Transport of engineered antibiotic resistance plasmids in porous media has been reported to potentially cause significant spreading of antibiotic resistance in the environment. In this work, transport of an indigenous resistance plasmid pK5 in porous media was investigated through packed column experiments. At identical ionic strengths in CaCl2 solutions, the breakthroughs of pK5 from soil columns were very close to those from quartz sand columns, indicating that transport of pK5 in quartz sand and soil was similar. A similarity in transport behavior was also found between pK5 and an engineered plasmid pBR322 that has approximately the same number of base pairs as pK5. The influence of surfactants, a major group of constituents in soil solutions, was examined using an engineered plasmid pcDNA3.1(+)/myc-His A. The impact of an anionic surfactant, sodium dodecyl sulfate (SDS), was negligible at concentrations up to 200 mg·L–1. Cetyltrimethyl ammonium bromide (CTAB), a cationic surfactant, was found to significantly enhance plasmid adsorption at high concentrations. However, at environmentally relevant concentrations (<1 mg·L–1), the effect of this surfactant was also minimal. The negligible impact of surfactants and the similarity between the transport of engineered and indigenous plasmids indicate that under environmentally relevant conditions, indigenous plasmids in soil also have the potential to transport over long distances and lead to the spreading of antibiotic resistance.
Ecology and culture comprise interacting components of landscapes. Understanding the integrative nature of the landscape is essential to establish methods for sustainable management. This research takes as a unifying theme the idea that ecological and cultural issues can be incorporated through management. As a first step in developing integrative management strategies, information must be collected that compares and contrasts ecological and cultural issues to identify their areas of intersection. Specifically how can local cultural knowledge enable water resource management that reflects cultural and ecological values? This research examines Native American cultural knowledge for setting water resource management priorities in the Wind River Indian Reservation in central Wyoming. A cross-cultural approach is adopted to assess the relationship between indigenous cultural knowledge and Euro-American perspectives through a comparative examination of the Wind River Water Code and Wyoming Water Law. This research indicates that cultural perspectives provide a rich arena in which to examine management issues. Understanding and identifying cultural practices may be an important first step in collaborative resource management between different cultural groups to prevent conflict and lengthy resolution in court. 相似文献
The terms used to describe and negotiate environmental quality are both ambiguous and value-laden. Stakeholders intimately
and actively involved in the management of forested lands were interviewed and found to use ambiguous, tautological, and value-laden
definitions of terms such as health, biodiversity, sustainability, and naturalness. This confusing language hinders public
participation efforts and produces calls to regulate and remove discretion from environmental professionals. Our data come
from in-depth interviews with environmental management professionals and other stakeholders heavily vested in negotiating
the fate of forested lands. We contend that environmental science and management will be more effective if its practitioners
embrace and make explicit these ambiguous and evaluative qualities rather than ignore and disguise them. 相似文献
Coping with ambiguities in natural resources management has become unavoidable. Ambiguity is a distinct type of uncertainty that results from the simultaneous presence of multiple valid, and sometimes conflicting, ways of framing a problem. As such, it reflects discrepancies in meanings and interpretations. Under the presence of ambiguity it is not clear what problem is to be solved, who should be involved in the decision processes or what is an appropriate course of action. Despite the extensive literature about methodologies and tools to deal with uncertainty, not much has been said about how to handle ambiguities. In this paper, we discuss the notions of framing and ambiguity, and we identify five broad strategies to handle it: rational problem solving, persuasion, dialogical learning, negotiation and opposition. We compare these approaches in terms of their assumptions, mechanisms and outcomes and illustrate each approach with a number of concrete methods. 相似文献
The international shelter response to the Jogjakarta earthquake in Indonesia in May 2006 is widely regarded as a success story, especially when compared with the response to the Indian Ocean tsunami 16 months earlier. This evaluation is largely in terms of the international aid system itself, which emphasises statistical measures of ‘success’ and internal coordination and efficiency. From the perspective of those closer to the ground, however, it was less successful, especially in terms of coordination and communication with and participation of local agencies and affected communities. This paper, by an aid worker resident in Jogjakarta and an anthropologist, examines the response from a perspective grounded both within and outside the aid system, local as well as global. It recognises the relative success of the response, but argues for an approach more grounded in local knowledge and responsive to local concerns, while also providing practical suggestions for improvement. 相似文献