生长规律及去油效果研究

研究了不同盐浓度含油废水中石油烃类降解菌的生长情况以及对原油的去除效果。实验结果表明:在盐浓度低于15%的石油烃废水中,降解菌生长旺盛,且数量大;在盐浓度为15%～25%的石油烃废水中有较多菌量,但数量已明显减少;在盐浓度为30%以上的石油烃废水中几乎无细菌生长。培养时间为13h时,去油率可达到55%;随着培养时间的延长,原油去除率趋于平缓。高盐度石油烃废水中生存的微生物在处理含油废水方面具有很大的应用潜力。     

WATER SUPPLY OPTIONS IN A NEW MEXICO WATER PLANNING REGION1

ABSTRACT: The population in the Jemez y Sangre Water Planning Region of New Mexico has reached the point at which the demand for water exceeds available supplies, particularly when precipitation is below average, as has frequently occurred in recent years. The desire to develop a sustainable water supply that relies on renewable supplies in wet years and preserves the water in storage for times of drought motivated a diverse set of stakeholders in the region to participate in regional water planning. The planning effort culminated in development of the Jemez y Sangre Regional Water Plan, which was adopted by municipal and county governments in the region. The plan assesses and compares water supply and demand in the region and recommends alternatives for protecting and restoring the existing water supply and addressing the pending gap between supply and demand anticipated by the year 2060. To convey to decision makers the alternatives available to solve the future water shortage, option charts were developed to portray the amount of water that could be obtained or conserved through their implementation. The option charts show that the projected gap between supply and demand cannot be met through one alternative only, but will require a combination of alternatives.     

ADAPTIVE MANAGEMENT OF LARGE RIVERS WITH SPECIAL REFERENCE TO THE MISSOURI RIVER1

ABSTRACT: The technocratic approach for managing the Missouri River and other large rivers is not effective in resolving conflicts among competing uses of water and dealing with uncertainty about how river ecosystems respond to alternative management actions. Adaptive management offers an alternative way to address these and other issues. It has the potential to alleviate management gridlock and provide lasting solutions to management of the Missouri River and other large river ecosystems. In passive adaptive management, simulation models and expert judgment are combined to select a preferred management action. While passive adaptive management is relatively simple and inexpensive to use, it does not necessarily provide reliable information for making management decisions. Active adaptive management uses statistically designed experiments to test assumptions or hypotheses about ecosystem responses to management actions. It is best carried out by a collaborative working group. Active adaptive management has several advantages, but the inability to satisfy certain prerequisites for successful application makes it more difficult to implement in large river ecosystems. A second‐best approach is proposed here to select, implement, monitor, and evaluate a preferred management action and retain that action provided ecological conditions improve and socioeconomic indicators do not fall below established acceptability limits.     

APPLICATION OF THE HEC-4 MONTHLY STREAM FLOW SIMULATION MODEL1

ABSTRACT: The HEC-4 monthly stream flow simulation model, developed by the Hydrologic Engineering Center, Davis, California, is used to extend the available historical stream flow records in the Central Ohio area. The principal objective of this paper is to examine the effectiveness of the HEC-4 model in generating synthetic monthly flows. Important statistical parameters are evaluated in order to relate the statistical properties of the historical and generated flows. In doing so, it is observed that the mean, standard deviation, and skewness of the generated flows are consistently larger than the corresponding estimates based on historical flows. However, results show that these statistics, as well as the lag-1 serial correlation, are generally well maintained by the generated sequences. The degree to which any statistical dissimilarities would be critical, from an engineering design point of view, is demonstrated by utilizing their low flow characteristics. Estimates of reservoir safe-yields, based on a nonsequential mass-curve analysis of the historical and generated low flows, indicate a nominal difference in this particular study.     

DETERMINISTIC-SYSTEMIC RELATIONSHIPS IN OVERLAND FLOW ANALYSIS1

ABSTRACT: An analysis of storm runoff is presented, giving primary attention to deterministic-systemic relationships. So that system aspects can be emphasized, other aspects are kept simple; analysis is made for uniform rainfall on a strip of uniform width, limiting direct applicability to small areas. Two relationships between excess rain and time of concentration, one based on hydraulic parameters, the other on hydrologic characteristics, are combined in a solution for peak discharge. The hydraulic expression gives the interrelationship of time of concentration, excess rain, friction, length, and slope. The hydrologic relationship is achieved by converting ordinary intensity-duration curves to corresponding excess rain intensity-duration curves, thereby interrelating excess rain, time of concentration, and loss characteristics. The resulting solution for peak discharge allows for systemic feedback among both hydraulic and hydrologic parameters.     

Investigation of geothermal energy utilization for thermal regulation of aquaculture raceway

ABSTRACT

Aquaculture raceway temperature has a direct impact on the aquatic specie being reared. In regions that undergo significant seasonal temperature variations, the thermal management of the raceway temperature becomes a challenge, directly impacting the production yield. This study investigates a novel approach to regulate the raceway temperature in a sustainable way by utilizing geothermal energy. A numerical energy model was developed to simulate heat transfer in a geothermal system encompassing both the individual borehole heat exchangers and their thermal interactions. Simulations were conducted for different configurations of the geothermal system over a complete seasonal cycle. Results show that flow rate, number of boreholes and the borehole spacing influence the temperature of the fluid at the raceway inlet. An increase in the number of boreholes provided better thermal regulation but an increase in the flow rate through the boreholes provided less thermal regulation. A borehole spacing of 6 m was found to be appropriate to reduce thermal interference. It was also observed that an increase in the fraction of the fluid passed through the geothermal system enhances the overall thermal regulation, with higher thermal regulation at lower flow rates. Results show that when 100% of the fluid passed through a 64 boreholes geothermal system, the average regulated raceway inlet temperature was 23% higher in winter months and 16% lower in summer months at the flow rate of 21.5 L/s compared to than at 43 L/s.