Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates

Nanotechnology has attracted a great interest in recent years due to its expected impact on many areas such as energy, medicine, electronics, and space industries. This review provides the state-of-art knowledge on the synthesis of nanoparticles by microorganisms including bacteria, fungi, actinomycetes, and yeast, and their effect on microbiological processes. The available microbes and their predicted nanoparticle biosynthesis mechanism, the conditions to control the size/shape and monodispersity of particles, and microbiological reaction rate enhancement using nanoparticles as catalysts are presented. The current limitations and future scope for specific research are also discussed.     

Microbial biodegradation of plastics: Challenges,opportunities, and a critical perspective

● Health hazards of plastic waste on environment are discussed. ● Microbial species involved in biodegradation of plastics are being reviewed. ● Enzymatic biodegradation mechanism of plastics is outlined. ● Analytical techniques to evaluate the plastic biodegradation are presented. The abundance of synthetic polymers has increased due to their uncontrolled utilization and disposal in the environment. The recalcitrant nature of plastics leads to accumulation and saturation in the environment, which is a matter of great concern. An exponential rise has been reported in plastic pollution during the corona pandemic because of PPE kits, gloves, and face masks made up of single-use plastics. The physicochemical methods have been employed to degrade synthetic polymers, but these methods have limited efficiency and cause the release of hazardous metabolites or by-products in the environment. Microbial species, isolated from landfills and dumpsites, have utilized plastics as the sole source of carbon, energy, and biomass production. The involvement of microbial strains in plastic degradation is evident as a substantial amount of mineralization has been observed. However, the complete removal of plastic could not be achieved, but it is still effective compared to the pre-existing traditional methods. Therefore, microbial species and the enzymes involved in plastic waste degradation could be utilized as eco-friendly alternatives. Thus, microbial biodegradation approaches have a profound scope to cope with the plastic waste problem in a cost-effective and environmental-friendly manner. Further, microbial degradation can be optimized and combined with physicochemical methods to achieve substantial results. This review summarizes the different microbial species, their genes, biochemical pathways, and enzymes involved in plastic biodegradation.     

应用有效微生物群(EM)处理富营养化源水试验研究

初试条件下系统地考察了有效微生物群(EM)处理南水塘藻型富营养化源水效果。结果表明:在容器中按V(EM)∶V(源水)为1∶10000的比例投加EM菌液并辅以低速间歇式曝气处理8～9d,藻类生物量得到明显控制,水样叶绿素a,TN,TP及CODCr去除率分别达90 49%,45 25%,55 48%及82 37%,出水水质接近国家地表Ⅳ类水质标准。处理过程中,水样pH与电导率有明显的变化规律,应属2个独立的因子体系;EM微生物可改变源水氮磷营养盐水平及藻类生物量与氮 磷质量分数的相关性,成为影响或抑制藻类生长的主导因子。      

芳香类污染物的厌氧生物降解研究

综述了国内外芳香类污染物厌氧降解的研究进展,详细阐述了含氮芳香类、氯代芳香类和酚类污染物厌氧降解的微生物类群与机理.提出了芳香类污染物生物降解今后发展方向的重点是共基质条件下生物降解性研究、厌氧-好氧条件下的生物降解性研究、高效工程菌和固定化细胞技术应用研究、构建基因工程菌和遗传学研究等技术领域.     

马兰黄土中与三氮有关的微生物的分布及其反硝化能力

选择关中盆地马兰黄土为研究对象,采用平板菌落计数法研究了与三氮转化有关的微生物在0～9 m剖面上的垂直分布.结果表明,剖面上氨化细菌、硝化细菌、反硝化细菌、固氮菌分别为克土106～1010、101～103、103～108、103～105个.在试验区剖面7 m处的土壤中存在着反硝化菌富集区,数量达克土108个.对此层位土壤进行了反硝化能力实验,结果表明,马兰黄土反硝化作用最适宜温度为22 ℃和37 ℃,碳源的存在可大大提高反硝化速率,但改变碳氮比对反硝化速率影响不大.在15 ℃、无外界供给碳源、硝酸根初始质量浓度为56 mg/L情况下,反硝化菌对硝酸根的降解作用符合方程y=-2.682 8x 60.979,回归系数R=0.960 9.该反硝化菌富集区的存在为研究包气带中三氮转化提供了重要信息.     

有效微生物技术在城市环境治理领域中的应用

随着经济的快速发展,人口数量急剧增长,我国城市水污染及生活垃圾的安全处置问题日益严重,因此找到一种简单、经济的处理方法是目前迫切需要解决的问题。介绍了有效微生物的种群特点以及处理污水的机理,阐述了有效微生物对污水处理效果的影响因素,综述了有效微生物技术在污水处理、河湖富营养化治理、生活垃圾处置等方面的研究现状,并指出了该技术在城市环境治理领域的发展趋势和研究方向。     

Microbial Removal of Arsenic

Bangladesh is currently the subject of the world's largest mass arsenic poisoning in history. Groundwater throughout Bangladesh and West Bengal is contaminated with naturally occurring arsenic from the alluvial and deltaic sediments that form the region's aquifers. It has been estimated that 75 million people are at risk of developing health effects associated with the ingestion of arsenic. This project focuses on the use of microorganisms such as bacteria and algae to remove arsenic from water. Arsenic in the arsenite form was used in the studies. Experiments were conducted with a common alga and wastewater bacteria. A common green algae Scenedesmus abundans was used for determining arsenic uptake in batch experiments. Results of the experiments indicated that the algae biosorption could be modeled by the conventional Langmuir isotherm model. Algae morphology studies indicated that the algae cells were impacted due to the presence of arsenic as evidenced by clumping or loss of cell clusters. The wastewater bacteria also were capable of high percent of arsenic removal. Results indicate that microbial uptake of arsenic may be a viable method of pretreatment of arsenic contaminated water. However algae and sludge disposal would pose a problem and will have to be dealt with accordingly.     

多级曝气-固定化优势微生物强化固定生物膜处理工艺的应用

广州白云山一峰污水处理工程采用了新型污水处理工艺-多级曝气-微生物制剂强化固定生物膜处理工艺,对该工艺的特点及设计体会进行了讨论。设计进水COD、BOD5、SS、NH3-N、TP、动植物油为400～600mg/L、300～400mg/L、250～400mg/L、20～30mg/L、3～5mg/L、70～100 mg/L,出水为90mg/L、20mg/L、10mg/L、10mg/L、0.5mg/L、10 mg/L。实际运行结果表明：主要出水指标COD、BOD5、SS、NH3-N、TP、动植物油的去除率分别可达到88.1%、94.4%、97.9%、88.6%、94.6%、91.5%。该工艺具有占地少、建设成本低、结构简单、施工容易、能耗低、噪声低、运行可靠、稳定达标、剩余污泥极少、运行成本底、维护简便等优点。     

利用微生物除臭技术研究与应用

简述了微生物在降解生产和生活中臭气方面的研究与应用,特别着重介绍了EM复合菌群在除臭方面的卓越效果,表明了生物除臭与物理、化学方法除臭相比具有彻底性、实用性与经济性的特点,在环境保护与资源的重复利用方面有着巨大的应用潜力。     

Role of specific microbial communities in the bioavailability of iron in Doñana National Park

The Doñana National Park contains highly complex microbial communities that play critical roles in the bioavailability and biogeochemical cycling of elements. In this study, we analyzed the formation of Fe-rich films on the surface of shallow waters at Doñana National Park as a model for future applications in bioremediation. Phytoplankton composition and abundance, physicochemical measurements, culture enrichments, and molecular detection of microorganisms were determined during this study. Natural samples were incubated in the laboratory and enrichments producing similar phenomena were obtained. Bacteria belonging to the genera Enterobacter were the major participants in surface Fe-film formation in laboratory enrichments. Film formation was dependent on wind exposure and only those sites protected from the direct influence of wind developed these films. A higher concentration of euglenophytes was found in the proximity of film-covered waters. Available iron (Fe²⁺) and total Fe concentrations were high (18.8 and 46.6 mg/L, respectively) underneath the surface film, and higher than in film-free waters and pore-sediment water. The concentration of phosphate and its availability were higher in the iron-rich film sediment, as 61% of total P was bound to iron oxy-hydroxides. Our results provide evidence of the link between P and Fe biogeochemical cycling in aquatic systems on sandy soils at the Doñana National Park. In addition, they provide significant insights into the bioavailability of these elements with potential interest in the role of microorganisms for metal sequestration in natural environments and utilization in bioremediation processes.