A chromosome 21-specific cosmid cocktail for the detection of chromosome 21 aberrations in interphase nuclei

Fluorescent in situ hybridization (FISH) with a 21q11-specific probe (CB21c1) consisting of three non-overlapping cosmids has been applied to interphase amniocytes of pregnancies at increased risk for fetal aneuploidy (N = 78) and to interphase lymphocytes, cultured and uncultured, of patients referred for Down syndrome (N = 19 and 28, respectively). In the uncultured amniocytes, six chromosome aberrations were detected: three cases of trisomy 21, a triploidy, a de novo 46,XX,t(21q21q), and a mosaic 46,XY/47,XY,+dic(21)(q11)/48,XY,+dic(21)(q11), +del(21)(q11). In 15 cultured and 20 uncultured blood samples, FISH correctly diagnosed trisomy 21 (full or mosaic) at the interphase level, which was confirmed in all cases by subsequent karyotyping. Because of specific and strong signals in interphase nuclei, CB21c1 appears to be a useful tool for the rapid detection of chromosome 21 abnormalities.     

有机污染物多介质环境的稳态非平衡模型

在稳态平衡模型的基础上，研究了有机污染物在多介质环境的稳态非平衡模型，并针对ＤＤＴ、１，２，４，－ＴＣＢ、ＨＣＢ、ＰＣＢ和ＤＭＰ５种化合物进行了计算。结果表明，该模型只须通过污染物在水中的浓度以及该化合物的各种物理化学性质和环境参数就可计算环境各相中污染物的浓度、逸度和质量分布，从而预测污染物在多介质环境中的归趋。     

Effects of nutrients on Microcystis growth more easily forming bloom

Different nutrient media experimentally were N, P and Fe-limited conditions and a serial of diluted BGll media. The cell change of morphology and life history, cell number, cell color and cell area of Microcystis were analyzed quantitatively. First, the effects of nitrogen, phosphorus and iron depletion were distinctively different. Phosphorus and iron depletion caused more special division cells,slowly growth increasing, the easier change of bigger cell area. Second, the nitrogen and iron depletion could make the color of alga from green to brown. Finally, according to the resource competition and Monod equation, Microcystis kinetics of phosphorus and iron were also examined. Ks and μmax of phosphorus absorption were 0.0352 μmol/L, 0.493 d^-1 respectively; iron absorption: 0.00323 μmol/L, 0.483d^-1. In a word, some evidences of the Microcystis bloom dominance in certain nutrient conditions were indicated in the experiments. The dominances were determined as the reviving under the adverse circumstances through the special division, the various nitrogen resources,and the lower kinetics of phosphorus and iron than that of most of other algae. The conclusions provided the scientific basis for preventing and managing Microcystis bloom in freshwater.     

TC11钛合金盘形件锻造过程的有限元模拟

在建立动态接触边界条件的通用处理方法的基础上,采用刚粘塑性有限元法模拟了TC11钛合金盘形件的锻造过程。     

Congenital heart disease and aneuploidy

Congenital heart disease (CHD) is one of the commonest prenatal diagnoses made on routine ultrasound screening. Overall, up to 33% of CHD are associated with fetal aneuploidy. However, some specific cardiac lesions have a significantly greater association with particular chromosomal abnormalities. The majority of fetuses with CHD and aneuploidy also have extra-cardiac anomalies and are best managed by a multidisciplinary team where the management and prognosis of the cardiac defect can be discussed in the context of the baby as a whole. It is therefore important for clinicians involved in the management of fetuses with CHD to be aware of the association of aneuploidy as well as the prognosis and management of these cases, so that they can appropriately counsel the parents. In this chapter, we review the frequency and types of aneuploidy associated with the commonly diagnosed CHD and discuss their management. Copyright © 2004 John Wiley & Sons, Ltd.     

北京大气中CFC-11的浓度观测与变化趋势

近几年大气中CFC的浓度在人类活动的影响下发生了迅速变化,考虑到CFC浓度变化对平流层臭氧和全球变暖的影响,采用两步深冷冻浓缩自动进样系统,配以气相色谱/质谱联机对北京大气中的CFC-11进行了连续观测.结果表明,1999～2003年CFC-11的浓度季节变化均呈单峰形态,峰值出现在7～8月,月平均浓度最高值为1149.5±531.9×10^-12(体积分数);谷值出现在春季的3～5月份,月平均浓度最低值为487.5±131.5×10^-12(体积分数);北京大气中CF-11年平均浓度在观测时间段内呈先上升后下降的趋势,其中1995～1998年增长较快,平均增长率为17.9%,1999年后呈缓慢下降趋势,平均下降率为10.7%,平均浓度是Mauna Loa全球基准观测站观测到大气本底CFC-11浓度的3～5倍.     

基于MIKE耦合模型的入河污染模拟与控制效能研究

为解决沙河水库流域河道水质差、水环境退化等问题,以主要超标污染物CODCr、NH3-N和TP为指标,应用MIKE 11、MIKE SHE及ECO Lab构建流域水文水动力及水质耦合模型,探究污染物的迁移转化规律,并进一步评估不同控制措施下的河道水质改善效能.研究表明:耦合模型在研究区具有良好的适应性,水动力模拟精度良好...