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201.
Nancy S. Day Marija Tadin Angela M. Christiano Patricia Lanzano Sergio Piomelli Stephen Brown 《黑龙江环境通报》2002,22(8):686-691
Prenatal diagnosis of sickle cell diseases has been available for several years, and our laboratory has performed over 1000 prenatal diagnoses. However, currently available techniques are labor-intensive and time-consuming, and thus the diagnosis is delayed, making the mother's decision difficult. We describe a rapid, high-throughput technique based on the ligation assay coupled with automated capillary fluorescence detection. This new approach allows the diagnosis of both Hgb S and Hgb C to be available in a few hours. We have utilized this technique in 30 prenatal diagnoses and found it to be in complete agreement with the standard diagnoses. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
202.
Effect of organic carbon on nitrification effciency and community composition of nitrifying biofilms
HU Jie LI Daping LIU Qiang TAO Yong HE Xiaohong WANG Xiaomei LI Xudong GAO Ping.Center for Applied Environmental Microbiology Chengdu Institute of Biology Chinese Academy of Sciences Chengdu China..School of Life Science Sichuan University Chengdu China .Graduate University of the Chinese Academy of Sciences Beijing China 《环境科学学报(英文版)》2009,(3)
The effects of organic carbon/inorganic nitrogen(C/N) ratio on the nitrification processes and the community shifts of nitrifying biofilms were investigated by kinetic comparison and denaturing gradient gel electrophoresis(DGGE) analysis.The results showed that the nitrification rate decreased with an increasing organic concentration.However,the effect became weak when the carbon concentration reached a suffciently high level.Denitrification was detected after organic carbon was added.The 12 h ammonium remo... 相似文献
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205.
秦淮河流域中游地区两变量洪水风险分析 总被引:1,自引:0,他引:1
秦淮河流域地处长江下游,其中游地区主要位于南京市江宁区境内,受汛期强降雨、上游洪峰以及下游长江水位顶托影响,洪水灾害频繁。针对秦淮河流域中游地区暴雨洪水影响因素众多的特点,利用二维Gumbel模型,开展了基于暴雨与洪水水位两变量的洪水风险分析。结果显示,与单变量极值分布相比,两变量极值分布综合考虑了暴雨和洪水的不同频率特征,能够较为全面地分析水文极值事件的统计规律,从而使洪水风险分析更加符合实际情况。 相似文献
206.
Matthew S. MacLennan Cai Tie Kevin Kovalchik Kerry M. Peru Xinxiang Zhang John V. Headley David D.Y. Chen 《环境科学学报(英文版)》2016,28(11):203-212
Capillary electrophoresis coupled to mass spectrometry(CE–MS) was used for the analysis of naphthenic acid fraction compounds(NAFCs) of oil sands process-affected water(OSPW). A standard mixture of amine-derivatized naphthenic acids is injected directly onto the CE column and analyzed by CE–MS in less than 15 min. Time of flight MS analysis(TOFMS), optimized for high molecular weight ions, showed NAFCs between 250 and 800 m/z. With a quadrupole mass analyzer, only low-molecular weight NAFCs(between 100 and 450 m/z) are visible under our experimental conditions. Derivatization of NAFCs consisted of two-step amidation reactions mediated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC), or mediated by a mixture of EDC and N-hydroxysuccinimide, in dimethyl sulfoxide, dichloromethane or ethyl acetate. The optimum background electrolyte composition was determined to be 30%(V/V) methanol in water and 2%(V/V) formic acid. NAFCs extracted from OSPW in the Athabasca oil sands region were used to demonstrate the feasibility of CE–MS for the analysis of NAFCs in environmental samples, showing that the labeled naphthenic acids are in the mass range of 350 to 1500 m/z. 相似文献
207.
Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DCGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak. 相似文献
208.
针对风险多维属性问题及船舶航行多种风险表现,提出基于二维灰云模型的LNG动力船航行过程风险推理方法。针对LNG使用及船舶航行2种作业方式,采用系统方法识别安全风险影响因素体系,确定2种作业方式下各风险体系指标变化权重,描述LNG动力船航行过程中LNG使用和船舶航行发生事故概率及后果;通过引入云模型,综合计算2种作业方式下概率及后果组合风险,并采用灰云推理2种作业方式同时存在时LNG动力船航行过程风险。结果表明:LNG动力船航行过程中LNG使用和船舶航行风险耦合,风险程度受控;二维灰云模型可有效分析多维属性下风险评估问题,以及不同作业下风险耦合作用。研究结果可为船舶航行过程风险定量评估提供依据。 相似文献
209.
采用荧光光谱、红外光谱、平行因子(PARAFAC)分析及二维相关光谱(2D-COS)分析来解释Fe (III)与牛粪发酵DOM的络合异质性及机制.结果表明,PAFAFAC能够识别6个荧光组分,包括类蛋白(C2)、类富里酸(C1)、类腐殖酸(C4、C5)、类蛋白与类富里酸和类腐殖酸结合的荧光组分(C3、C6).2D-SYS-COS分析仅能识别类蛋白和类富里酸荧光物质,DOM中类蛋白荧光为主要的荧光组分.2D-COS分析表明,在334nm处的类富里酸荧光组分优先与Fe (III)离子发生络合作用,络合次序为334nm→306nm.DOM中能够优先与Fe (III)发生络合作用的为仲铵盐-NH2基团,各官能团组分与Fe (III)发生络合作用次序为2265→2771→1528→1310→1805→1479cm-1.双对数模型计算结果表明牛粪发酵沼液DOM能够与Fe (III)形成高化学稳定性的络合物,其络合常数在4.34~7.03之间,研究结果能够为沼液施用土壤金属离子的形态分布和迁移转化提供理论指导. 相似文献
210.
浸没式膜-生物反应器膜污染物中胞外聚合物的提取与分析 总被引:1,自引:0,他引:1
研究了胞外聚合物对膜生物反应器中膜污染物的贡献和影响,通过对膜表面的EDX分析,发现膜污染物以有机污染物为主;采用碱液离心提取法提取反应器污泥和膜污染物中的EPS,发现二者的EPS主要由相对分子量在12000以上的大分子物质组成,而且这些大分子物质基本上都是难降解的;以TOC表征EPS总量,并对其中多糖、蛋白质、DNA等组分进行测定,发现膜污染物中的EPS浓度是反应器污泥中的4倍左右,多糖类物质是首要的膜污染物,蛋白质对膜污染也起着重要的作用;反应器内DNA含量要高于膜污染物中,可证实DNA不是主要的膜污染物;通过电泳分析发现,二者大片段区间内的DNA的分子量几乎相同. 相似文献