Mitochondrial electron transport chain is involved in microcystin-RR induced tobacco BY-2 cells apoptosis

Microcystin-RR （MC-RR） has been suggested to induce apoptosis in tobacco BY-2 cells through mitochondrial dysfunction including the loss of mitochondrial membrane potential . TO further elucidate the mechanisms involved in MC-RR induced apoptosis in tobacco BY-2 cells, we have investigated the role of mitochondrial electron transport chain （ETC） as a potential source for reactive oxygen species （ROS）. Tobacco BY-2 cells after exposure to MC-RR （60 mg/L） displayed apoptotic changes in association with an increased production of ROS and loss of Am. All of these adverse effects were significantly attenuated by ETC inhibitors including Rotenone （2 μmol/L, complex I inhibitor） and antimycin A （0.01 μmol/L, complex III inhibitor）, but not by thenoyltrifluoroacetone （S μmol/L, complex Ⅱinhibitor）. These results suggest that rnitochondrial ETC plays a key role in mediating MC-RR induced apoptosis in tobacco BY-2 cells through an increased mitochondrial production of ROS.     

Visitor Perceptions and the Shifting Social Carrying Capacity of South Sinai’s Coral Reefs

To investigate how the perceptions and behaviour of visitors to coral reefs are influenced by their prior experience and knowledge of marine life, a questionnaire-based study was undertaken at sites in the Ras Mohammed National Park and at Sharm El Sheikh, South Sinai, Egypt. It was evident that over the 10–20 years during which these reefs have deteriorated (mainly due to reef-flat trampling), there have been interrelated shifts in the nature of visitors making use of them. First, there has been a shift from experienced divers and snorkellers to inexperienced snorkellers and non-snorkellers with a poorer knowledge of reef biology. Second, there has been a shift in the predominant nationalities of visitors, from German and British, through Italian, to Russian. More recent user groups both stated and showed that they had less experience of snorkelling; they also showed less knowledge of marine life and less interest in learning about it. Visitor perceptions of both the state of the marine life on the reefs and the acceptability of current visitor numbers also varied between groups. More recent visitor groups and visitors with less knowledge were more satisfied with reef health. In general, however, visitor perceptions of reef health did not correlate well with actual reef conditions, probably because more experienced visitors preferred less impacted sites with which they were nevertheless less satisfied than inexperienced visitors at heavily impacted sites. More recent visitor groups were also less bothered by crowding on the shore or in the water. Consequently, the apparent “social carrying capacity” of sites seems to be increasing to a level well above the likely “ecological carrying capacity”.     

Upscaling heterogeneity in aquifer reactivity via exposure-time concept: forward model

Reactive properties of aquifer solid phase materials play an important role in solute fate and transport in the natural subsurface on time scales ranging from years in contaminant remediation to millennia in dynamics of aqueous geochemistry. Quantitative tools for dealing with the impact of natural heterogeneity in solid phase reactivity on solute fate and transport are limited. Here we describe the use of a structural variable to keep track of solute flux exposure to reactive surfaces. With this approach, we develop a non-reactive tracer model that is useful for determining the signature of multi-scale reactive solid heterogeneity in terms of solute flux distributions at the field scale, given realizations of three-dimensional reactive site density fields. First, a governing Eulerian equation for the non-reactive tracer model is determined by an upscaling technique in which it is found that the exposure time of solution to reactive surface areas evolves via both a macroscopic velocity and a macroscopic dispersion in the artificial dimension of exposure time. Second, we focus on the Lagrangian approach in the context of a streamtube ensemble and demonstrate the use of the distribution of solute flux over the exposure time dimension in modeling two-dimensional transport of a solute undergoing simplified linear reversible reactions, in hypothetical conditions following prior laboratory experiments. The distribution of solute flux over exposure time in a given case is a signature of the impact of heterogeneous aquifer reactivity coupled with a particular physical heterogeneity, boundary conditions, and hydraulic gradient. Rigorous application of this approach in a simulation sense is limited here to linear kinetically controlled reactions.     

Shorter fries? An alternative policy to support a reduction of nitrogen contamination from agricultural crop production

Feeding the growing population of the world poses significant policy challenges for the sustainability of global ecosystems. A prime example is the degradation of water quality due to the growing imbalance in the terrestrial nitrogen (N) cycle linked to increasing production of reactive N (Nr). Environmental impacts such as groundwater quality degradation and eutrophication of coastal estuaries tend to be local in nature but may be closely connected to global economic factors. Environmental accounting of the N fluxes entering, leaving or remaining in Prince Edward Island (PEI), a small agricultural region, demonstrate the importance of a single industry (potato production) in controlling the local N cycle. The resulting burden of Nr has its most profound effect on groundwater, the sole source of drinking water and the primary pathway of N to the Province's economically and ecologically important estuaries. At the same time, agriculture is a vital part of the local economy, and regulators are faced with the challenge of meeting environmental goals and still maintaining an industry that is competitive and responsive to global market trends. New, innovative policy alternatives are needed to foster more effective implementation of sustainable agricultural practices. An approach that focuses on influencing consumer choices toward more environmentally responsible production practices at the point of origin may help remove some of the important, non-technical barriers to sustainable food production practices. In practice, a system that documents the environmental performance throughout the full supply chain from producer to retailer would have to be implemented.     

Coupled modeling of cement/claystone interactions and radionuclide migration

The interactions between cement and a clayey host-rock of an underground repository for intermediate-level radioactive waste are studied with the reactive transport code HYTEC for supporting performance assessment. Care is taken in using relevant time scales (100,000 years) and dimensions. Based on a literature review, three hypotheses are considered with respect to the mineralogical composition of the claystone and the neo-formed phases. In the long term, the pH is buffered for all hypotheses and important mineral transformations occur both in cement and the host-rock. The destruction of the primary minerals is localized close to the cement/claystone interface and is characterized by the precipitation of secondary phases with retention properties (illite, zeolite). However, beyond the zone of intense mineral transformations, the pore water chemistry is also disturbed over a dozen meters due to an attenuated but continuous flux of hydroxyl, potassium and calcium ions. Four interdependent mechanisms control the profile in the whole system: diffusion of the alkaline plume, mineralogical buffering, ion exchange and clogging of the pore space at the cement/claystone interface. The migration of a selected group of radionuclides (Cs, Ra, Tc and U) is explicitly integrated in the simulations of the strongly coupled system. Theoretical profiles of distribution coefficient (Kd) and solubility limit values are derived from the simulations, and their sensitivity with respect to the system evolution is estimated.     

Proliferating Principles; Or How to Sup with the Devil without Getting Eaten

The international humanitarian system is seen by many to be in crisis. The reasons for this are complex but include the perception held by many that there is an increasing ambivalence on the part of powerful states to invest military, political and financial resources in upholding humanitarian principles, and a growing feeling that much humanitarian action both ignores human rights issues and can prolong conflict. As a result, much attention has focused on the notion of 'humanitarian principles' and there has been a proliferation of statements and initiatives on this topic in recent years.     

FLOOD WARNINGS AND FLOOD RESPONSES FOR THE RED RIVER OF THE NORTH1

ABSTRACT The flooding conditions in the basin of the Red River of the North are reviewed in terms of the accuracy of the flood forecasts and the response of both the floodplain occupants and government agencies to these forecasts. The flood prediction methods in Canada and the United States are compared. The accuracy of these prediction measures for the major floods in recent history is reviewed. The differences between the way in which the American and Canadian authorities approach the flood emergencies are outlined. The accuracy of the forecasts are plotted against a number of parameters which reflect the efficiency of the flood fighting measures initiated by those flood forecasts. The significant features of these plots are discussed.     

活性粉末混凝土(RPC)的断裂力学性能

在试验及观察的基础上,对活性粉末混凝土的断裂过程进行定性分析,给出活性粉末混凝土的裂纹发展原理及钢纤维的增韧机理,并分析其不同于普通混凝土的两个基本断裂特征。介绍了利用缺口梁三点弯曲试验确定混凝土拉伸软化本构方程的方法。基于Hillerborg模型,提出活性粉末混凝土的拉伸软化模型,根据三点弯曲断裂试验结果,确定出活性粉末混凝土的双线性拉伸软化曲线中的具体参数。据该软化曲线公式,计算出的活性粉末混凝土断裂能同试验得出的值相比,误差仅为0.9%。笔者得出的双线性软化曲线能较精确地反映活性粉末混凝土材料的断裂特性,可为今后进行RPC裂缝的分析和预测提供定量计算的依据,对于研究活性粉末混凝土的断裂能、尺寸效应等问题也很具意义。     

秦皇岛市海洋生态环境保护与建设探讨

本文通过对秦皇岛市海洋生态环境质量的调查分析,提出了影响生态环境 的主要问题,进而对该海域的生态环境保护与生态建设提出了切实可行的对策。     

磁性CuS/γ-Fe2O3复合材料光催化处理染料废水

以氯化铜、纳米γ-Fe₂O₃和硫脲为原材料,乙二醇为溶剂,采用溶剂热法制备了磁性CuS/γ-Fe₂O₃复合光催化剂。考察了该光催化剂对刚果红染料废水的处理效果。在m（CuS）:m（γ-Fe₂O₃）=2:1、刚果红初始质量浓度为10 mg/L、光催化剂投加量为0.6 g/L的最佳工艺条件下,刚果红去除率达96.51%。阴离子Cl^-、NO₃^- 及SO₄^2-对该光催化剂的催化活性具有促进作用,其中SO₄^2–的促进作用最显著。该光催化剂具有较好的活性稳定性,重复使用6次后刚果红去除率仍高达90.50 %。