微生物脱臭研究应用进展

随着恶臭气体污染成为环境污染的重大问题,对恶臭气体的控制和治理逐渐兴起。文章论述了用微生物法治理恶臭气体污染的基本原理,国内外研究现状以及主要工艺的类型和特点。讨论微生物法的主要影响因素,总结其存在的问题,并对发展趋势做了介绍。     

铁路运输含硫原油过程中H_2S挥发规律模拟试验研究

以塔河12区含硫原油为研究对象,通过模拟铁路运输原油工况,研究罐内原油中H_2S挥发规律.结果表明,铁路运输过程中原油里H_2S析出浓度与时间的关系近似为6次多项式,并建证了H_2S浓度随时间变化的曲线方程;对影响罐内原油中H_2S挥发的2个主要因素罐内原油温度和罐车振动强度进行了分析.结果表明,罐内原油温度是原油中H_2S挥发的主要影响因素,升高罐内原油温度可以明显增加H_2S的挥发浓度.加大罐车振动强度对原油中H_2S挥发有促进作用,但不明显.     

基于B/S+C/S的矿山安全标准化管理信息系统研究

首先介绍了安全标准化实施的关键步骤、技术特点及其在矿山的应用,通过现场调研、安全培训,危害辨识与风险评价、文件汇编等环节在山西某矿山建立了安全标准化管理信息系统,在此基础上开发了与之相配套的、基于B/S和C/S混合模式的、在局域网上运行的应用软件--矿山安全标准化管理信息系统.系统由统一信息发布平台、即时报警系统、安全记录管理系统、统计分析系统等14个子系统组成.系统的应用提升了矿山安全生产整体水平,实现了矿山安全标准化和信息化.     

辽河稠油热采现场硫化氢的防范与治理建议

硫化氢是一种剧毒和较强腐蚀性的气体,近年来在辽河油田一些地区相继出现,特别是稠油热采区块出现了较高浓度的硫化氢。硫化氢的存在已经威胁到油田的安全生产,并成为油气勘探开发过程中必须解决的问题。本文对辽河油气稠油热现场硫化氢的预防与治理提出了一些建议。     

“5·12”汶川地震灾区典型区域生态环境状况影响评价

“5·12”汶川特大地震发生后。带来的植被破坏、水土流失、次生地质灾害等,给灾区生态环境造成了极大的影响。本文重点以汶川县作为典型区域,运用“3S”技术,通过对汶川县震前、震后的生态环境状况及其变化情况进行定量评价。分析“5·12”大地震对龙门山区生态环境的影响,以期为区域灾后重建提供参考。     

Radionuclides in cigarettes may lead to carcinogenesis via p16 inactivation

It is widely accepted that tobacco smoke is responsible for the vast majority of lung cancers worldwide. There are many known and suspected carcinogens present in cigarette smoke, including α-emitting radioisotopes. Epidemiologic studies have shown that increased lung cancer risk is associated with exposure to ionizing radiation, and it is estimated that the majority of smoking-induced lung cancers may be at least partly attributable to the inhaled and deposited radiation dose from radioisotopes in the cigarette smoke itself. Recent research shows that silencing of the tumor suppressor gene p16^INK4a (p16) by promoter methylation plays a role in smoking-related lung cancer. Inactivation of p16 has also been associated with lung cancer incidence in radiation-exposed workers, suggesting that radionuclides in cigarette smoke may be acting with other compounds to cause smoking-induced lung cancer. We evaluated the mechanism of ionizing radiation as an accepted cause of lung cancer in terms of its dose from tobacco smoke and silencing of p16. Because both radiation and cigarette smoking are associated with inactivation of p16, and p16 inactivation has been shown to play a major role in carcinogenesis, ionizing radiation from cigarette smoke likely plays a role in lung cancer risk. How large a role it plays, relative to chemical carcinogens and other modes of action, remains to be elucidated.     

一株高效脱硫脱氨氮菌的分离、鉴定及系统发育分析

从运行稳定的能同步脱臭的曝气生物滤池中采集样品,富集分离获得一株能高效脱硫脱氨氮的菌株TS-1。对分离菌株进行形态观察、生理生化试验及16S rRNA基因序列分析,结果表明,该菌株TS-1为革兰氏阳性菌,杆状;菌落在营养琼脂培养基上呈圆形,表面光滑,乳白色半透明;V-P试验阴性,能水解淀粉和明胶,利用柠檬酸盐生长;对菌株进行16S rRNA的PCR扩增,扩增产物测序结果表明分离菌株TS-1与巨大芽孢杆菌（Bacillus megaterium）同源性达到99%;以16S rRNA同源性为基础构建了包括24株相关种属细菌在内的系统发育树,在系统发育树中,分离菌株TS1与Bacillus megaterium在同一分支。结合形态观察、生理生化试验及16S rRNA基因序列分析结果,将其初步鉴定为巨大芽孢杆菌（Bacillus megaterium）。在常温（30±2）℃、转速为150 r/min的条件下,处理pH 7、S^2-和NH⁺₄-N分别为80 mg/L和88 mg/L的水样40 h,硫化物和氨氮的脱除率分别为91.8%和96.6%。     

活性污泥体系中聚糖菌的富集与鉴定

活性污泥体系中,聚糖菌(GAOs)在厌氧环境下与聚磷菌(PAOs)形成对底物的竞争关系,对聚糖菌的研究对于优化生物除磷工艺有重要意义。以葡萄糖为惟一碳源,在磷限制条件下,利用特殊运行方式对活性污泥进行驯化培养出了稳定的聚糖菌颗粒污泥,厌氧阶段磷释放量与有机物吸收量浓度(mg/L)比从7.4%下降为0.25%。从培养好的活性污泥反应器中分离获得2株聚糖菌,经菌落PCR和16S rRNA序列分析确定了所得聚糖菌菌株G1和菌株G2分别是枯草芽孢杆菌(Bacillus subtilis)和解鸟氨酸克雷伯氏菌(Klebsiella ornithinolytica)。     

空气和废气监测中H2S标液的选择

有2种不同方法制备的H2S标准溶液进行测定,结果表明,所得标准曲线斜率一致,因此,在实际工作中可对其灵活运用。     

Comparison of ALAD activities of Citrobacter and Pseudomonas strains and their usage as biomarker for Pb contamination

Delta-aminolevulinate dehydratase (ALAD) activity has been used in prokaryotes and eukaryotes as a biomarker for environmental lead (Pb) exposure and toxicity. Microorganisms are sensitive indicators of toxicity at the fundamental level of ecological organization, but bacterial biomarker studies are focused on the Pseudomonas strains in Group I and E coli. The objectives of the present work were to determine if Burkholderia gladioli belonging to group II, due to its 16SrRNA similarity, can be used as biomarker in metal contamination and compare its possible usage with Pseudomonas aeruginosa and Citrobacter freundii (previously known as Esherichia freundii) and Bacterium freundii which are classified in Group I. In this study, ALAD activity in an environmental strains of Burkholderia gladioli, Pseudomonas aeruginosa, Citrobacter freundii were investigated to evaluate potential inhibition by Pb and other toxic metals. When the ALAD activity of Burkholderia gladioli was tested, Co and Pb decreased activity by 27 and 71%, respectively. In addition to these findings, Zn increased the activity up to 26%. These effects were found to be statistically meaningful (p < 0.05). It was determined that the increase of lead concentration inhibites the ALAD activity at each of the three strains. There was a statistically significant dose–response relationship between ALAD activity in cells of Burkholderia gladioli and Pb (Pearson correlation coefficent = −0.665; r ² = 0.665, and p < 0.001). The strongest ALAD inhibition which was measured was 90% at Burkholderia gladioli when protein extracts were incubated with 750 μM of Pb. The relationship between Pb and ALAD activity was statistically described by [ALAD Activity] = 0.476−0.000597 × [Pb]. According to the obtained results, we suggest that the ALAD of Burkholderia gladioli can be used as a biomarker for lead contamination in the environment.