BOD生物传感器的研制与应用

介绍了ＢＯＤ生物传感器的原理、研制过程以及实际测定。该仪器测定ＢＯＤ的周期为３０ｍｉｎ，有较高的精密度和准确度，与稀释法有良好的相关性，可以及时信息反馈，特别适用于污水处理过程中的控制分析以及ＢＯＤ的快速测定。     

BOD微生物传感检测仪中高效微生物膜的研究

为了制备高效微生物膜,从污水中筛选菌种（QT2061）,经BIOLOG鉴定为枯草芽孢杆菌。并以聚乙烯醇为包埋剂制作BOD生物传感器微生物膜,通过对国家标准样品及几种典型水样的检测,结果表明,该微生物传感器的响应时间为8 min,可连续稳定测试10 d以上;对国家标准溶液的测试结果准确度较高,相对误差≤5%;对实验中几种水样均有响应,测试结果与5日法的测试结果有良好的相关性（相对偏差≤5%）。     

微生物传感器测定水中BOD的研究进展

详细介绍了BOD微生物传感器的构造、基本工作原理以及微生物膜的制备和固定化技术;分析了近年来BOD微生物传感器的发展情况及其使用过程中的存在问题。根据BOD传感器的研究现状提出今后的研究方向和工作重点。     

Mercaptobenzothiazole-on-gold organic phase biosensor systems: 1. Enhanced organosphosphate pesticide determination

This paper reports the construction of the gold/mercaptobenzothiazole/polyaniline/acetylcholinesterase/polyvinylacetate (Au/ MBT/PANI/AChE/PVAc) thick-film biosensor for the determination of certain organophosphate pesticide solutions in selected aqueous organic solvent solutions. The Au/MBT/PANI/AChE/PVAc electrocatalytic biosensor device was constructed by encapsulating acetylcholinesterase (AChE) enzyme in the PANI polymer composite, followed by the coating of poly(vinyl acetate) (PVAc) on top to secure the biosensor film from disintegration in the organic solvents evaluated. The electroactive substrate called acetylthiocholine (ATCh) was employed to provide the movement of electrons in the amperometric biosensor. The voltammetric results have shown that the current shifts more anodically as the Au/MBT/PANI/AChE/PVAc biosensor responded to successive acetylthiocholine (ATCh) substrate addition under anaerobic conditions in 0.1 M phosphate buffer, KCl (pH 7.2) solution and aqueous organic solvent solutions. For the Au/MBT/PANI/AChE/PVAc biosensor, various performance and stability parameters were evaluated. These factors include the optimal enzyme loading, effect of pH, long-term stability of the biosensor, temperature stability of the biosensor, the effect of polar organic solvents, and the effect of non-polar organic solvents on the amperometric behavior of the biosensor. The biosensor was then applied to detect a series of 5 organophosphorous pesticides in aqueous organic solvents and the pesticides studied were parathion-methyl, malathion and chlorpyrifos. The results obtained have shown that the detection limit values for the individual pesticides were 1.332 nM (parathion-methyl), 0.189 nM (malathion), 0.018 nM (chlorpyrifos).     

Comparison of naphthalene bioavailability determined by whole-cell biosensing and availability determined by extraction with Tenax

A rapid biological method for the determination of the bioavailability of naphthalene was developed and its value as an alternative to extraction-based chemical approaches demonstrated. Genetically engineered whole-cell biosensors are used to determine bioavailable naphthalene and their responses compared with results from Tenax extraction and chemical analysis. Results show a 1:1 correlation between biosensor results and chemical analyses for naphthalene-contaminated model materials and sediments, but the biosensor assay is much faster. This work demonstrates that biosensor technology can perform as well as standard chemical methods, though with some advantages including the inherent biological relevance of the response, rapid response time, and potential for field deployment. A survey of results from this work and the literature shows that bioavailability under non-equilibrium conditions nonetheless correlates well with K_oc or K_d. A rationale is provided wherein chemical resistance is speculated to be operative.     

生物传感器应用于环境监测的新进展

综述生物传感器应用于环境监测的最新进展 ,包括对砷化物、硫化物和杀虫剂及除草剂残留物的检测 ,对废水水质的在线监测 ,对BOD和氨氮的测定 ,对酚类和有气味的化合物的检测以及对大气和废气的监测等方面。     

生物传感器BOD快速测定仪的研究进展

生化需氧量(biochemical oxygen demand,BOD)作为表征水体中种类繁多的有机污染物的集合参数,在水质评价、污染监测以及废水处理厂的运行分析等方面发挥着重要作用.然而,传统的BOD测量需要5d,费时费力,不能及时反馈相关消息.因此,研究与开发方便、快速、准确的BOD测定仪受到了国内外学者的广泛关注.此文评述了BOD生物传感器快速测定仅的组成、性能、测定条件、响应速度等方面的国内外研究现状,分析了目前BOD传感器研究与开发中存在的问题和局限,并提出了今后进一步研究的方向.     

CellSense生物传感器毒性分析系统应用研究

介绍了一种基于大肠杆菌(Escherichia coli)的CellSense生物传感器毒性分析系统,对其毒性分析条件进行了初步优化,并探讨了其在重金属离子生物急性毒性分析中的应用性能.结果表明,以p-苯醌为电子传递介质时,基于大肠杆菌的CellSense生物传感器具有良好的毒性分析特性,测试得Hg2+、Cu2+、Zn2+和Ni2+四种金属离子的EC50分别为1.1、6.5、66.0和119.0μg·mL-1;呼吸基质的pH低于6.0和盐度(NaCl)高于3%(w/w)时,对E.coli的活性产生一定的抑制作用.     

Screening and identification of bacterial strain producing (R)-1-phenyl-1,2-Ethanediol by efficient whole-cell catalytic reduction system北大核心CSCD

(R)-1-Phenyl-1,2-ethanediol [(R)-PED] is an important chiral intermediate used to synthesize pharmaceutical intermediates. The production of (R)-PED is of great practical significance. A novel bacterial strain HBU-SI7 isolated from soil samples was screened using a-hydroxyacetophenone (2-HAP) as substrate, which is capable of enantioselective reduction of 2-HAP to (R)-PED. HBU-SI7 was identified as Rhodococcus sp. by bacterial morphological observation and 16S rDNA analysis. The analysis of whole-cell catalytic system showed that the bioconversion of 2-HAP reached 96.2% in the two-phase reaction system (dibutyl phthalate/phosphate buffer = 1/3, V/V) with the substrate concentration of 3.0 g/L, and 99.3% of e.e. for (R)-PED was achieved. A new enantio selective reduction system with the whole cells of Rhodococcus sp. was established for efficient production of (R)-PED, which has potential research and application values. © 2018 Science Press. All rights reserved.