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31.
32.
Mateos S Daza P Domínguez I Cárdenas JA Cortés F 《Environmental pollution (Barking, Essex : 1987)》2008,153(3):590-593
A field study was carried out in the south of the Iberian Peninsula in an industrial area in the neighbourhood of Huelva city, SW Spain, and in a natural area (Do?ana National Park) for comparison, to estimate the genetic risk induced by environmental pollution in wild mice. Genotoxic effects in a sentinel organism, the Algerian mice (Mus spretus) free living in the industrial area were compared with animals of the same species living in the natural protected area. The single cell gel electrophoresis, or Comet assay, was performed as a genotoxicity test in peripheral blood of mice. Our results clearly show that mice free living in the contaminated area bear a high burden of genetic damage as compared with control individuals. The results suggest that the assessing of genotoxicity levels by the Comet assay in wild mice can be used as a valuable test in pollution monitoring and environmental conservation. 相似文献
33.
A recently favored hypothesis is that duetting in birds has a mate-guarding function: a male responds vocally to his partner’s
song, thereby forming a duet that repels males who are attracted to her song. Previous studies have not provided unambiguous
tests of the mate-guarding hypothesis because: (1) the probability of a male answering his partner’s song has not been shown
to increase specifically when the female is fertile, and (2) the probability of a male answering his partner’s song has not
been assessed separately from simply a higher song initiation rate. We investigated extra-pair paternity, mate-guarding, and
duetting in the socially monogamous Australian magpie-lark (Grallina cyanoleuca). DNA fingerprinting revealed that 3% of young were the result of extra-pair paternity, and we found that males guarded fertile
females by staying close to them. However, males did not initiate songs at a higher rate when females were fertile and actually
reduced their probability of replying to female song during this period. We conclude that although male magpie-larks did guard
fertile females in an attempt to prevent extra-pair copulations, they did not use duetting for this purpose.
Received: 10 May 1999 / Received in revised form: 27 September 1999 / Accepted: 2 October 1999 相似文献
34.
Social and mating system of cooperatively breeding laughing kookaburras (Dacelo novaeguineae) 总被引:4,自引:0,他引:4
DNA fingerprinting was combined with field observations over four breeding seasons to investigate the social structure and
mating system of the laughing kookaburra (Dacelo novaeguineae). Groups comprised a socially dominant pair and up to six helpers of either sex. Helpers were always recruited from young
hatched in the group. Territorial inheritance, which is a feature of other cooperative breeders and an oft-cited benefit of
philopatry, did not occur. Helpers only attained dominant status in an established group by dispersing into a vacant dominant
position in that group. However, helpers could also form new groups by excising a new territory, often through a ”budding”
process. The mating system was overwhelmingly monogamous. There were no cases of extra-group parentage in a sample of 140
nestlings; within groups of three or more birds, dominance predicted parentage almost perfectly (99.2% of 129 nestlings),
irrespective of whether helpers in the group were related to one or both dominant birds. This is contrary to predictions from
models of reproductive skew, possibly because they currently fail to incorporate the willingness of females to share reproduction
among males.
Received: 15 May 1999 / Received in revised form: 2 November 1999 / Accepted: 6 November 1999 相似文献
35.
从顽拗植物荔枝中提取基因组DNA技术的研究 总被引:35,自引:0,他引:35
针对荔枝体内含有大量影响DNA提取质量的多酚等次生代谢物质的特点,在常规SDS和CTAB提取方法的基础上做了技术改进,即在核裂解之间先破碎细胞,将存在于细胞质中次生物质除去后再裂解细胞核,同时加入活性炭以吸咐杂质反复清洗几次后加入核型解液释放基因组DNA,纯化之后经外观和电泳检测,以及D260nm和D280nm比值的测定,限制性内切酶反应,PCR扩增的结果表明,用改进方法提取的DNA无论在纯度上还 相似文献
36.
针对S.xinjiangensis分类地位的争议,从新疆再次分离获得34株快生大豆根瘤菌,在16SrDNAPCR-RFLP分析和SDS全细胞蛋白电泳分群的基础上,进行了16SrDNA全序列相似性和DNA同源性分析.所测4个菌株和S.fredii模式菌株USDA205的16SrDNA全序列有很高的相似性.而DNA同源性分析说明新分离的菌株代表与原定的S.xinjiangensis为一个DNA同源群.其模式菌株CCBAU110与S.fredii的2株参比菌株USDA194、2048之间的DNA同源性分别为31.5%和28.7%.S.fredii的模式菌株USDA205与新分离的菌株代表及原定的S.xinjiangensis的模式菌株和2个参比菌株之间的DNA同源性为20.8%~39%,远低于70%.属于种水平上的差异.按照国际细菌分类委员会以DNA同源性≥70%且△Tm≤5℃作为定种的标准,S.xinjiangensis是Sinorhizobium属中不同于S.fredii的一个独立的种.表3参20 相似文献
37.
随着大量制冷装置的建成和使用,氨制冷企业安全问题日益突出,定期检验作为保证在役氨制冷压力容器安全使用的一个重要环节,目前仍存检验依据不清、检验项目不明、检验针对性不足等问题.文中对氨制冷压力容器定期检验现状进行了介绍,对氨制冷压力容器损伤机理进行了分析和讨论,从损伤模式判别的角度提出了相应的检验策略,给相关人员提供一些... 相似文献
38.
Eva Egelyng Sigsgaard Felipe Torquato Tobias Guldberg Frøslev Alec B. M. Moore Johan Mølgård Sørensen Pedro Range Radhouane Ben-Hamadou Steffen Sanvig Bach Peter Rask Møller Philip Francis Thomsen 《Conservation biology》2020,34(3):697-710
Conservation and management of marine biodiversity depends on biomonitoring of marine habitats, but current approaches are resource-intensive and require different approaches for different organisms. Environmental DNA (eDNA) extracted from water samples is an efficient and versatile approach to detecting aquatic animals. In the ocean, eDNA composition reflects local fauna at fine spatial scales, but little is known about the effectiveness of eDNA-based monitoring of marine communities at larger scales. We investigated the potential of eDNA to characterize and distinguish marine communities at large spatial scales by comparing vertebrate species composition among marine habitats in Qatar, the Arabian Gulf (also known as the Persian Gulf), based on eDNA metabarcoding of seawater samples. We conducted species accumulation analyses to estimate how much of the vertebrate diversity we detected. We obtained eDNA sequences from a diverse assemblage of marine vertebrates, spanning 191 taxa in 73 families. These included rare and endangered species and covered 36% of the bony fish genera previously recorded in the Gulf. Sites of similar habitat type were also similar in eDNA composition. The species accumulation analyses showed that the number of sample replicates was insufficient for some sampling sites but suggested that a few hundred eDNA samples could potentially capture >90% of the marine vertebrate diversity in the study area. Our results confirm that seawater samples contain habitat-characteristic molecular signatures and that eDNA monitoring can efficiently cover vertebrate diversity at scales relevant to national and regional conservation and management. 相似文献
39.
针对游乐设施行业危险源、损伤、失效和故障四个概念的认知差异,通过研究相关领域这四个概念定义内涵及外延,结合游乐设施安全保障要求高、人-机-环交互频繁、运行工况复杂的特点,重新定义游乐设施这四个概念,辨析相互之间的逻辑关系,并结合游乐设施事故案例进行解析.结果表明:新定义对游乐设施危险源、失效、损伤和故障之间的关系阐述更加清晰准确,为后续的风险评价奠定良好基础. 相似文献
40.
DNA宏条形码技术作为一种新型生物监测方法,在未来生态环境监测中有巨大的应用潜力。目前,浮游动物DNA宏条形码监测仍在发展阶段,需要首先对其(采样方法、引物选择和数据分析等)进行标准化和调整,然后才能用于常规流域生态监测。其中,如何选择合适的PCR扩增引物是DNA宏条形码生物监测标准化的关键问题之一。本研究比较了COI、18SV9和16S通用引物在浮游动物DNA宏条形码监测中的差异,为初步建立规范化的浮游动物DNA宏条形码监测方法提供技术支撑。结果表明,16S引物对浮游动物具有更好的特异性,其产生的操作分类单元(operational taxonomic unit, OTU)有88.1%属于浮游动物。虽然18SV9引物具有更高的物种覆盖度,不仅能扩增出浮游动物,还能扩增出大量藻类和真菌,但其物种识别敏感性较差,不适合浮游动物物种水平多样性监测。COI引物的浮游动物物种特异性、物种覆盖度和物种识别敏感性都适中,检出的浮游动物物种数量高于18SV9引物和16S引物,更加适合浮游动物DNA宏条形码监测。 相似文献