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801.
Ranjan Kumar Mohapatra Pankaj Kumar Parhi Hrudayanath Thatoi 《Chemistry and Ecology》2017,33(2):114-130
Hexavalent chromium-tolerant (1500?mg/L) bacterium MW1 was isolated from harbour water of Paradip Port and evaluated for Cr(VI) reduction potential. The isolate was identified as Exiguobacterium indicum by biochemical and 16S rRNA gene sequence methods. Salt tolerance of the bacterium was evaluated in a wide range of NaCl concentrations (0.5–13%, w/v). The Cr(VI) reduction of the strain was evaluated and optimised with varied Cr(VI) concentrations (100–1000?mg/L), pH (5.0–9.0), temperature (30–40°C) and shaking velocity (100–150?rpm) in two different minimal media (M9 and Acetate). Under optimised conditions, after 192?h of incubation nearly 92%, 50% and 46% reduction in the M9 minimal medium and 91%, 47% and 40% reduction in the acetate minimal medium were observed for 100, 500 and 1000?mg/L of Cr(VI), respectively. The exponential rate equation for Cr(VI) reduction yielded higher rate constant value, that is, 1.27?×?10?2?h?1 (M9) and 1.17?×?10?2?h?1 (Acetate) in case of 100?mg/L and became lower for 500 and 1000?mg/L Cr(VI) concentrations. Further, the association of bacterial cells with reduced product was ascertained by Fourier transform infrared spectrometer, UV–Vis–DRS and field-emission scanning electron microscope–energy-dispersive X-ray analyses. The above study suggests that the higher reducing ability of the marine bacterium E. indicum MW1 will be suitable for Cr(VI) reduction from saline effluents. 相似文献
802.
采用物种敏感度排序法(SSR)对我国铅的淡水水生生物安全基准进行推导,并以太湖为例进行了流域水生生物安全基准推导。对于难以获得的本土生物毒性数据,开展了相应的毒性试验。获得了我国国家与太湖流域铅的水生生物安全基准值,基准最大浓度(CMC)分别为63.92、104.26μg·L-1,基准连续浓度(CCC)分别为1.21、4.06μg·L-1。同时,对我国主要河流以及太湖流域进行了铅的生态风险评价,联合概率曲线法显示影响5%水生生物种类的概率分别为66.22%和43.19%,熵值法则显示中国主要河流存在较大的铅暴露风险,因此,我国铅的潜在生态风险较大,主要河流与太湖流域存在铅污染问题。 相似文献
803.
为研究辽东湾表层沉积物中多环芳烃(PAHs)的来源特征,2014年5月采集了20个辽东湾海域表层沉积物样品,并利用气相色谱质谱联用仪对优先控制的16种PAHs进行测定,采用聚类分析、主成分分析-多元线性回归分析、异构体比值3种统计方法对辽东湾表层沉积物中PAHs来源特征进行了研究。结果表明,辽东湾表层沉积物中PAHs含量范围88.5~199.3 ng·g-1,平均值为(126.3±35.3)ng·g-1,其中,萘、菲和荧蒽是PAHs优势组分。通过统计分析结果表明,辽东湾北部表层沉积物中PAHs含量低于西南部,沉积物中PAHs的来源包括石油燃烧来源、煤炭、木材等生物质燃烧来源和石油来源,其中燃烧来源是主要来源,煤炭、木材等生物质燃烧来源占49.9%,石油燃烧来源和石油来源占50.1%。 相似文献
804.
Cielo Emar M. Paraoan Windell L. Rivera 《Journal of environmental science and health. Part. B》2017,52(5):306-313
Contaminated irrigation water may greatly affect not only the quality of produce but also the people exposed to it. In this study, agricultural irrigation waters in Bulacan, Philippines were assessed and found to be contaminated with Escherichia coli (E. coli) ranging from 0.58 to 4.51 log10 CFU/mL. A total of 79 isolates of E. coli were confirmed through polymerase chain reaction (PCR) amplifying the uidA gene and were tested for phenotypic resistance using 10 antimicrobials through the Kirby–Bauer disc diffusion method. Forty-six isolates (58.22%) were noted to be multidrug resistant (MDR) with high resistance rate to cephalothin, tetracycline, streptomycin, ampicillin, trimethoprim, nalidixic acid, and chloramphenicol. Moreover, this study also examined the prevalence of Class I and II integrons accounting to 67.39% and 17.39%, respectively, of the MDR E. coli strains using multiplex PCR. The results imply that the agricultural water used in Bulacan is contaminated with the fecal material of man or other animals present in the area, and the presence of MDR bacteria, which pose a potential threat to individuals in these areas, is alarming. In addition, detection of integrons could be a good marker for the identification of MDR isolates. Lastly, this study could develop strategies for the proper management of farming sites leading to the detection of food-borne pathogens and prevention of infectious diseases. 相似文献
805.
Saeed Ghavidelfar Asaad Y. Shamseldin Bruce W. Melville 《Journal of Environmental Planning and Management》2017,60(10):1809-1824
Over recent decades Auckland, New Zealand, metropolitan area has vastly expanded as a result of rapid population growth and low-density housing developments. In order to manage the uncontrolled low-density urban sprawl, Auckland Council proposed a compact city model through promoting higher density housing developments. In order to understand the implications of this transition on future residential water demand, this study first evaluated water consumption in three major housing types in Auckland including single houses, low-rise and high-rise apartments. Using the geographic information system, the water consumption information, estimated from a large sample of 60,000 dwellings across Auckland, was subsequently integrated with the Proposed Auckland Unitary Plan outlining the future housing composition over different areas in Auckland. Through developing different growth scenarios, the study showed that the housing transition from single houses to more intensified multi-unit houses cannot considerably affect the average per capita water consumption in Auckland. 相似文献
806.
ZHENMEI LÜ HANG MIN NA LI TIEJUAN SHAO YANGFANG YE 《Journal of environmental science and health. Part. B》2013,48(6):821-832
The denaturing gradient gel electrophoresis (DGGE) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). The results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of the quinclorac differed obviously with increasing incubation time within the early 8 weeks. The scale of differences in banding patterns-showed that the microbial community structures of the quinclorac-treated and non-quinclorac-treated soils were not significantly different after 21 weeks of incubation. Quantification, as demonstrated in this paper, was studied by establishing dose-response relationships. Significant pattern variations were quantified. Prominent DGGE bands were excised, cloned and sequenced to gain insight into the identities of predominant bacterial populations. The majority of DGGE band sequences were related to bacterial genera Clostridium, Sphingobacterium, Xanthomonas and Rhodococcus. 相似文献
807.
Jianshe Tang Min Zhang Guohua Cheng Ang Li Yitong Lu 《Journal of environmental science and health. Part. B》2013,48(8):707-712
Diethyl (carboxymethyl) phosphonate (DECP) was used as the hapten to develop an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for detecting organophosphorus pesticides (OPs). Conjugator of DECP with bovin serum albumin (BSA) was used as the immunogen for producing the polyclonal antibodies (PcAbs). Three antisera were obtained after the immune procedure. Characterization studies of the PcAbs indicated that the titer of antiserum-1 was highest in 3 antisera, and antiserum-1 had high affinity and specificity to the parathion, dichlorvos and pirimiphos. The IC-ELISA showed an IC50 of 0.428 μ g/mL with a detection limit of 0.0125 μ g/mL to parathion. The assay also indicated that the IC50 values of pirimiphos and dichlorvos were 0.331 μ g/mL and 1.25 μ g/mL respectively, and the detection limits of pirimiphos and dichlorvos were 0.0116 μ g/mL and 0.048 μ g/mL respectively. Recoveries of parathion, pirimiphos and dichlorvos spiked into water samples ranged from 90% to 160%. The results indicated that the ELISA could be a convenient and supplemental analytical tool for monitoring OPs residues in environmental water samples. 相似文献
808.
Dragan R. Milićević Verica B. Jurić Srđan M. Stefanović Slavica M. Vesković-Moračanin Saša D. Janković 《Journal of environmental science and health. Part. B》2013,48(8):781-787
This study was undertaken to compare two different analytical methods for the determination and confirmation of ochratoxin A (OTA) in blood serum, kidney and liver of pigs. Sample clean-up was based on liquid-liquid phase extraction. The detection of OTA was accomplished with high-performance liquid chromatography (HPLC) combined either with fluorescence detection (FL) or electro spray ionization (ESI+) tandem mass spectrometry (MS–MS). Comparative method evaluation was based on the investigation of 90 samples of blood serum, kidney and liver per animal originating from different regions of Serbia. The analytical results are discussed in view of the respective method validation data and the corresponding experimental protocols. In general, analytical data obtained with (LC–MS–MS) liquid chromatography electro spray tandem mass spectro metry detection offered comparable good results at the sub-ppb concentration level. The results indicate that the liquid chromatography electro spray tandem mass spectrometric (LC-MS/MS) method was more specific and sensitive for the analysis and confirmation of ochratoxin A in pig tissues then high pressure liquid chromatography (HPLC) method after methylation of OTA. 相似文献
809.
Achintya N. Bezbaruah Jay M. Thompson Bret J. Chisholm 《Journal of environmental science and health. Part. B》2013,48(6):518-524
Zero-valent iron nanoparticles (nZVI, diameter < 90 nm, specific surface area = 25 m2 g?1) have been used under anoxic conditions for the remediation of pesticides alachlor and atrazine in water. While alachlor (10, 20, 40 mg L?1) was reduced by 92–96% within 72 h, no degradation of atrazine was observed. The alachlor degradation reaction was found to obey first-order kinetics very closely. The reaction rate (35.5 × 10?3–43.0 × 10?3 h?1) increased with increasing alachlor concentration. The results are in conformity with other researchers who worked on these pesticides but mostly with micro ZVI and iron filings. This is for the first time that alachlor has been degraded under reductive environment using nZVI. The authors contend that nZVI may prove to be a simple method for on-site treatment of high concentration pesticide rinse water (100 mg L?1) and for use in flooring materials in pesticide filling and storage stations. 相似文献
810.
Minna Wu Xinyu Li Huiwen Zhang Yinghui Cai Chenggang Zhang 《Journal of environmental science and health. Part. B》2013,48(3):222-228
A microcosm incubation study using an aquic brown soil from northeast China (a Cambisol in the UN Food and Agriculture Organization FAO Soil Taxonomy) was conducted to examine the effects of different concentrations (0, 50, 150, and 250 mg kg?1) of methamidophos (O,S-dimethyl phosphoramidothioato) on Pseudomonas, one of the most important gram-negative bacteria in soil. Amplified ribosomal DNA restriction analysis (ARDRA) was performed to study the Pseudomonas community structure, an in vitro assay was made to test the antagonistic activity of isolated Pseudomonas strains against soil-borne Rhizoctonia solani, a major member of the pathogens highly related to soil-borne plant diseases, and special primer amplification and sequencing were performed to investigate the diversity of phlD, an essential gene in the biosynthesis of 2, 4-diacetylphloroglucinol (2, 4-DAPG), which has biocontrol activity in phlD +isolates. With exposure to increasing methamidophos concentrations, the total number of soil Pseudomonas ARDRA patterns decreased significantly, but with less change in the same treatments over 1, 3, and 5 weeks of incubation. The number of isolated Pseudomonas strains with antagonistic activity against R. solani as well as the diversity and appearance frequency of the strains' phlD gene also decreased with increasing concentrations of methamidophos, especially at high methamidophos concentrations. Applying methamidophos could increase the risk of soil-borne plant diseases by decreasing the diversity of the soil Pseudomonas community and the amount of R. solani antagonists, particularly those with the phlD gene. 相似文献