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761.
Aquatic organisms are exposed to fluctuating concentrations of herbicides which contaminate rivers following their use for agricultural or domestic purposes. The development of sensitive bioanalytical tests enabling us not only to detect the effects of those pollutants but to take into account this pattern of exposure should improve the ecological relevance of river toxicity assessment. In this respect, the use of chlorophyll fluorescence measurements is a convenient way to probe the effect of photosystem II (PSII) inhibitors on primary producers. This study was devoted to validate the combined use of two fluorescence parameters, the effective and the optimal quantum yields of PSII photochemistry (ΦPSII and Fv/Fm), as reliable biomarkers of initial isoproturon (IPU) or atrazine (ATZ) toxicity to natural periphyton in a pulse exposition scenario. ΦPSII and Fv/Fm were regularly estimated during a 7 h-exposure to each pollutant (0-100 μM) and also later after being transferred in herbicide-free water (up to 36 h). Our results showed that IPU was more toxic than ATZ, but with effects reversible within 12 h. Moreover, these two similarly acting herbicides (i.e. same target site) presented contrasted short term recovery patterns, regarding the previous exposure duration. 相似文献
762.
We tested the effects of the herbicide metsulfuron-methyl on growth of the submerged macrophyte Myriophyllum spicatum under laboratory conditions using different exposure scenarios. The exposures of each scenario were comparable in the concentration × time factor, viz., the same 21-d time-weighted average (TWA) concentrations but variable in peak exposure concentrations (ranging from 0.1 to 21 000 ng ai L−1) and exposure periods (1, 3, 7, 14 or 21 d). To study recovery potential of the exposed M. spicatum plants we continued the observation on shoot and root growth for another 21 d in herbicide-free medium so that the total observation period was 42 d. Non-destructive endpoints, length and number of new shoots and roots, were determined weekly from day 14 onwards. Destructive endpoints, dry-weight (DW) of main shoots, new shoots and new roots, were measured at the end of the experiment (t = 42 d).Metsulfuron-methyl exposure in particular inhibited new tissue formation but was not lethal to main shoots. On days 21 and 42 after start exposure, EC10/EC50 values for new tissues expressed in terms of peak concentration (=measured concentration during exposure periods of different length) showed large differences between exposure scenarios in contrast to EC10/EC50 values for days 21 and 42 expressed in terms of 21-d and 42-d TWA concentrations, respectively. At the end of the experiment (day 42), 42-d TWA ECx values were remarkably similar between exposure scenarios, while a similar trend could already be observed on day 21 for 21-d TWA ECx values. For the macrophyte M. spicatum and exposure to the herbicide metsulfuron-methyl the TWA approach seems to be appropriate to use in the risk assessment. However, the data from the toxicity experiment suggest that on day 21 also the absolute height of the pulse exposure played a (minor) role in the exposure - response relationships observed. 相似文献
763.
The Viciafaba root tip micronucleus test is one of the most employed plant genotoxicity assays, and has been used on various types of contaminated materials. This test has been standardized by AFNOR, the French member organization of ISO. However, this test is usually performed with a water extraction step but soil genotoxicity assessment would be more relevant when performed directly in the soil itself. In order to harmonize these protocols, an ISO standard for the V.faba micronucleus test in both liquid phase (exposure of plants to different liquid matrix, including soil water extracts) and solid phase (direct exposure of plants to the soil) would be very useful. In this context, we compared two exposure durations in the solid phase (48 h and 5 d) for the V.faba micronucleus test with two different well-known genotoxicants, maleic hydrazide and copper sulfate. We concluded that these two durations induced equivalent sensitivity: the micronucleus frequency was significantly increased with 5 μmol maleic hydrazide per kg dry soil and with 2 mmol copper sulfate per kg dry soil with both exposure durations. However, exposing roots to soil during 48 h is more practical. Moreover, organically and conventionally cultured seeds were employed to determine whether the seed provenance influenced the test sensitivity. Organic seeds were less sensitive to copper, possibly because copper-based treatments are permitted, and often applied, in organic farms. Therefore, in the absence of completely non-treated seeds, organically-cultured seeds did not appear to offer any advantages over conventional seeds. 相似文献
764.
Navarro A Faria M Barata C Piña B 《Environmental pollution (Barking, Essex : 1987)》2011,159(1):100-107
Development of stress markers for the invader freshwater zebra mussel (Dreissena polymorpha) is of great interest for both conservation and biomonitoring purposes. Gene expression profiles of several putative or already established gene expression stress markers (Metallothionein, Superoxide dismutase, Catalase, Glutathione S transferase, Glutathione peroxidase, Cytochrome c oxidase, the multixenobiotic resistance P-gp1, and heat shock proteins HSP70 and HSP90) were analyzed by quantitative Real-Time PCR in adults and pediveliger larvae after exposure to metals (Hg, Cu, Cd). A defined pattern of coordinated responses to metal exposure and, presumably, to oxidative stress was observed in gills and digestive gland from adults. A similar, albeit partial response was observed in larvae, indicating an early development of stress-related gene responses in zebra mussel. The tools developed in this study may be useful both for future control strategies and for the use of zebra mussel as sentinel species in water courses with stable populations. 相似文献
765.
Nickel sulfate induces numerous defects in Caenorhabditis elegans that can also be transferred to progeny 总被引:4,自引:0,他引:4
Whether the multiple biological toxicities from nickel exposure could be transferred to progeny has not been clarified. In this report, we explored the Caenorhabditis elegans to analyze the multiple toxicities of nickel and their possibly transferable properties. The nickel toxicity caused multiple biological defects in a concentration-dependent manner. Moreover, most of these toxicities could be transferred and could be only partially rescued in progeny. Some specific phenotypes in progeny were also found to exhibit no obvious rescue phenotypes or to show even more severe defects than their parents. The defects caused by nickel exposure could be classified into four groups according to their transferring properties. That is, the defects caused by nickel exposure could be largely, or partially, or unable to be rescued, or became even more severe in progeny animals. Therefore, most of the nickel exposure-caused defects can be transferred from parents to their progeny to different degrees in C. elegans. 相似文献
766.
Interactive effects of O3 exposure on California black oak (Quercus kelloggii Newb.) seedlings with and without N amendment 总被引:1,自引:0,他引:1
We examined the short-term separate and combined effects of simulated nitrogen (N) deposition (fertilization) and ozone (O(3)) exposure on California black oak seedlings (Quercus kelloggii Newb.), an ecologically important tree of the San Bernardino Mountains downwind of Los Angeles. Realistic concentrations of O(3) were found to cause statistically and biologically significant negative effects on plant health, including lowered photosynthetic ability, lowered water use efficiency, and increased leaf chlorosis and necrosis. When subjected to abrupt changes in light levels, O(3)-exposed plants showed both a slower and smaller response than O(3)-free plants. Fertilized plants exhibited a significantly greater pre- to post-treatment decline in A at saturated [CO(2)] and a significantly lower level of post-treatment chlorosis than unfertilized plants. Fertilization tended to reduce plant sensitivity to O(3). 相似文献
767.
Risk perception, risk management and vulnerability to landslides in the hill slopes in the city of La Paz, Bolivia. A preliminary statement 总被引:3,自引:0,他引:3
Nathan F 《Disasters》2008,32(3):337-357
The article begins by describing the difficult living conditions of many people in the hill slopes (laderas) of La Paz, Bolivia, demonstrating that they are exposed to a combination of natural and social hazards. 1 It shows that residents, community leaders and city planners tend to underestimate or deny risk, with important consequences for risk management, such as a failure to raise risk awareness. The article then proposes some hypotheses to explain risk perceptions in La Paz, discarding the usual single-approach interpretations and suggesting instead more nuanced theoretical explanations to account for why people build their homes in such hazardous environments. 相似文献
768.
水体低氧的早期暴露对青鳉(Oryzias latipes)后期的生长、性别比和繁殖能力的影响 总被引:1,自引:0,他引:1
水体低氧已是全球性生态问题,常以季节性、偶发性和昼夜间等不同形式存在于不同的水体中。长期低氧可影响鱼类正常的生长和繁殖,但鱼类早期生活阶段暴露于不同形式的低氧后,后期的生长和繁殖是否会受到不利影响,目前研究甚少。本研究在实验室模拟了连续低氧(2.8 mg·L~(-1)DO)(H1)、昼夜低氧(H2)和发生在胚胎器官形成时期的偶发性低氧(H3)等3种情景对青鳉胚胎的发育影响,评估了这一早期暴露对青鳉后期的生长、存活和繁殖的影响。我们发现,3种低氧方式都可以显著延长青鳉胚胎的孵化周期,引起胚胎卵黄囊吸收和鱼鳔发育异常;暴露结束120 d后,H1组青鳉成鱼的畸形率显著升高、存活率和生长速度都显著下降;H1、H2和H3组中成鱼的雌雄比都发生了改变,鱼群中以雄鱼为主,且产卵量和受精率都显著下降。结果表明,鱼类早期胚胎发育阶段所受到的低氧暴露可对后期生长和繁殖产生不利影响,对子代补充和种群稳定产生重要影响;鱼类关键发育期所经历的低氧事件,以及昼夜低氧事件所产生的生态后果不容忽视。 相似文献
769.
770.
家庭灰尘是人群居住场所的重要环境介质,人群可通过吸入、摄食及皮肤接触等途径摄入灰尘中的污染物,对健康造成一定的损伤。为探索我国西南某矿区周边村庄室内重金属的污染对人群健康的潜在风险,采用2014年3月对我国西南某矿区周边村庄室内灰尘中重金属污染水平调查的数据,结合美国EPA健康风险评价模型对人群健康风险进行分析。结果表明:人群非致癌风险和致癌风险暴露剂量均表现为儿童>成人男性>成人女性,呈现手-口摄食途径>皮肤接触途径>吸入途径,其中儿童、成人男性和成人女性手-口摄食暴露途径占总暴露剂量的98.16%、55.61%和51.5%;儿童以Pb、Cr的暴露为主,成人以Cr的暴露为主;家庭灰尘中单种重金属元素和多途径叠加的非致癌风险在8.81E-03~1.24E-02之间,均小于1,其中儿童以Pb的非致癌风险为主,经手-口摄食暴露占总风险的40%;致癌风险在4.19E-05~3.35E-04之间;A、B、C村儿童的致癌风险均高于US EPA所推荐的可接受水平10-4,其暴露剂量和健康风险均为成人男性和成人女性的5~6倍,且以Cr通过手-口摄食暴露产生的风险最大,占总风险的93%左右。 相似文献