Extraction of DNA from amniotic fluid cells for the early prenatal diagnosis of genetic disease

Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F₅₀₈ deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F₅₀₈ primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR.     

First-trimester prenatal diagnosis of severe alpha-thalassemia

By means of chorion biopsy together with restriction endonuclease analysis of fetal DNA, first trimester diagnoses were successfully made in 33 fetuses at risk for Bart's hydrops fetalis. Seven pregnancies with Hb H or hydrops fetalis were therapeutically terminated before 4 months of gestation. Of the 26 pregnancies intended to continue, 18 have come to term with normal deliveries; one with threatened abortion was terminated at the end of the first trimester and, seven are progresssing normally.     

钢绞线逐根张拉下锚墩基床沉陷所致锚索预应力损失问题

在预应力锚索锚墩下部地基岩土的基床系数较低情况下,采用钢绞线逐根张拉来施加预应力会导致较大的预应力损失以及各钢绞线较显著的预应力不均。基于锚墩基床岩土体的弹性压缩模型,理论探讨了钢绞线逐根对称循环张拉下的锚索预应力损失,及钢绞线的预应力均匀性问题。分析表明,基床系数越低,逐根张拉下的锚索预应力损失越大,且其所含各钢绞线预应力的均匀性越差;而同样荷载和基床系数下,锚索所含钢绞线根数越多, 逐根张拉后的预应力损失和不均匀性也越大。但采用分级循环张拉、正序张拉及调整、锚墩下部一定扩散角填石等工序及措施有助于减轻预应力损失和解决不均匀性问题。     

珠江口伶仃洋的海涂土壤资源

伶仃洋是珠江口东北部浅海湾,汇集珠江东四口门的来水,在潮间带及紧邻的潮下带(-5米以浅)形成大片海涂。土壤质地较粘重,有机质含量在1.5—2.2％之间,含盐量为0.2—1.5％,pH 为6.5—8.0,自然肥力较高,有很高的开发利用价值。因潮位带和生态条件不同,可分为 1.宜农围垦带,集中于西部浅滩,土壤含盐量较低,有机质和磷钾养分丰富,宜种水稻、甘蔗、香蕉等作物;2.水产养殖带,包括不宜围垦的潮间带和向海侧的潮下带,宜养鲻鱼、对虾、牡蛎等;3.城市、港口和工业填海造陆岸段,集中于东部浅滩。     

长春轻轨交通噪声环境影响评价

在分析轻轨交通特点基础上，对长春轻轨交通噪声环境影响进行了系统评价。评价结果表明：轻轨列车单独运行产生的噪声低于70dB，不超标；轻轨与铁路并行段，铁路交通噪声大于轻轨交通噪声，铁路噪声超标，应在并行段设置声屏障以保证交通噪声不超标；轻轨车内噪声比传统的有轨电车低14dB，有利于乘客身心健康，是较理想的城市交通工具。     

再燃脱硝的动力学模拟和组分影响分析

采用Gear算法模拟了再燃脱硝再燃区中化学反应过程，反应的热力学和动力学参数采用GRI3．0,模拟结果和实验结果作比较，说明该模拟可以用于预测再燃脱硝的基本过程。在模拟的基础上讨论了不同再燃燃料及其中的不同组分如HCN、NH3、H2和CO等对脱硝效果的影响。     

Wood materials used as a means to reduce greenhouse gases (GHGs): An examination of wooden utility poles

There has been growing concern over the build-up of greenhouse gase(GHGs) in the atmosphere, particularly carbon dioxide (CO₂), as acause of global warming. The IPCC Third Assessment Report (2001) suggests two ways in which the choice of materials could berelevant. First, some materials, particularly wood, have the advantage thatthey continue to hold carbon (C)in their cells even after being convertedto products. The implications of this feature are well researched. Second,an area that is not well researched relates to the different energyrequirements for producing similar products made with different materials. Using the findings of recent research, this paper compares the energyrequirements and C emissions of manufacturing a product using wood withthat of other materials. The case study of utility poles demonstrates thepositive C and global warming consequences of the lower energyrequirements of wood in the U.S., compared to other materials such assteel or concrete. It demonstrates that GHG emissions associated withutility poles are a small but significant percent of total US annual emissions. Wood utility poles are associated with GHG emission reductions of 163Terragrams (Tg) of CO₂ when compared with steel poles. This isabout 2.8 percent of US annual GHG emissions, which are estimated atabout 5.28 Petragrams (Pg) of CO₂ annually. Thus, the use ofwooden utility poles rather than steel results in a small but significantreduction in total US emissions.     

公路的环境美学与景观

公路建设作为人类实践创造活动之一，它为人们所提供的不仅仅是交通便捷与安全的享受，而且还应有视觉上的娱悦和满足。一条公路，可以吸引人们的视觉，也可以使人们熟视无睹。这取决于公路在周围风景中的实际布局及详细设计和维护保养的程度。和谐、连贯、稳定等公路景观评价和设计中常常使用的概念。公路对景观的影响是不可避免的，符合美学原则的减轻措施是公路景观恢复的有效手段。一条精心设计的公路应与周围环境和谐一致，应该充分利用地形和景色，包括一些当地最好的代表植物，使其融入自然。并且在可能的情况下，凭籍自己独到的设计和上乘的品质，使其成为一个旅游观赏景区。     

利用印染厂碱减量废水脱硫试验及其应用研究

介绍了化纤印染厂坯布前处理工序产生的碱减量废水作锅炉烟气SO2脱硫剂的试验研究结果，分析了碱减量废水脱硫反应过程及其机理，同时设计了一套经济适用的新型的文丘里旋流湿式脱硫除尘器。工业性试验运行结果表明，利用碱减量废水脱硫以废治废，经济效益和环境效益显著。     

用Microsoft Excel模糊综合法评价空气质量

模糊综合评价，可以对受多层次、多因素影响的事物进行综合评价。，同时介绍了用此法评价的空气质量、每一天各点位诸因子的数据Micmsoft Excel软件在模糊综合评价中的应用，并指出Microsoft Excel的利用实现了评价结果可视化。