春蚕期桑叶氟化物含量变化规律分析与研究

春蚕桑叶氟化物中毒引起政府部门的高度重视。通过对历年来桑叶含氟量监测数据统计分析，找出春蚕期桑叶含氟量日均值变化规律，并对规律成因进行探讨研究，为春蚕防氟工作提供科学依据。     

Sensitivity analyses of woody species exposed to air pollution based on ecophysiological measurements

GOAL, SCOPE AND BACKGROUND: Air pollution has been of a major problem in the Pearl River Delta of south China, particularly during the last two decades. Emissions of air pollutants from industries have already led to damages in natural communities and environments in a wide range of the Delta area. Leaf parameters such as chlorophyll fluorescence, leaf area (LA), dry weight (DW) and leaf mass per area (LMA) had once been used as specific indexes of environmental stress. This study aims to determine in situ if the daily variation of chlorophyll fluorescence and other ecophysiological parameters in five seedlings of three woody species, Ilex rotunda, Ficus microcarpa and Machilus chinensis, could be used alone or in combination with other measurements for sensitivity indexes to make diagnoses under air pollution stress and, hence, to choose the correct tree species for urban afforestation in the Delta area. METHODS: Five seedlings of each species were transplanted in pot containers after their acclimation under shadowing conditions. Chlorophyll fluorescence measurements were made in situ by a portable fluorometer (OS-30, Opti-sciences, U.S.A). Ten random samples of leaves were picked from each species for LA measurements by area-meter (CI-203, CID, Inc., U.S.A). DW was determined after the leaf samples were dried to a constant weight at 65 degrees C. LMA was calculated as the ratio of DW/LA. Leaf N content was analyzed according to the Kjeldhal method, and the extraction of pigments was carried out according Lin et al. RESULTS AND DISCUSSION: The daily mean Fv/Fm (Fv is the variable fluorescence and Fm is the maximum fluorescence) analysis showed that Ilex rotunda and Ficus microcarpa were more highly resistant to pollution stress, followed by Machilus chinensis, implying that the efficiency of photosystem II in I. rotunda was less affected by air pollutants than the other two species. Little difference in daily change of Fv/Fm in I. rotunda between the polluted and the clean site was also observed. However, a relatively large variation of Fv/Fm appeared in the other two species, particularly in M. chinensis, suggesting that they were more sensitive to air pollutants than I. rotunda. The mean LA was reduced for all species growing at the polluted site. The mean LMA for all species exceeded the sclerophylly threshold given by Cowling and Campbell and increased for those under pollution stress, which could be explained as one of the acclimation strategies for plants to air pollution stress. Little difference in leaf chlorophyll content was observed in F. microcarpa and M. chinensis, while remarkable differences were found in I. rotunda growing at the polluted and the clean site. Content of leaf carotenoids was largely reduced in I. rotunda growing at the polluted site, but increased in F. microcarpa and M. chinensis, compared with plants growing at the clean site. Plants growing at the clean site had a lower leaf N content than those growing at the polluted site. In addition, species with a higher resistance to pollution stress showed less difference in leaf N content than those sensitive species. CONCLUSION: Based on Fv/Fm measurements of the three woody species, I. rotunda showed the highest resistance to air pollutants from ceramic industries, followed by F. microcarpa. M. chinensis was the most sensitive species to air pollution, had lowest capacities to cope with the air pollution stress, which was consistent with visual injury symptoms observed in the crown profiles of plants at the polluted site. Fv/Fm, LAM, LA, leaf pigments and N content could be used alone or in combination to diagnose the extent of the physiological injury. The ratio of Fv/Fm, however, was the best and most effective parameter. RECOMMENDATION AND OUTLOOK: Tree species which have higher air-pollutant resistance, as diagnosed by such ecophysiological parameters, should be considered first and planted widely for urban afforestation or forest regeneration in areas where the forest was seriously degraded or forest health was markedly effected by the same kind of air pollutants.     

交通污染区城市绿化植物硫含量分布

为探讨城市绿化植物对交通环境中SO2的响应，以远离城市道路的相对清洁区公园生长的植物为对照植物，采集了广东省被广泛栽种的18种园林绿化植物的不同方位的叶片和树皮样品，用硫酸钡比浊法测定了每种植物样品在交通污染区面向机动车行驶方向、面向人行道方向及相对清洁区的植物样品等不同方位的植物叶片和树皮硫含量，分析了绿化植物在同一株植物的不同生长部位和不同环境中硫含量的分布特征。结果表明，交通污染区所有样品的叶片硫和树皮硫含量均高于相对清洁区的样品。其中，远离工厂的交通污染区生长的同一株植物，离机动车道越近，硫含量越高；靠近工厂的交通污染区的同一植物，离工厂近的部位的植物树皮硫含量高于面向机动车道一侧的植物硫含量。     

Oviposition by butterflies on young leaves: Investigation of leaf volatiles

Summary Various butterflies select young foliage on which to lay their eggs; volatiles emitted by young and old leaves have been compared (by sorption enrichment, followed by GC-MS) to gauge possible qualitative and quantitative differences between the two age groups. The plants investigated are cabbage (Brassica oleracea), two milkweeds (Asclepias syriaca andA. curassavica), the bitter orange (Citrus aurantium) and the lime (C. aurantiifolia). The chemical compounds identified belong to three classes, isoprenoids, fatty acid derivatives and benzenoids. Quantitative differences were found between young and old leaves, of which a few may be characteristic of young leaves only. Thirty-four single trials withDanaus plexippus exposed to volatiles from young and old leaves are recorded.     

Induced alkaloid defence inAtropa acuminata in response to mechanical and herbivore leaf damage

Summary Measurement of tropane alkaloid content in leaves ofAtropa acuminata after mechanical damage showed a maximum increase to 153% of the control 8 days later. There were no changes in the root or stem after similar damage. The plant responded to repeated mechanical damage by doubling its alkaloid content at 11 days after the initial wounding. But on further treatments, there was a slight decrease in alkaloid content with time. Mollusc feeding produced an increase of 164% in alkaloid content after 4 days. These results indicate that induced defence systems in angiosperms can vary considerably from plant species to plant species and that the effects of mechanical damage may differ in different parts of the same plant.     

Uptake and metabolism of [<Superscript>14</Superscript>C]rinderine and [<Superscript>14</Superscript>C]retronecine in leaf-beetles of the genus <Emphasis Type="Italic"> Platyphora</Emphasis> and alkaloid accumulation in the exocrine defensive secretions

Summary. Sequestration and processing of pyrrolizidine alkaloids (PAs) by leaf beetles of the genus Platyphora were investigated. Tracer experiments with labeled alkaloids were performed with P. eucosma feeding on Koanophyllon panamense (Asteraceae, tribe Eupatorieae). P. eucosma catalyzes the same reactions previously demonstrated for P. boucardi specialized to Prestonia portobellensis (Apocynaceae): (i) epimerization of rinderine to intermedine; (ii) esterification of retronecine yielding insect-specific PAs; (iii) efficient transport of the PAs as free bases into the defensive secretions. P. bella feeding on Tournefortia cuspidata (Boraginaceae) shows the same sequestration behavior and ability to synthesize the specific retronecine esters. P. ligata, a species phylogenetically closely related to the PA adapted species and clustering in the same clade, but feeding on a host plant devoid of PAs, feeds easily on PA treated host-plant leaves, but does not sequester or metabolize PAs. P. kollari a species clustering outside the PA clade refused to feed on its food-plant leaves painted with PAs. The results are discussed in relation to host-plant selection of the PA adapted species and the role of PAs in chemical defense. Received 20 September 2002; accepted 18 November 2002.     

宁南霉素诱导烟草酸性病程相关蛋白的研究

研究了宁南霉素对烟草酸性病程相关蛋白的诱导表达．结果表明，宁南霉素可系统性地诱导烟草叶片中酸性病程相关蛋白的高效表达．与水杨酸相比，宁南霉素诱导酸性病程相关蛋白的表达水平较高，持续时问较长．此外，在同一品种不同形态叶片烟草植株中，宁南霉素对植物酸性病程相关蛋白的诱导存在差异．图6表3参20     

钢板弹簧断裂失效行为研究

目的研究汽车钢板弹簧断裂失效的原因。方法通过化学成分分析、硬度测试、断口形貌分析、金相组织分析等手段研究汽车钢板弹簧的失效行为。结果由于表面脱碳层过深,使得表面疲劳强度及耐蚀性降低,同时因长时间腐蚀作用在表面产生腐蚀坑及腐蚀裂纹,并且在循环外力作用下,腐蚀缺陷处产生应力集中,早期裂纹萌生于表面腐蚀缺陷处并扩展,最终导致腐蚀疲劳断裂。结论通过控制热处理条件、增加加工余量并去除脱碳层、表面喷丸处理等措施,钢板弹簧表面耐蚀及抗疲劳性能大幅提高,可有效避免脱碳引起的腐蚀疲劳断裂。     

The analysis of influence of individual and environmental factors on 2-wheeled users’ injuries

Objective: Powered 2-wheeled motor vehicles (PTWs) are one of the most vulnerable categories of road users. Bearing that fact in mind, we have researched the effects of individual and environmental factors on the severity and type of injuries of PTW users. The aim was to recognize the circumstances that cause these accidents and take some preventive actions that would improve the level of road safety for PTWs.

Methods: In the period from 2001 to 2010, an analysis of 139 road accidents involving PTWs was made by the Faculty of Transport and Traffic Engineering in Belgrade. The effects of both individual (age, gender, etc.) and environmental factors (place of an accident, time of day, etc.) on the cause of accidents and severity and type of injuries of PTWs are reported in this article. Analyses of these effects were conducted using logistic regression, chi-square tests, and Pearson's correlation.

Results: Factors such as categories of road users, pavement conditions, place of accident, age, and time of day have a statistically significant effect on PTW injuries, whereas other factors (gender, road type; that is, straight or curvy) do not. The article also defines the interdependence of the occurrence of particular injuries at certain speeds. The results show that if PTW users died of a head injury, these were usually concurrent with chest injuries, injuries to internal organs, and limb injuries.

Conclusions: It has been shown that there is a high degree of influence of individual factors on the occurrence of accidents involving 2-wheelers (PTWs/bicycles) but with no statistically significant relation. Establishing the existence of such conditionalities enables identifying and defining factors that have an impact on the occurrence of traffic accidents involving bicyclists or PTWs. Such a link between individual factors and the occurrence of accidents makes it possible for system managers to take appropriate actions aimed at certain categories of 2-wheelers in order to reduce casualties in a particular area. The analysis showed that most of the road factors do not have a statistically significant effect on either category of 2-wheeler. Namely, the logistic regression analysis showed that there is a statistically significant effect of the place of accident on the occurrence of accidents involving bicyclists.     

植物法改性类Fenton反应催化剂降解甲基橙

采用银杏叶和桑叶提取液制备了改性类Fenton反应催化剂并进行了表征分析,研究了溶液初始p H、反应温度、催化剂加入量、甲基橙初始质量浓度等因素对甲基橙降解率的影响,同时考察了催化剂的重复使用效果。表征结果表明:制备出的催化剂为Fe_2O_3和Fe OOH的混合物;桑叶改性催化剂的粒径分布较银杏叶改性催化剂均匀,粒径较小,比表面积较大。实验结果表明:在初始p H为6.23、反应温度60℃、催化剂用量1 g/L、甲基橙初始质量浓度100 mg/L的条件下,银杏叶改性催化剂的甲基橙降解率为99.40%,桑叶改性催化剂的甲基橙降解率为99.96%;碱性条件下,甲基橙降解率仍接近100%,扩宽了反应的p H适用范围,为碱性条件下处理偶氮染料提供了新思路;催化剂重复使用6次之后,甲基橙降解率仍可达到99%。根据反应前后溶液的紫外-可见吸收光谱,初步探讨了降解机理。