C/N比对嗜酸细菌X-29产氢能力及其酶活性的影响

C/N比影响细菌的物质和能量代谢,为了提高产氢细菌的产氢效能,通过间歇产氢实验和酶活性分析方法,分析了在不同C/N比下嗜酸产氢细菌X-29的产氢能力以及氢化酶和乙醇脱氢酶的活性表达情况.研究结果表明,C/N比对产氢细菌的代谢及其相关酶的表达有显著的影响.虽然在不同C/N比下单位生物量的液相末端发酵产物差异不大,但是产氢能力存在显著的差异,当C/N比为14时产氢细菌X-29具有最大累积产氢量2 210.9mL/g.在不同C/N比下氢化酶的表达活性不同,氢化酶活性随着发酵的进行达到高峰后迅速降低,氢化酶的表达周期较短.乙醇脱氢酶活性随着代谢进程逐渐升高后而趋于平稳,不同C/N比时表达活性差异较小,表达周期较长.在C/N比为14时,氢化酶和乙醇脱氢酶的活性最高,分别为2.8μmol·(min·mg)^-1和33.2μmol·(min·mg)^-1.     

Competition gives way to cooperation: rethinking Sino-Indian relations in climate change negotiations

In global climate change politics, China and India have worked closely with each other, representing two big emitters from the developing world. This article reviews Sino-Indian cooperation during four UN climate change negotiations from 2009 to 2012. Their collaboration echoed the perception of neorealism in international relations (IR) studies that states will seek “external balancing” for maximizing national “relative gains”. China and India are expected to continue their cooperation on climate change issues when sharing the same structural position as big economies and big emitters, the same pressure in carbon emission reductions, and the same goal of improving living standards for their citizens. Encountering the devastating impacts of climate change and the deadlock in responsibility division in current global climate change governance, this work seeks to shed light on the evolving multilateral governance environment and its complex implications for Sino-Indian relations from an IR perspective.     

在役压力容器的动态检测技术与研究进展

根据笔者多年声发射检测与评价技术的研究成果并综合分析不同发展阶段的有关文献，论述了压力容器结构完整性检测与综合评估的意义，分析其合理性和难度，并对这种动态检测技术在评价压力容器结构完整性方面的研究进展作了较全面的评述     

安全是一切生产活动的基本前提

从抓安全生产的必要性和迫切性入手,着重分析了安全生产的实践中存在的诸多问题,提出了有针对性的解决问题的建议,对安全生产工作有一定的借鉴意义。     

Biodegradation of Methoxychlor and Its Metabolites by the White Rot Fungus Stereum hirsutum Related to the Inactivation of Estrogenic Activity

The white rot fungus Stereum hirsutum was used to degrade methoxychlor [2,2,2-trichloro-1,1-bis(4-methoxyphenyl)ethane] in culture and the degraded products were extensively determined. The estrogenic activity of the degraded products of methoxychlor was examined using cell proliferation and pS2 gene expression assays in MCF-7 cells. S. hirsutum showed high resistance to methoxychlor 100 ppm, and the mycelial growth was fully completed within 8 days of incubation at 30°C. Methoxychlor in liquid culture medium was gradually converted into 2,2-dichloro-1,1-bis(4-methoxyphenyl)ethane, 2,2-dichloro-1,1-bis(4-methoxyphenyl)ethylene, 2-chloro-1,1-bis(4-methoxyphenyl) ethane, 2-chloro-1,1-bis(4-methoxyphenyl) ethylene, and 1,1-bis(4-methoxyphenyl)ethylene, indicating that methoxychlor is dominantly degraded by dechlorination and dehydrogenation. MCF-7 cells were demonstrated to proliferate actively at the 10^?5 M concentration of methoxychlor. However, cell proliferation was significantly inhibited by the incubation with methoxychlor culture media containing S. hirsutum. In addition, the expression level of pS2 mRNA was increased at the concentration (10^?5 M) of methoxychlor. The reductive effect of S. hirsutum for methoxychlor was clear but not significant as in the proliferation assay.     

日地环境对人类自发攻击性事件的影响

考察了日地空间物理环境扰动与人类自发攻击性群体性事件发生率之间相关性。统计分析发现，两者之间的相关程度很高，历元叠加后相关系数可达０．８４和０．７９、这表明日地空间物理环境对人类攻击性本能和情绪具有显著的调节作用，导致全球自发攻击性群体事件年发生率具有１１ａ准周期波动特征。其作用机制可能是地磁场活动和大气温度的影响。     

黑龙滩水库水体生态系统酶活力及其生态学意义研究

本文黑龙滩水库水体生态系统中脲酶与磷酸酶活力及其与多种水质参数之间的关系进行了研究，结果表明：（１）酶活力与水质，水温变化呈出现一致的动态变化规律。（２）黑龙滩水库水体中脲酶来源与水生生物关系不大，而磷酸酶主要来自浮游植物和水生细菌。（３）水体中脲酶活力受水中总氮浓度瓣影响，而磷酸酶活力不受水中磷酸盐的抑制。     

Screening of wood rotting fungi potentially useful for the degradation of organic pollutants

Many papers have shown that white rot fungi can degrade aromatic pollutants under laboratory conditions, but few report field scale trials. Here we report the first steps in the development of a remediation system for Greek conditions. A review of the available organochlorine compound pollution information in Greece is presented. White rot fungi isolated from sites in Greece have been screened for growth rate and ligninolytic activity, using decolourisation of the dye Poly R-478 as an indicator of enzyme activity. Use of white rot fungi under field conditions in Greece will require bioaugmentation to be effective at high temperatures and low water activity for much of the year. The most potent strains have been selected under a range of conditions and have been challenged with priority pollutants to determine their degradative ability under laboratory conditions and subsequently ex situ in soil.     

Bioremediation of Soil Degraded by Sewage Sludge: Effects on Soil Properties and Erosion Losses

Soils in the Mediterranean area are very prone to erosion due to the loss of organic matter and the consequent lack of protective vegetation. In this experiment a Mediterranean degraded soil with a 15% slope was amended at a rate of 250 t ha^–1 wet weight with sewage sludge and with a mixture of sewage sludge and barley straw (70% carbon from sewage sludge and 30% from the straw) in order to study their influence on soil structure recovery and hence the soilss resistance to erosion processes. Both types of organic amendment led to an improvement in several soil properties (physical, biological, and microbiological) as a result of the spontaneous growth plant covering that became evident three months after amendment. This vegetation remained throughout the two years of the experiment and prevented the water erosion processes that normally precede soil degradation. Amendment by sewage sludge alone reduced soil loss by 80% compared with the control soil, while the mixture that included both sewage sludge and barley straw reduced losses by 84%, both reducing runoff by 57%. The amended soils showed increases in the percentage of stable aggregates, the levels of the total and water-soluble C fractions, microbial biomass C, basal respiration, and the activity of the different enzymes involved in the biogeochemical cycles of C, N, and P. The results confirm the usefulness of sewage sludge as an organic amendment for recovering damaged soils.