降解苯酚优势菌的筛选分离和培养条件研究

从膨润土中筛选出可在含2 g/L苯酚的PDA培养基上生长的菌种,经过逐级驯化,得到1株可以在1 g/L苯酚的无机盐固体培养基上生长并降解苯酚的优势菌种HJ01,其对苯酚600 g/L降解率可达94%.该菌生长的适宜碳源和氮源分别为蔗糖和NH4Cl,温度为25 ℃,pH值范围为6-7.     

Observations on the influence of water and soil pH on the persistence of insecticides

Abstract

The pH‐disappearance rate profiles were determined at ca. 25°C for 24 insecticides at 4 or 5 pH values over the range 4.5 to 8.0 in sterile phosphate buffers prepared in water‐ethanol (99: 1 v/v). Half‐lives measured at pH 8 were generally smaller than at lower pH values. Changes in half lives between pH 8.0 and 4.5 were largest (>1000x) for the aryl carbamates, carbofuran and carbaryl, the oxime carbamate, oxamyl, and the organophosphorus insecticide, trichlorfon. In contrast, half lives of phorate, terbufos, heptachlor, fensulfothion and aldicarb were affected only slightly by pH changes. Under the experimental conditions described half lives at pH8 varied from 1–2 days for trichlorfon and oxamyl to >1 year for fensulfothion and cyper‐methrin. Insecticide persistence on alumina (acid, neutral and basic), mineral soils amended with aluminum sulfate or calcium hydroxide to different pH values and four natural soils of different pH was examined. No correlation was observed between the measured pH of these solids and the rate of disappearance of selected insecticides applied to them. These observations demonstrate the difficulty of extrapolating the pH dependent disappearance behaviour observed in homogeneous solution to partially solid heterogeneous systems such as soil.     

Phototransformation of herbicide metsulfuron methyl

Phototransformation of the herbicide metsulfuron methyl was investigated on glass surface under sunlight and ultraviolet (UV) light and compared with dark condition. The half-lives of metsulfuron methyl under UV light and sunlight were found to be 0.5 and 7.8 days respectively. Rate of phototransformation followed first order kinetics with significant correlation coefficient. The major photoproducts were identified as methyl-2-sulfonyl-amino-benzoate, 2-amino-6-methoxy-4-methyltriazine and saccharin (O-sulfobenzoimide). Various metabolites from this study were identified by high performance liquid chromatography (HPLC). Authentic samples required for HPLC comparison were prepared in laboratory and characterized on the basis of nuclear magnetic resonance (NMR) and infra red (IR) spectral data. These metabolites were also identified from metsulfuron methyl treated wheat field soil.     

Aflatoxin decomposition in various soils

Abstract

The persistence of aflatoxin in the soil environment could potentially result in a number of adverse environmental consequences. To determine the persistence of aflatoxin in soil, ¹⁴C‐labeled aflatoxin B₁, was added to silt loam, sandy loam, and silty clay loam soils and the subsequent release of ¹⁴CO₂ was determined. After 120 days of incubation, 8.1% of the original aflatoxin added to the silt loam soil was released as CO₂ ? Aflatoxin decomposition in the sandy loam soil proceeded more quickly than the other two soils for the first 20 days of incubation. After this time, the decomposition rate declined and by the end of the study, 4.9% of the aflatoxin was released as CO_2. Aflatoxin decomposition proceeded most slowly in the silty clay loam soil. Only 1.4% of aflatoxin added to the soil was released as CO₂ after 120 days incubation. To determine whether aflatoxin was bound to the silty clay loam soil, aflatoxin B₁ was added to this soil and incubated for 20 days. The soil was periodically extracted and the aflatoxin species present were determined using thin layer chromatographic (TLC) procedures. After one day of incubation, the degradation products, aflatoxins B₂ and G₂, were observed. It was also found that much of the aflatoxin extracted from the soil was not mobile with the TLC solvent system used. This indicated that a conjugate may have formed and thus may be responsible for the lack of aflatoxin decomposition.     

Rain‐washing of foliar deposits of Dimilin®WP‐25 formulated in four different carrier liquids

Abstract

Dimilin^® WP‐25, a wettable powder formulation of diflubenzuron (DFB) [1‐(4‐chlorophenyl)‐3‐(2,6‐difluorobenzoyl) urea], was formulated in four different carrier liquids, viz., water; a light petroleum paraffinic oil, ID 585; a heavy paraffinic oil, Sunspray^® 7N; and a 1:2 mixture of a light petroleum aromatic solvent (Cyclosol^® 63) and canola oil; to provide four end‐use mixtures, Dim‐W, Dim‐585, Dim‐7N and Dim‐Cy‐C respectively, each containing 28 g of DFB per litre. Balsam fir branch tips clipped from greenhouse‐grown seedlings, and sugar maple branch tips clipped from field‐grown young trees, were exposed to uniform‐sized droplets (ranging in diameters from 135 to 190 μm) of the four end‐use mixtures which were atomized using a monodispersed droplet generator. Droplets were collected on the fir and maple branch tips and the initial residue per g fresh weight of foliage was determined by high‐performance liquid chromatography (HPLC). The branch tips were exposed to cumulative rainfall of 3, 6 and 10 mm at an intensity of 5 mm/h and at time intervals of 1, 12, 36 and 72 h after DFB treatment, to test the influence of ‘ageing’ of foliar residues on rainfastness. Foliar samples were collected for residue determination just before the onset of rainfall, and at 0.5 h post‐rain. DFB was quantified by the HPLC method. In the case of fir foliage, the Dim‐W formulation was the most susceptible to rain‐washing and the rainfastness did not increase with the ageing period of foliar deposits. In contrast, the three oil‐based mixtures showed greater rainfastness depending upon the carrier liquid and the ageing period. Rainfastness decreased in the order of Dim‐Cy‐C > Dim‐7N > Dim‐585 > Dim‐W. In contrast, the data on maple foliage indicated that the ageing of deposits increased the rainfastness of all the 4 end‐use mixtures. Dim‐585 was the most susceptible to rain washing, and rainfastness decreased in the order of Dim‐W > Dim‐Cy‐C > Dim‐7N > Dim‐585.     

The effects of triclopyr on 2,4‐D mineralization in two soils

Abstract

The effects of the herbicide triclopyr (3,5,6‐trichloro‐2‐pyridinyloxyacetic acid) on the mineralization of 2,4‐D (2,4‐dichlorophenoyxacetic acid) in two soils which differed in their histories of prior exposure to the two herbicides were investigated. The relative effects of triclopyr on 2,4‐D mineralization and most probable numbers of 2,4‐D degraders were dependent upon the soil. Triclopyr was shown to increase 2,4‐D mineralization rates in a soil which had been exposed to both 2,4‐D and triclopyr, but decreased the mineralization rate of 2,4‐D and inhibited the increase of most probable numbers of 2,4‐D degraders in a soil that had not been directly exposed to either herbicide.     

石油工业废弃物处置及其生物治理趋势

鉴于我国石油工作者对石油工业废弃物的普遍关注，现在介绍生物治理的基本概念及ＳＰＥ石油工程师协会中有关含油污泥生物处置的途径及其突出优越性。生物液／固处理工艺（ＬＳＴ）是目前含油污泥处置现场最经济可行的处置方案。这种好氧的ＬＳＴ工艺过程，能够比较充分地代谢和降解石油污泥中的油和脂并大量除去多环芳烃类（ＰＡＨ）化合物及其它有机化合物，明显地降低了石油废弃物对人、畜的毒害程度。其工艺较简单，操作也简易，凡经此工艺处理后的废弃物不留残毒和后患，因此早已被世界上先进国家的炼油业视为处理含油污泥的有效选择了。石油工业废弃物的生物防治和生物降解工艺较大程度地领先于目前我国现场正在执行的各种处理含油废弃物的处理工艺。     

VARIABILITY IN THERMAL STRATIFICATION IN A RESERVOIR1

ABSTRACT: The thermal stratification characteristics of a flow-augmentation reservoir, Round Valley Reservoir, New Jersey, and attendant driving conditions were documented and analyzed for portions of three years. Substantial differences in the thermal stratification regime of the reservoir occurred in response to the documented changes in meteorological, operating, and light penetration conditions. The features of stratification that were affected included: the depth of the upper mixed layer, the average temperature of the epilimnion, the temperature gradient in the metalimnion, and the average temperature in the hypolimnion.     

Fiscal incentives for Australian bushland

The clearing of over 80% of the native vegetation from Australian agricultural areas has contributed significantly to the degradation classification applied to more than half this land. Soil erosion, siltation, and salinity damage continue to increase yearly. This situation not only threatens the productivity of the farm sector but has contributed to the estimated loss of 78 species of native flora, endangerment of an additional 2206 species, and the loss of 20 species of Australia's marsupials.Private returns diverge from social returns because the action (or inaction) of farmers has an impact upon others, both now and in the future. There is justification, therefore, for the public sector to intervene on behalf of society in an attempt to influence private decision making for the social good. This article argues for increased incentives from the public sector in Australia to encourage the voluntary cooperation of farmers to improve the balance between development and conservation. In contrast to the essentially temporary nature of man-made measures such as flood-mitigating capital works, increasing the area set aside to native bushland offers scope for the permanent stewardship of the resource—land.     

高频超声波降解4-氯酚的反应机理及试验研究

探讨了高频超声波（1.7MHz)降解4-氯酚的反应过程和反应机理，研究了高频超声波降解4-氯酚的效果及4-氯酚的初始浓度，高级氧化方法（AOPs)等因素对降解效果的影响。试验结果表明，高频超声波降解4-氯酚为一级反应，超声波空化效应在降解过程中起主导作用。超声波与其他氧化方法联用可大大提高降解效率。具有良好的工业应用前景。