三七渣固态发酵生产绿色木霉的工艺条件研究

考察了发酵时间、孢子液接种量、固态发酵培养基装料量、培养温度、光照条件等对三七渣固态发酵生产绿色木霉的影响,并采用正交实验优化了发酵工艺条件。结果表明,发酵过程受工艺条件的影响较大,孢子液接种量、固态发酵培养基装料量、发酵时间3个因素的交互作用极显著(p0.01),这3个因素以及它们之间的交互作用对实验结果的影响程度排序为:交互作用固态发酵培养基装料量孢子液接种量发酵时间;在最佳的发酵工艺条件(即培养温度24℃、无光照、接种量20%(1g三七渣接种2mL绿色木霉孢子液)、固态发酵培养基装料量12.5g、发酵时间8d)下,平均产孢量可达10.10×109 cfu/g。     

虫草头孢菌发酵废液成分分析及其再利用

利用高效液相色谱法、氨基酸自动分析法和分光光谱法对虫草头抱菌发酵液成分进行了测定和分析,发现内含多种营养成分,完全有可能再次利用．用由该发酵液经加热、加活性炭和中药添加剂处理制备而成的组合培养液来培养哈茨木霉,具有产孢量高、培养成本低的优点．试验还发现,以组合培养液和麦麸为主要成分的新型固体培养基完全可以代替纯麦麸培养基．     

Chemical synthesis of 6-pentyl-2H-pyran-2-one: a natural antifungal biosynthesized by Trichoderma spp.

Trichoderma spp. biosynthesize 6-pentyl-2H-pyran-2-one (6-PP), a natural antifungal pyrone which could be used as biological control agent (BCA). Unfortunately, biotechnical processes are limited by inhibition of biomass at high concentration of 6-PP. We report herein a new easy synthesis of this natural pyrone, using readily available starting materials. This synthesis, compatible with a large production scale, permit to obtain overweight amounts of 6-PP that in biotechnological routes.     

添加剂对绿色木霉JQF-04纤维素酶热稳定性的影响

如何提高酶蛋白的热稳定性是分子生物学、微生物学、生化工程学等学科长期所关注的重要研究课题之一.本文研究了多种添加剂对绿色木霉纤维素酶热稳定性的影响.在60℃的溶液中,多元醇(乙二醇、甘油、赤藓糖醇、木糖醇和山梨糖醇)能提高该酶的热稳定性,随着浓度的增加,赤藓糖醇、木糖醇和山梨糖醇促进酶的热稳定性呈线性增高;适当的多元醇分子长度对该酶的热稳定性有最优的保护效应;不同浓度和不同分子量的聚乙二醇对该酶的热稳定性具有明显的影响;在无机盐中,单价金属阳离子比二价金属阳离子更能显著地提高该酶的热稳定性;酶液溶剂的改变直接影响着该酶的热稳定性,该酶在D2O溶液中比在水溶液中稳定,其酶活半衰期延长了2.6倍.研究表明,热环境使酶蛋白分子的螺旋结构发生变化而失活,但某些溶质和溶剂的存在可能通过作用于蛋白质分子的三维结构而影响该酶的热稳定性.图7参15     

Tolerance and biosorption of copper （Cu） and lead （Pb） by filamentous fungi isolated from a freshwater ecosystem

Filamentous fungi are able to accumulate significant amount of metals from their environment. The potential of fungal biomass as agents for biosorption of heavy metals from contaminated sediments is currently receiving attention. In the present study, a total of 41 isolates of filamentous fungi obtained from the sediment of the Langat River, Selangor, Malaysia were screened for their tolerance and uptake capability of copper (Cu) and lead (Pb). The isolates were identified as Aspergillus niger, A. fumigatus, Trichoderma asperellum, Penicillium simplicissimum and P. janthinellum. A. niger and P. simplicissimum, were able to survive at 1000 mg/L of Cu(II) concentration on Potato Dextrose Agar (PDA) while for Pb, only A. niger survived at 5000 mg/L concentration. The results showed that A. niger, P. simplicissimum and T. asperellum have a better uptake capacity for Pb compared to Cu and the findings indicated promising biosorption of Cu and Pb by these filamentous fungi from aqueous solution. The present study was also determined the maximum removal of Cu(II) and Pb(II) that was performed by A. niger. The metal removal which occurred at Cu(II) 200 mg/L was (20.910 0.581) mg/g and at 250 mg/L of Pb(II) was (54.046 0.328) mg/g.