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Triclosan is used as an antibacterial agent in household items and personal care products. Since this compound is found in maternal milk of humans and bodies of wild animals, there is growing concern among some consumer groups and scientific community that triclosan is adverse for humans and wild animals. In order to estimate adverse actions of triclosan, the effects of triclosan on intracellular Zn2+ concentration and cellular thiol content were studied in rat thymocytes by the use of flow cytometer with appropriate fluorescent probes. Triclosan at 1-3 μM (sublethal concentrations) increased the intensity of FluoZin-3 fluorescence (intracellular Zn2+ concentration) and decreased the intensity of 5-chloromethylfluorescein (5-CMF) fluorescence (cellular thiol content). Negative correlation (r = −0.985) between triclosan-induced changes in FluoZin-3 and 5-CMF fluorescences was found. Removal of external Zn2+ did not significantly affect the triclosan-induced augmentation of FluoZin-3 fluorescence, suggesting an intracellular Zn2+ release by triclosan. These actions of triclosan were similar to those of H2O2 and triclosan significantly potentiated the cytotoxicity of H2O2. Therefore, the results may suggest that triclosan at sublethal concentrations induces oxidative stress that decreases cellular thiol content, resulting in an increase in intracellular Zn2+ concentration by Zn2+ release from intracellular store(s). Since recent studies show many physiological roles of intracellular Zn2+ in cellular functions, the triclosan-induced disturbance of cellular Zn2+ homeostasis may induce adverse actions on the cells. 相似文献
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Zhizhuo Liu Zhemin Shen Shouyan Xiang Yang sun Jiahua Cui Jinping Jia 《Frontiers of Environmental Science & Engineering》2023,17(3):31
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Abdelaaty A. Shahat Gamal El-Barouty Rasmeia A. Hassan Fiza M. Hammouda Fawzia H. Abdel-Rahman Mahmoud A. Saleh 《Journal of environmental science and health. Part. B》2013,48(6):519-525
Seeds of Enterolobium contortisiliquum were subjected to steam distillation to obtain a light yellow essential oil in a yield of 3 ml/kg of seeds. The major components of the oil were identified using gas chromatography/mass spectrometry (GC-MS) and were furfural, limonene, linalool, estragole, carvone, and apiole with carvone representing more than 50% of the total composition. Antimicrobial activities of the essential oil were determined against four species of gram positive bacteria (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Micrococcus luteus) and two gram negative bacteria (Klebsiella pneumoniae, Serratia Marcescencs). The essential oil inhibited the growth of all tested bacteria but was most effective against the gram positive bacteria. Chemicals that are responsible for the antibacterial effect of the essential oil were determined using the bio-autography thin layer chromatography (TLC) technique. The active compounds responsible for the activity were found to be carvone and estragole. 相似文献
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Triclosan,a commonly used bactericide found in human milk and in the aquatic environment in Sweden 总被引:11,自引:0,他引:11
High levels of the commonly used, effective bactericide Triclosan was found in three out of five randomly selected human milk samples. It was also found in the bile of fish exposed to municipal wastewater and in wild living fish from the receiving waters of the three wastewater treatment plants. 相似文献
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一株拮抗烟草青枯病菌的真菌的筛选与鉴定 总被引:1,自引:0,他引:1
为寻找烟草青枯病的生防微生物,研究采用平板涂布法从重庆烟田健康土壤中分离根际微生物,纯化后得到了59株真菌。用滤纸片法从中筛选出1株对青枯雷尔氏菌(Ralstonia solanacearum)有稳定拮抗效果的真菌,其抑菌圈为10.0 mm。其抑制活性优于或接近于近期发现的其他生防微生物。测定该株活性真菌的全细胞脂肪酸组成,进行菌落和分生孢子形态学观察,以及rDNA ITS区测序,结果表明:该菌株全细胞脂肪酸属性未被收录于TSBA6脂肪酸数据库,而根据形态学观察结果,以及对所获rDNA ITS区序列的分析,鉴定该株真菌为烟曲霉(Aspergillus fumigatus)。 相似文献
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A facile fabrication and processing of cerium oxide-graphene oxide(CeO_2-GO) hybrid nanocomposites without the use of any surfactant or any organic solvents using chemical method and treatment with microwave irradiation technique are reported. In-situ hexagonal nano cerium oxide particles embedded on the layered surface of GO sheets were investigated for the photodegradation of dyes, removal of chromium Cr(VI) ions and against antibacterial studies. The results imply that hybrid nanocomposites shows enhanced 5-folds of photocatalytic activities in UV(ultraviolet) light irradiation and exhibited rapid efficiency in the elimination of chromium ion better than the pure GO and CeO_2, which are inhibited by competent photosensitive electron inoculation and controlling the electron–hole recombination. The synergetic effect of CeO_2-GO composites played a vital role in showing better results against model bacterium than GO and CeO_2 are due to higher physical interaction endorsed to the stress of membranes acute by piercing edges,large surface area, and higher adsorptive conditions of graphene oxide sheets tailored with ceria particles. The amount of charge transferred at the interface increases with the concentration of O atoms, demonstrating the interaction between CeO_2 and GO is much stronger than CeO_2 and GO are due to the decrease of the average equilibrium distance between the interfaces. The CeO_2-GO interface staggered band alignments existing between the CeO_2 surfaces and GO which shows an excellent synergism. The structure and morphology of composites were tested by X-ray diffraction(XRD), Fourier transform infrared(FTIR), Raman, X-ray photoelectron spectroscopy(XPS), and high-resolution transmission electron microscope(HR-TEM). 相似文献
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Biljana Todorović Emil Rekanović Miloš Stepanović Miroslav Kostić Mihajlo Ristić 《Journal of environmental science and health. Part. B》2016,51(12):832-839
Toxicity of twenty-two essential oils to three bacterial pathogens in different horticultural systems: Xanthomonas campestris pv. phaseoli (causing blight of bean), Clavibacter michiganensis subsp. michiganensis (bacterial wilt and canker of tomato), and Pseudomonas tolaasii (causal agent of bacterial brown blotch on cultivated mushrooms) was tested. Control of bacterial diseases is very difficult due to antibiotic resistance and ineffectiveness of chemical products, to that essential oils offer a promising alternative. Minimal inhibitory and bactericidal concentrations are determined by applying a single drop of oil onto the inner side of each plate cover in macrodilution assays. Among all tested substances, the strongest and broadest activity was shown by the oils of wintergreen (Gaultheria procumbens), oregano (Origanum vulgare), and lemongrass (Cymbopogon flexuosus. Carvacrol (64.0–75.8%) was the dominant component of oregano oils, while geranial (40.7%) and neral (26.7%) were the major constituents of lemongrass oil. Xanthomonas campestris pv. phaseoli was the most sensitive to plant essential oils, being susceptible to 19 oils, while 11 oils were bactericidal to the pathogen. Sixteen oils inhibited the growth of Clavibacter michiganensis subsp. michiganensis and seven oils showed bactericidal effects to the pathogen. The least sensitive species was Pseudomonas tolaasii as five oils inhibited bacterial growth and two oils were bactericidal. Wintergreen, oregano, and lemongrass oils should be formulated as potential biochemical bactericides against different horticultural pathogens. 相似文献
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Quanfu Yao Qinggele Borjihan Huihui Qu Yixuan Guo Ziying Zhao Long Qiao Ting Li Alideertu Dong Ying Liu 《环境科学学报(英文版)》2021,33(7):33-43
Disposal of the pollutants arising from farming cattle and other livestock threatens the environment and public safety in diverse ways. Herein, we report on the synthesis of engineered biochars using cow dung as raw material, and investigating these biochars as antibacterial agents for water decontamination. By coating the biochars with N-halamine polymer and loading them with active chlorine (i.e., Cl+), we were able to regulate them on demand by tuning the polymer coating and bleaching conditions. The obtained N-halamine-modified biochars were found to be extremely potent against Escherichia coli and Staphylococcus aureus. We also investigated the possibility of using these N-halamine-modified biochars for bacterial decontamination in real-world applications. Our findings indicated that a homemade filter column packed with N-halamine-modified biochars removed pathogenic bacteria from mining sewage, dairy sewage, domestic sewage, and artificial seawater. This proposed strategy could indicate a new way for utilizing livestock pollutants to create on-demand decontaminants. 相似文献
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The antibacterial triclocarban (TCC) concentrates in the cellular fraction of blood. Consequently, plasma levels are at least two-fold lower than the TCC amount present in blood. Utilizing whole blood sampling, a low but significant absorption of TCC from soap during showering is demonstrated for a small group of human subjects. 相似文献