水稻土中五氯酚的降解转化动态及其对微生物群落的影响

采用室内培养实验,研究厌氧条件下水稻土中五氯酚(Pentachlorophenol,PCP)的还原转化与微生物群落组成变化。结果表明,室内培养实验条件下,PCP在水稻土样品中降解比较迅速,在反应17 d时,实验添加的PCP能够完全被还原转化。高通量测序结果显示PCP的添加明显改变了水稻土壤的微生物群落结构,其微生物多样性显著下降,在PCP降解完之后逐渐恢复。原始土壤以及添加PCP的土壤样品中其优势微生物主要分布在变形菌门(Proteobacteria)。PCP添加刺激了水稻土中伯克氏菌科(Burkholderiaceae)、丛毛单胞菌科(Comamonadaceae)、地杆菌科(Geobacteraceae)、红环菌科(Rhodocyclaceae)和假单胞菌科(Pseudomonadaceae)等脱氯相关的微生物菌群繁殖,成为PCP降解过程中的优势菌群,有利于PCP还原降解。本研究结果可为水稻土中有机氯农药污染物的微生物降解脱毒提供理论依据。     

红壤丘陵区典型流域地表水水环境特征研究——以浏阳河流域为例

选取浏阳河流域为例,根据近十年河流断面监测数据,采用改进的综合污染指数法来评价水质污染程度,研究了该红壤丘陵区典型河流水质的时空变化特征,并结合土地利用和土壤特征等分析地表水质变化原因。结果表明,改进的综合污染指数法有较好的适用性;从时间特征上看,由于面源污染加剧,使得浏阳河近十年的水质污染呈增长趋势;从空间特征上看,浏阳河从上游到下游,河流污染呈增长趋势,上游水质较好,中游表现为重金属铅和汞的污染较大,而下游则是氨氮污染加剧。     

Microbiological characteristics in a zero-valent iron reactive barrier

Zero-valent iron (Fe⁰)-based permeable reactive barriertreatment has been generating great interest for passivegroundwater remediation, yet few studies have paid particularattention to the microbial activity and characteristics withinand in the vicinity of the Fe⁰-barrier matrix. The presentstudy was undertaken to evaluate the microbial population andcommunity composition in the reducing zone of influence byFe⁰ corrosion in the barrier at the Oak Ridge Y-12 Plantsite. Both phospholipid fatty acids and DNA analyses were usedto determine the total microbial population and microbialfunctional groups, including sulfate-reducing bacteria,denitrifying bacteria, and methanogens, in groundwater andsoil/iron core samples. A diverse microbial community wasidentified in the strongly reducing Fe⁰ environment despitea relatively high pH condition within the Fe⁰ barrier (up topH 10). In comparison with those found in the backgroundsoil/groundwater samples, the enhanced microbial populationranged from 1 to 3 orders of magnitude and appeared to increase from upgradient of the barrier to downgradient soil. Inaddition, microbial community composition appeared to change overtime, and the bacterial types of microorganismsincreased consistently as the barrier aged. DNA analysisindicated the presence of sulfate-reducing and denitrifyingbacteria in the barrier and its surrounding soil. However, theactivity of methanogens was found to be relatively low,presumably as a result of the competition by sulfate/metal-reducing bacteria and denitrifying bacteria because of the unlimited availability of sulfate and nitrate in the site groundwater. Results of this study provide evidenceof a diverse microbial population within and in the vicinity ofthe iron barrier, although the important roles of microbial activity, either beneficially or detrimentally, on the longevityand enduring efficiency of the Fe⁰ barriers are yet to be evaluated.     

How and why is Aquatic Quality Changing at Nahanni National Park Reserve, nwt, Canada?

Nahanni National Park Reserve is located at southwestern NWT-Yukon border. One of the first UNESCO World Heritage sites, Nahanni lies within Taiga Cordillera and Taiga Shield Ecozones. Base and precious metal mining occurred upstream of Nahanni prior to park establishment. Nahanni waters, sediments, fish, and caribou have naturally elevated metals levels. Baseline water, sediment and fish tissue quality data were collected and analyzed throughout Nahanni during 1988–91 and 1992–97. These two programs characterized how aquatic quality variables are naturally varying in space and time, affected by geology, stream flow, seasonality, and extreme meteorological and geological events. Possible anthropogenic causes of aquatic quality change were examined. Measured values were compared to existing Guidelines and site-specific objectives were established.     

中国水环境的NPSP问题及对策

介绍了我国水环境中的非点源污染（ＮｏｎｐｏｉｎｔＳｏｕｒｃｅｐｏｌｌｕｔｉｏｎ，缩写为ＮＰＳＰ）的情况，强调农业生产是最主要的非点源污染。同时，针对我国水污染防治的政策目标，提出一些建议。     

中国北方干旱地区沙尘矿物特征研究

为了开展对我国沙暴传输规律的研究,了解我国北方干旱地区沙尘矿物特征有重要意义。本文对我国新疆塔克拉玛干沙漠区,内蒙腾格里沙漠区,贺兰山及银川等处的沙尘样品用X射线粉末衍射法进行矿物分析鉴定,并用原子吸收法作了特征元素的分析对比,根据矿物的X射线粉末衍射结果,结合元素分析,对沙漠源区及其以东地区沙尘矿物特征作了比较,判析了两个源区的矿物组成,讨论了它们的传输过程。     

Assessment of the Heavy Metal Pollution Effects on the Soil Respiration in the Baix Llobregat (Catalonia, NE Spain)

A main goal of investigations is to determine could a soilrespiration be an indicator of the soil pollution. In this case a measured levelof the soil oxygen consumption depends of its pollution. It alsomeans that the pollution reduces biological processes in edaphon.Investigated soil samples were taken from polluted andnon-polluted places in the Baix Llobregat near Barcelona (Catalonia, NE Spain). Soil samples were taken from the top ofsoil (0–5 cm) without a litter. Soil analysis were done, determining percentage shares of coarsefragments, coarse sand, fine sand, coarse silt, fine silt, clay,CaCO₃, organic matter as well as water pH and conductivityCE (1:5 [mS cm^-1]). Also were determined (in mg kg^-1)quantities of heavy metals, as Fe, Al, Mn, Zn, Cr, Ni, V, Cu, Cd, Pb.The soil respiration was investigated in temperatures15 and 30 °C and with controlled humidity.The respiration in 30 °C is number of times greater thenin 15 °C both for polluted and non-polluted soils.Particularly high coefficients of correlation between the soilrespiration and soil pollution in polluted soils were obtainedfor Pb: r = 0.75 in 15 °C and r = 0.98 in30 °C; for Ba: 0.90 and 0.57; for V: 0.99 and 0.81. In non-polluted soils highest correlation coefficients are for Pb: r = 0.70 in 15 °C; Fe: 0.60 and 0.72; Al: 0.68 and0.64; Mn: 0.51 and 0.66; Ba: 0.63 and 0.61; Cr: 0.94 and0.70; Ni: 0.64 and 0.65; Cu: 0.69 and 0.48; as well as V: 0.62in 15 °C; and Cd: 0.69 in 15 °C.This way the soil respiration could be a good indicator of the soil pollution.     

Characterization of microbial communities from coastal waters using microarrays

Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish.     

A Metamodeling Approach for Integrated Assessment of Air Quality Policies

A method is proposed to build integrated models (also called Metamodels) aimed at quantifying the economic efficiency of air quality policies. This Metamodeling approach is based on the coupling of two complementary models, that operate at different scales in space and time, and which represent the economic and the physical and chemical processes, respectively. The joint consideration of the physico-chemical and techno-economic structure of the pollution control problems permits a comprehensive evaluation of air pollution abatement strategies. The motivating pollution control problems include urban-regional air quality management through efficient energy and traffic control policies. A pilot study, exploiting data collected in the Geneva canton (Switzerland), is used to demonstrate the potential of the approach.     

几种土壤质量评价方法的比较

分别用Ｔ值分级法，综合指数法，模糊数学综合评判法，灰色聚类法，等斜率灰色聚类法，宽域灰色聚类法对湖南某地１０个监测点的土壤质量现状进行评价，通过比较，认为宽域灰色聚类法较好。