Assisted phytoremediation of mixed metal(loid)-polluted pyrite waste: effects of foliar and substrate IBA application on fodder radish

Exogenous application of plant-growth promoting substances may potentially improve phytoremediation of metal-polluted substrates by increasing shoot and root growth. In a pot-based study, fodder radish (Raphanus sativus L. var. oleiformis Pers.) was grown in As-Zn-Cu-Co-Pb-contaminated pyrite waste, and treated with indolebutyric acid (IBA) either by foliar spraying (10 mg L⁻¹), or by direct application of IBA to the substrate (0.1 and 1 mg kg⁻¹) in association, or not, with foliar spraying. With the exception of foliar spraying, IBA reduced above-ground biomass, whilst direct application of IBA to the substrate surface reduced root biomass (−59%). Trace element concentrations were generally increased, but removals (mg per plant) greatly reduced with IBA application, together with greater metal leaching from the substrate. It is concluded that, in our case, IBA had a negative effect on plant growth and phytoextraction of trace elements, possibly due to unsuitable root indoleacetic acid concentration following soil IBA application, the direct chelating effect of IBA and the low microbial activity in the pyrite waste affecting its breakdown.     

Assessing urban habitat quality based on specific leaf area and stomatal characteristics of Plantago lanceolata L

This study has evaluated urban habitat quality by studying specific leaf area (SLA) and stomatal characteristics of the common herb Plantago lanceolata L. SLA and stomatal density, pore surface and resistance were measured at 169 locations in the city of Gent (Belgium), distributed over four land use classes, i.e., sub-urban green, urban green, urban and industry. SLA and stomatal density significantly increased from sub-urban green towards more urbanised land use classes, while the reverse was observed for stomatal pore surface. Stomatal resistance increased in the urban and industrial land use class in comparison with the (sub-) urban green, but differences between land use classes were less pronounced. Spatial distribution maps for these leaf characteristics showed a high spatial variation, related to differences in habitat quality within the city. Hence, stomatal density and stomatal pore surface are assumed to be potentially good bio-indicators for urban habitat quality.     

Effects and mechanisms of the combined pollution of lanthanum and acid rain on the root phenotype of soybean seedlings

Rare earth pollution and acid rain pollution are both important environmental issues worldwide. In regions which simultaneously occur, the combined pollution of rare earth and acid rain becomes a new environmental issue, and the relevant research is rarely reported. Accordingly, we investigated the combined effects and mechanisms of lanthanum ion (La³⁺) and acid rain on the root phenotype of soybean seedlings. The combined pollution of low-concentration La³⁺ and acid rain exerted deleterious effects on the phenotype and growth of roots, which were aggravated by the combined pollution of high-concentration La³⁺ and acid rain. The deleterious effects of the combined pollution were stronger than those of single La³⁺ or acid rain pollution. These stronger deleterious effects on the root phenotype and growth of roots were due to the increased disturbance of absorption and utilization of mineral nutrients in roots.     

Ovarian activity of Drosophila melanogaster meigen (diptera), during a chronic intoxication with four fungicides: Anatomical and cytological study

Abstract

Two types of reactions were observed on the alteration of Drosophila vitellogenesis by the four fungicides used in this study. Dithane®M45 resulted in stimulation associated with egg retention. However, the other three fungicides (Benlate®, Bouillie bordelaise® and Euparène®) resulted in inhibition to a varying degree. Although the inhibition was comparatively limited due to Benlate® it induced an egg retention. The inhibition was very high due to Bouillie bordelaise® and Euparène®. With Bouillie bordelaise® an egg retention occured together with the reduction of vitellogenesis and caused an increase in the rate of the follicle resorption. The latter depended on the duration of treatment. With Euparène®, no egg retention was observed and the toxicity was only noticed on vitellogenesis.     

Enhanced nitrogen deposition exacerbates the negative effect of increasing background ozone in Dactylis glomerata, but not Ranunculus acris

The combined impacts of simulated increased nitrogen (N) deposition (75 kg N ha⁻¹ yr⁻¹) and increasing background ozone (O₃) were studied using two mesotrophic grassland species (Dactylis glomerata and Ranunculus acris) in solardomes, by means of eight O₃ treatments ranging from 15.5 ppb to 92.7 ppb (24 h average mean). A-C_i curves were constructed for each species to gauge effects on photosynthetic efficiency and capacity, and effects on biomass partitioning were determined after 14 weeks. Increasing the background concentration of O₃ reduced the healthy above ground and root biomass of both species, and increased senesced biomass. N fertilisation increased biomass production in D. glomerata, and a significantly greater than additive effect of O₃ and N on root biomass was evident. In contrast, R. acris biomass was not affected by high N. The study shows the combined effects of these pollutants have differential implications for carbon allocation patterns in common grassland species.     

Lysimeter Soil Retriever (LSR)—An Application of a New Technique for Retrieving Soils from Lysimeters

In Europe more than 2,500 lysimeters operated by research institutes and industry (Lanthaler 2005). Originally lysimeters were built for investigations of soil water and solutes, nutrient leaching and pesticide degradation (see e.g. Winton and Weber 1996). Currently lysimeters additionally used as a tool for investigations on biological processes, and structural changes of plants, including root distribution, and enzyme activities etc. (see e.g. Dizer et al. 2002; Schloter et al. 2005).     

Allocation plasticity and plant-metal partitioning: meta-analytical perspectives in phytoremediation

In this meta-analysis of plant growth and metal uptake parameters, we selected 19 studies of heavy metal (HM) phytoremediation to evaluate trends of allocation plasticity and plant-metal partitioning in roots relative to shoots. We calculated indexes of biomass allocation and metal distribution for numerous metals and plant species among four families of interest for phytoremediation purposes (e.g. Brassicaceae, Fabaceae, Poaceae, and Solanaceae). We determined that plants shift their biomass and distribute metals more to roots than shoots possibly to circumvent the challenges of increasing soil-HM conditions. Although this shift is viewed as a stress-avoidance strategy complementing intrinsic stress-tolerance, our findings indicate that plants express different levels of allocation plasticity and metal partitioning depending on their overall growth strategy and status as ‘fast-grower’ or ‘slow-grower’ species. Accordingly, we propose a conceptual model of allocation plasticity and plant-metal partitioning comparing ‘fast-grower’ and ‘slow-grower’ strategies and outlining applications for remediation practices.     

The nitrate leached below maize root zone is available for deep-rooted wheat in winter wheat-summer maize rotation in the North China Plain

In winter wheat (Triticum aestivum L.)-summer maize (Zea mays L.) rotation system in the North China Plain, maize roots do not extend beyond 1.2 m in the vertical soil profile, but wheat roots can reach up to 2.0 m. Increases in soil nitrate content at maize harvest and significant reductions after winter wheat harvest were observed in the 1.4-2.0 m depth under field conditions. The recovery of 15N isotope (calcium nitrate) from various (1.0, 1.2, 1.4, 1.6, 1.8 and 2.0 m) soil depths showed that deep-rooting winter wheat could use soil nitrate up to the 2.0 m depth. This accounted partially, for the reduced nitrate in the 1.4-2.0 m depth of the soil after harvest of wheat in the rotation system.     

Improving safety and availability of complex systems using a risk-based failure assessment approach

This paper presents a structured risk-based failure assessment (RBFA) approach, which provides a complete solution to avoid repeated and potential failures to improve overall plant safety and availability. Technological advancements and high product demand have encouraged designers to design mega-capacity systems to enhance system utilization and improve revenues. However, these benefits make the systems more complex and thus prone to unnoticed failure. It is an overwhelming task to address all the failures due to the limited resources and time constraints. This leads to substandard and poor quality failure assessments, which cause repeated failures. To address this common industry concern, a four phase RBFA framework is proposed which is not limited to the identification of root cause(s) but also includes other actions such as failure monitoring. The four phases include the plan phase, the assessment phase, the analysis phase and the implementation-tracking phase. These phases cover identification of failure, failure analysis, root cause(s) analysis, and failure monitoring. In this paper, the applicability and advantages of the proposed approach are examined through two real case studies pertaining to bearing failure and drive coupling failure. By implementing the proposed approach, significant improvements have been experienced in the system availability in both the cases.