真鲷去毒相关基因cDNA序列的克隆与肝脏组成型表达分析

赤潮发生时产生的一些海洋生物毒素对人类和海洋动物的安全形成潜在的威胁甚至导致死亡.为从分子水平探讨鱼类中海洋藻毒素的去毒分子机理,采用RT-PCR法克隆了真鲷(Pagrus major)肝脏芳香烃受体核转位蛋白(ARNT)和I时相异生素代谢酶细胞色素P4501A(CYP1A)基因cDNA核心序列,同时,应用半定量RT-PCR方法,以β-肌动蛋白作为外参照,在其指数期增长的范围内研究了芳香烃受体(AHR)、ARNT、CYP1A、II时相异生素代谢酶alpha型谷胱甘肽S-转移酶(GSTA1、GSTA2)、rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70(HSP70)基因组成型表达水平.结果发现,真鲷ARNT、CYP1A基因cDNA核心序列片段分别长438bp和908bp,分别编码146和302个氨基酸.序列同源性分析发现,真鲷与门齿鲷(Stenotomus chrysops)、石首鱼(Micropogon undulatus)ARNT基因氨基酸序列同源性高达97.2%、95.2%,与斑马鱼(Brachydanio rerio)、人、大鼠、小鼠ARNT同源性较低(77.2%～79.3%).真鲷与门齿鲷、金头鲷(Sparus auratus)、欧洲川鲽(Rhombus maximus)、欧洲海鲈(Dicentrachus labrax)CYP1A基因氨基酸序列同源性较高,为84.8%～94.0%,与斑马鱼、人、小鼠CYP1A同源性较低,为59.6%～77.8%.真鲷肝脏AHR、ARNT、CYP1A、GSTA1、GSTA2、GSTR和HSP70基因组成型表达水平分别为(25.32±6.56)%、(26.22±4.24)%、(146.5±16.06)%、(55.42±3.75)%、(48.82±10.89)%、(79.47±3.13)%、(107.42±14.34)%.     

船载雷达海冰监测及预报技术在辽东湾冰区油轮外输作业中的应用

渤海的辽东湾是世界上典型的海湾结冰区,位于冰区中的石油平台油轮外输作业中,流冰连续不断地漂移、聚集形成堆积冰,给海上油轮的外输作业安全带来严重危害。本文概述了在国内首次利用船载雷达,在该湾冰区石油平台上,现场采用雷达海冰监测预报技术系统,进行遥感海冰数据源的获取,图像的解译和数值化处理,得到流冰类型、冰厚度、流冰密集度、流冰速度、方向、流冰漂移轨迹等要素。同时首次现场实时计算出雷达冰漂流场矢量图,并开展了雷达海冰数值跟踪预报,在冰区油轮外输作业中得到很好应用,为雷达技术对水体和海冰监测和预报、遥感应用新领域提供科学依据。     

基于山江海视角与SRP模型的桂西南-北部湾生态环境脆弱性评价

为揭示桂西南喀斯特-北部湾海岸带生态环境脆弱性,本研究基于山江海视角,采用生态敏感度-生态恢复力-生态压力度(SRP)模型、无纲量化模型、层次分析法,结合生态环境脆弱性指数,对该区域进行了生态环境脆弱性的评价,为该区域的生态环境治理和恢复提供科学的理论基础和技术指导.结果表明:(1)2000～2018年三期生态环境脆弱...     

Standardizing the Methodology of Sperm Cell Test with Paracentrotus Lividus

The sperm cell toxicity test with Paracentrotus lividus is widely used in Italy to assess the quality of complex aqueous matrices, but at present there is a shortage of standardized methodologies for the bioassay performance. In this work several critical points were considered, in order to verify the reliability of this bioassay and to improve its standardization.In particular, we have studied the differences in EC50 values at different sperm: egg ratios and with different quantities of gametes; the influence of sperm and eggs on fertilization rate and the influence of different recipients materials (plastic or glass). At the same time, it was evaluated if the obtained EC50 values were in accordance with those reported in literature.Experiments performed yielded EC50 values for copper ranging from 16.54 μ g/l to 69.59 μ g/l, with a total mean value ± SD of 39.79 μ g/l ± 11.17. These data fit with those found in literature for other echinoid species. According to the same authors, poor fertilization was probably due to the effects on sperm and not on eggs, and moreover the linear regression analysis performed on each experiment suggested that the best amount of eggs to use in the test was 1000.Results from the comparison between the performance of the test in plastic or glass tubes showed an higher success in fertilization rate using borosilicate glass tubes.     

Evaluation of the Fertilization Capability of Paracentrotus Lividus Sea Urchin Storaged Gametes by the Exposure to Different Aqueous Matrices

Several experiments were performed to evaluate the fertilization capability of Paracentrotus lividus sea urchin gametes, which are usually used in fertilization tests, but which, until now, were employed only for few hours after spawning.The aim of this work is to evaluate the possibility of performing the bioassay for longer periods, employing ‘conserved' gametes. As far as the method, the sperm was stored ‘dry' at 4 ^∘C; eggs were conserved in an antibiotic solution at 4 ^∘C, and they were exposed both to copper solutions as reference toxicant and to aqueous matrices.The use of conserved gametes in the fertilization bioassay lead to EC50 values which were acceptable for about 3 days. Moreover, these experiments permitted to identify the limiting factor: the male gamete.     

两栖装备入舰引导系统方案研究

目的针对两栖装备上下登陆舰时自动化程度低、无法全天候实施的问题,提高恶劣海况和黑夜条件下的登舰作业能力,提出一种两栖装备入舰引导系统设计方案。方法依托装备再制造升级技术手段,通过水下探测器接收登陆舰发射的超声波定位脉冲信号,获得装备相对位置和路线规划信息,有效规避航线水下障碍物,提高装备登舰的准确度和效率。结果针对系统发出的超声波信号部分被水下超声接收器接收的情况,对海水、海洋环境噪声和海洋混响等因素对声波信号传输特性的影响展开研究,重点分析了浅海海域的季节性声速剖面、传播过程中的声强损失,对海洋环境噪声、海底和海面的等效平面波混响级进行数学建模,通过静态和动态试验,验证了模型推导的准确性和数据的可靠性。结论通过浅海多径传播信号合成模型和双路径传播的信号衰减模式的推导,为求解声波传播模型的波动方程奠定了基础。     

Persistent organic pollutants in sea bass (Dicentrarchus labrax L.) in two fish farms in the Mediterranean Sea

We analyzed polychlorobiphenyls (PCBs), perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) in the edible part of farmed sea bass reared in two fish farms in Liguria (Northern Italy). The aim was to determine the contamination levels and human exposure to these chemicals through fish consumption. Concentrations of “dioxin-like” PCBs (DL-PCBs) ranged from 0.033 to 0.759 pg Σ_TEQ-PCB g⁻¹ whole weight (w.w.) in fish farm 1 and from 0.032 to 1.60 pg Σ_TEQ-PCB g⁻¹ w.w. in fish farm 2, and the six indicators of “non-dioxin-like” (NDL-PCBs) from 0.538 to 9.33 ng Σ_6PCB g⁻¹ w.w. and from 1.62 to 27.6 ng Σ_6PCB g⁻¹ w.w. Concentrations were generally lower in farm 1 than in farm 2. One reason for this difference might be the proximity of farm 2 to the seaport of La Spezia, which could be a punctual source of pollutants influencing the contamination of the water in the farm. Principal component analysis (PCA) showed differences also in the congeners profiles for the two sites, with higher-chlorinated PCBs more abundant in farm 1, and lower-chlorinated PCBs were more abundant in farm 2. Most of the concentrations of PFOS and PFOA were below the limit of detection (LOD 0.05 ng g⁻¹ w.w.). Only about 10% of the samples analyzed had levels slightly higher than the LOD. Assessments of exposure using these data showed that consumption of farmed fish may contribute significantly to PCBs through the diet, whereas the contribution of PFOS and PFOA seems to be low.     

基于EOS/MODIS卫星遥感数据的渤海海冰变化趋势及特征应用研究

根据卫星遥感数据的分辨率、采集频次、获取方式等特点,将MODIS卫星遥感数据应用到渤海海冰遥感监测中。通过海冰在可见光和红外光波段的光谱存在特定差异的特性,对海冰发生面积进行提取。同时对渤海海冰进行了遥感监测,并完整地监测到海冰从发生到极盛再到消退的全过程。同时,在海冰发生最为严重的时段,对辽东湾海域进行了密集监测。研究结果表明,MODIS卫星遥感数据在海冰监测中有着及时、准确的优势。     

石墨炉原子吸收法测定海水中Pb——以0.1NHNO3抗坏血酸-NaI作基体改进剂

研究了以0.1N HNO3抗坏血酸-NaI作基体改进剂测定海水中Pb的方法,讨论并确定了测定的最佳条件.结果表明在仪器最佳条件下,以0.1N HNO3抗坏血酸-NaI作基体改进剂可有效消除海水基体干扰,检出限为1.2μg/L,实际样品相对标准偏差小于4%,加标回收率为95%～104%.     

Seasonal variation of organochlorine contaminants in bonito (Sarda sarda L. 1758) and anchovy (Engraulis encrasicolus L. 1758) in Black Sea region, Turkey

The concentrations of indicator polychlorinated biphenyls (PCBs) and organochlorine insecticides were determined in bonito (Sardasarda L. 1758) and anchovy (Engraulisencrasicolus L. 1758) from the Black Sea, Turkey. Concentrations of total indicator PCBs ranged between <1-17.0 in bonito, and <1-17.5 ng/g fresh weight in anchovy, and total of 1,1,1-trichloro-2,2-bis-chlorophenyl-ethane and its metabolites’ (DDTs) concentrations ranged between 13.4-26.3, and 2.96-19.0 ng/g fresh weight in bonito and anchovy respectively. PCB 52, p,p′-DDE and endosulfan (α + β) were found dominant in both of the fish species. Except endosulfan, and some DDT metabolites, none of the studied organochlorine pesticides was detected in the fish samples. Concentrations of PCBs in anchovy were found higher than those in bonito, whereas DDT and endosulfan concentrations were found similar in both of the fish species. All of the fish samples had residue concentrations below the maximum residue limits (MRL) recommended by FAO/WHO Codex Alimentarius Commission.