Differences in response of two model estuarine crustaceans after lethal and sublethal exposures to chlorpyrifos

This study assessed the in vitro and in vivo effects of an acetylcholinesterase enzyme inhibitor (chlorpyrifos) in two estuarine crustaceans: grass shrimp (Palaemonetes pugio) and mysid (Americamysis bahia). The differences in response were quantified after lethal and sublethal exposures to chlorpyrifos and in vitro assays with chlorpyrifos-oxon. Results from the in vitro experiments indicated that the target enzyme, acetylcholinesterase (AChE), in the two species was similar in sensitivity to chlorpyrifos inhibition with IC50s of 0.98 nM and 0.89 nM for grass shrimp and mysids, respectively. In vivo experiments showed that mysids were significantly more sensitive to chlorpyrifos-induced AChE inhibition after 24 h of exposure. The in vivo EC50s for AChE inhibition were 1.23 μg L^?1 for grass shrimp and 0.027 μg L^?1 for mysids.

Median lethal concentrations (24h LC50 values) were 1.06 μg L^?1 for grass shrimp and 0.068 μg L^?1 for mysids. The results suggest that differences in the response of these two crustaceans are likely related to differences in uptake and metabolism rather than target site sensitivity.     

Impact of Soil Management and Two Botanical Insecticides on Urease and Invertase Activity

Yard waste compost provides an organic amendment useful for improving soil structure and nutrient status. The activities of the enzymes hydrolyzing urea (urease) and sucrose (invertase) in the rhizosphere of potato plants were determined under field conditions. Soil urease and invertase activities were monitored in compost amended soil, grass buffer strips, and in adjacent bare soils during 35 d following soil treatment. Soil urease activity was increased by application of yard waste compost compared to untreated soil which provide evidence of increased soil microbial population following application of compost. Some transitional effects on urease activities were observed following Pyrethrins and Neemix‐4E application, these effects were neither drastic nor prolonged enough to be considered deleterious to the soil microorganisms and their activities important to soil fertility. No relationship was found between invertase activity and the three management practices or soil organic matter content. It is suggested that application of botanical insecticides like pyrethrins and Neemix‐4E may be useful in delaying urea fertilizer mineralization to maintain N in a form less leachable, so that the duration of N availability to plants is prolonged. The present study may also provide information on urease activity as a sensitive bioindicator of soil quality that reflects the effects of land management on soil quality and may assist land managers in monitoring long‐term productivity and sustainability of agricultural lands.     

Reduced nitrogen has a greater effect than oxidised nitrogen on dry heathland vegetation

We investigated the effects of different ratios of reduced (NH4+) versus oxidised (NO3(-)) nitrogen in deposition on heathland and species-rich grassland vegetation at high nitrogen deposition levels in large mesocosms filled with nutrient-poor soils to which different NH4+/NO3(-) ratios were applied. The response of the forbs, Antennaria dioica, Arnica montana, Gentiana pneumonanthe, Thymus serpyllum, the grasses Danthonia decumbens, Deschampsia flexuosa, Nardus stricta and the shrub Calluna vulgaris was recorded. The forb A. dioica and the grass D.decumbens preferred low NH4+/NO3(-) ratios and were characterised by a negative correlation between NH4+/NO3(-) ratios and biomass and survival, whereas the grasses N. stricta and D. flexuosa showed no correlation with NH4+/NO3(-) ratios. Lime addition eliminated the negative effects of high NH4+ concentrations in deposition for A. dioica and the grass D. decumbens. The implications of these findings for heathland vegetations are discussed.     

中国对虾血细胞包掩作用的超微结构和组织化学观察

用鳗弧菌注射中国对虾,对其体内血细胞的包掩作用进行了超微结构和组织化学观察．结果表明：在注菌的对虾体内,包囊产生于多种器官和组织,在释放颗粒的过程中,半颗粒细胞和颗粒细胞的表面即发生变化,开始与周围细胞相粘接;在包囊中心是电子不透明的非结构团块;含吞噬泡的透明细胞也常被包围在包囊内,构成包囊的血细胞中细胞器趋于退化,在组织化学上表现为细胞中的ＲＮＡ大为减少,色氨酸几乎消失;然而,包囊从开始形成起,就呈Ｃｈｅｖｒｅｍｅｎｔ铁氰化钾法强阳性反应,此反应可被Ｂａｒｎｅｔ碘液法封闭．推测这一现象与黑色素生成有关．     

基于遥感和GIS的四川省草地动态变化分析

利用地理信息系统技术,在空间数据库平台的支持下,对四川省20世纪90年代中期到2000年间的草地动态变化特征、草地动态变化的背景特征及其空间分布特征进行了分析。研究表明,净增草地部分主要来源于耕地,净减草地部分的主要去向是林地。总体上,净增草地面积3812公顷,净增草地部分主要是在轻度土壤侵蚀区、中度土壤侵蚀区、剧烈土壤侵蚀区和强度土壤侵蚀区,坡度等级5、6和1的区域,环境等级5、6、4和1的区域;净减草地部分主要是在微度土壤侵蚀区和极强土壤侵蚀区,坡度等级3、2和4的区域,环境质量等级7和8的区域。净增草地的区域为川西北高原、丘陵和川西南山区,净增草地面积的前四个地州市是遂宁市、泸州市、巴中地区和广安市,净减少草地的区域为盆周山地和平原,净减少草地面积的前四个地州市是广元市、绵阳市、达川市和宜宾市。     

Social cost‐benefit analysis of intensive versus traditional shrimp farming: A case study from India

This paper attempts a social cost‐benefit analysis of intensive versus traditional shrimp farming in West Bengal, India. Using primary data, the paper shows that although intensive shrimp farming yields high returns as compared to traditional shrimp farming, when the opportunity costs and environmental costs of shrimp farming, including disease risk, are accounted for, intensive shrimp farming loses its advantage. Sensitivity analysis shows that if the expected benefits were to fall short by 15% and costs rise by a similar proportion, intensive shrimp farmers would report higher losses than traditional shrimp farmers. Large traditional shrimp farmers continue to report positive net returns. These results are also most pronounced for small and marginal intensive shrimp farmers. Further, if the probability of disease risk is also accounted for, intensive shrimp farming reports significant losses whereas traditional shrimp farming in most cases shows positive net returns. In light of the high social costs and risks, this paper questions the rationale behind promoting intensive shrimp farming, especially among small and marginal holdings, as an income‐generating activity or poverty alleviation measure in the absence of an enabling environment. It suggests that policymakers need to factor in sustainability concerns and the local context when formulating policies to promote intensive shrimp farming.     

Environmental and economic assessment of biomass sourcing from extensively cultivated buffer strips along water bodies

Buffer strips represent oblong land elements along water bodies playing an important role for the water quality management of the surface water. In the policy context buffer strips are referred to as land with defined farming restrictions aiming at protecting the water course. In the current EU agricultural policy framework the majority of the decisions regarding subsidy schemes for buffer strips is taken on the member country level, which results in great differences between the EU members with regard to this water protection measure. If incentives for farmers for establishing and maintaining buffer strips are in place, they are usually linked to the harvest ban on the buffer strip. Such protection model can be endangered by volatile and rising prices for agricultural products. However, buffer strip can represent a valuable source of different ecosystem services, including biomass provision. If harvesting under certain restrictions would be allowed, the biomass could generate additional revenue that might contribute to securing buffer strips existence and consequently maintaining their protection function.This study aimed at assessing the costs and environmental consequences of biomass mobilizing from buffer strips. It dealt with different scenarios of biomass sourcing from extensively cultivated buffer strips in the Netherlands. In 12 scenarios, the cultivation of grass or cereal mixes (including multiple harvesting or perennial cultivation) for different valorisation chains (ensiling or fodder & bedding) was assessed. Both total net cultivation costs as well as the hectare based environmental performance (using the Life Cycle Assessment methodology) were evaluated. Additionally, the environmental impact of electricity production through anaerobic digestion of biomass from buffer strips was compared with the impact from digestion of classic grass silage and the impact profile of Dutch electricity mix.The results indicate ensiling as the scenario generating more net costs and higher environmental impacts as compared to fodder & bedding. In the latter, the cereal cultivation represents a better solution from economic perspective, while grass shows lower environmental impacts. Moreover, optimizing grass cultivation through switching to perennial mode contributes to strong improvements of the economic performance and contributes to additional reduction of environmental impacts, and consequently delivers the best environmental and economic solution. Moreover, the comparison with the Dutch electricity mix shows that biomass from buffer strips, if used in anaerobic digestion, can, in terms of environmental performance, compete with classic silages and contribute to reduced environmental damage.     

Toxicity of the Mosquito Control Pesticide Scourge® to Adult and Larval Grass Shrimp (Palaemonetes pugio)

Abstract

This study investigated the toxicity of various concentrations of technical resmethrin and Scourge® on adult and larval Palaemonetes pugio, a common grass shrimp species. Two types of tests were conducted for each of the resmethrin formulations using adult and larval grass shrimp life stages, a 96-h static renewal aqueous test without sediment, and a 24-h static nonrenewal aqueous test with sediment. For resmethrin, the 96-h aqueous LC₅₀ value for adult shrimp was 0.53 μg/L (95% confidence interval (CI): 0.46–0.60 μg/L), and for larval shrimp was 0.35 μg/L (95% CI: 0.28–0.42 μg/L). In the presence of sediment, technical resmethrin produced a 24-h LC₅₀ value for adult shrimp of 5.44 μg/L (95% CI: 4.52–6.55 μg/L), and for larval shrimp of 2.15 μg/L (95% CI: 1.35–3.43 μg/L). For Scourge®, the 96-h aqueous LC₅₀ for adult shrimp was 2.08 μg/L (95% CI: 1.70–2.54 μg/L), and for larval shrimp was 0.36 μg/L (95% CI: 0.24–0.55 μg/L). The 24-h sediment test yielded an LC₅₀ value of 16.12 μg/L (95% CI: 14.79–17.57 μg/L) for adult shrimp, and 14.16 μg/L (95% CI: 12.21–16.43 μg/L) for larvae. Adjusted LC₅₀ values to reflect the 18% resmethrin concentration in Scourge® are 0.37 μg/L (adult), 0.07 μg/L (larvae) for the 96-h aqueous test, and 2.90 μg/L (adult), 2.6 μg/L (larvae) for the 24-h sediment test. Larval grass shrimp were more sensitive to technical resmethrin and Scourge® than the adult life stage. The results also demonstrate that synergized resmethrin is more toxic to P. pugio than the nonsynergized form, and that the presence of sediment decreases the toxicity of both resmethrin and Scourge®     

Governing sustainability: examining audits and compliance in a third-party-certified organic shrimp farming project in rural Indonesia

Drawing on a case study of an organic shrimp project in Indonesia, this paper provides a preliminary assessment of the use of third-party certification (TPC) to make shrimp aquaculture more environmentally sustainable in a rural setting in Indonesia. Specifically, I examine three processes of TPC: (1) the development of standards, (2) the communication of standards, and (3) the enforcement of standards. My research indicates that TPC is based on technoscientific norms and values and Western ideas of rationality. Thus, implementing TPC in rural settings in the global South may pose additional difficulties, resulting from cultural and structural differences. Consequently, TPC may not be able to be successfully implemented everywhere in the world, especially in the rural South, in its current form.     

Phalaris arundinacea L. (Reed canary grass—Gramineae) as a hydrophyte in Essex,Connecticut, USA

After the June 1982 floods in Essex, Connecticut, USA, reseeding for stabilization of exposed pond bottoms and subsequent rebuilding of a dam on the Mill Pond, Essex, CT resulted in rooted, floating aquatic mats ofPhalaris arundinacea L. This required that the site be weed harvested to restore open water. It is suggested that the species not be used to temporarily stabilize pond bottom soils because of its great and unexpected adaptability to aquatic situations.