排序方式: 共有92条查询结果,搜索用时 15 毫秒
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生物强化去除吡啶的特性及微生物种群动态变化分析 总被引:1,自引:1,他引:0
在接种活性污泥的序批式反应器中投加固定化吡啶降解菌Paracoccus sp.KT-5,强化吡啶的生物降解,并与未投加固定化微生物的反应器进行对照,通过末端限制性片段长度多态性(T-RFLP)分析手段,探讨了运行过程中生物强化与未强化反应器中微生物群落结构的动态变化,并探讨了生物强化反应器的去除效果.结果表明,投加固定化吡啶降解菌可以加速反应器的启动.当吡啶初始浓度为195.6~586.8 mg.L-1,随着反应器的运行,投加固定化菌株的强化作用并不明显;但当吡啶初始浓度为782.4~2 934 mg.L-1,投加固定化菌株显示出优势.T-RFLP分析结果表明,投加的固定化菌株KT-5作为优势菌始终存在于反应器的固定化生物相和悬浮生物相中. 相似文献
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酸性含硒含镉废水低温生物强化处理技术研究 总被引:1,自引:1,他引:0
本研究将枯草芽孢杆菌接种于活性污泥中,系统考察了酸性含硒含镉废水的低温生物强化处理效果.研究发现,活性污泥联合枯草芽孢杆菌对硒、镉的去除效率(Se:94.9%,Cd:99.1%)高于单一枯草芽孢杆菌(B菌)、混合枯草芽孢杆菌及活性污泥.影响因素试验结果表明,在20℃、pH 4.0、甲醇为碳源时生物强化硒、镉去除效率较高.利用SBR反应器处理酸性(pH 4.0)含硒(3.7~10.0 mg·L~(-1))含镉(5.0~11.2 mg·L~(-1))废水,在低温(8.0~10.8℃)条件下运行80个周期,硒、镉和COD的平均去除率分别为97.4%、90.7%和95.0%.红外光谱分析表明,酰胺基、芳香族C—H伸缩健在硒、镉去除中起到主要作用.透射电镜与能谱分析证实了生物强化污泥对硒、镉去除之后形成纳米颗粒物.通过高通量测序分析微生物群落结构,发现生物强化污泥中富集了Pseudomonas(假单胞菌属)、Dechloromonas(脱氯单胞菌)等典型硒还原菌. 相似文献
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Jun QIAO Chengdong ZHANG Shuiming LUO Wei CHEN 《Frontiers of Environmental Science & Engineering》2014,8(2):293-304
This study evaluated the effectiveness of different amendments--including a commercial NPK fertilizer, a humic substance (HS), an organic industrial waste (NovoGro), and a yeast-bacteria consortium--in the remediation of highly contaminated (up to 6% of total petroleum hydrocarbons) oilfield soils. The concentrations of hydrocarbon, soil toxicity, physicochemical properties of the soil, microbial population numbers, enzyme activities and microbial community structures were examined during the 90-d incubation. The results showed that the greatest degradation of total petroleum hydro- carbons (TPH) was observed with the biostimulation using mixture of NPK, HS and NovoGro, a treatment scheme that enhanced both dehydrogenase and lipase activities in soil. Introduction of exogenous hydrocarbon-degrading bacteria (in addition to biostimulation with NPK, HS and NovoGro) had negligible effect on the removal of TPH, which was likely due to the competition between exogenous and autochthonous microorganisms. None- theless, the addition of exogenous yeast-bacteria consor- tium significantly enhanced the removal of the aromatic fraction of the petroleum hydrocarbons, thus detoxifying the soil. The effect of bioaugmentation on the removal of more recalcitrant petroleum hydrocarbon fraction was likely due to the synergistic effect of bacteria and fungi. 相似文献
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概述了芳香族磺酸基有机物的废水处理技术,分析了各种技术的优点和存在的问题,对难降解有机物的处理进行了展望,提出具有优势的处理方法。 相似文献
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基因工程菌生物强化MBR工艺处理阿特拉津试验研究 总被引:11,自引:6,他引:5
以生活污水为共基质,考察了基因工程菌在MBR和活性污泥反应器中对阿特拉津的生物强化处理效果,以及生物强化处理对污泥性状的影响.结果表明,基因工程菌在MBR中对阿特拉津具有很好的生物强化处理效果,阿特拉津平均出水浓度为0.84 mg/L,平均去除率为95%,最大去除负荷可以达到70 mg/(L·d).生物强化的MBR对生活污水中COD的平均去除率为71%,COD平均出水浓度65 mg/L,COD容积负荷增加对COD去除效果有一定影响;对生活污水中的氨氮具有很好的去除效果,氨氮平均出水浓度为1.1 mg/L,平均去除率为97%,最大氨氮去除负荷为143 mg/(L·d).与普通MBR污泥相比,生物强化MBR污泥的硝化活性和亚硝化活性略高,碳氧化活性略低,因此表现出氨氮处理效果很好,COD处理效果略差.阿特拉津的存在会对污泥性状产生影响,可能是造成污泥碳氧化活性低的原因. 相似文献
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发酵生物制氢反应器的产氢菌生物强化作用研究 总被引:1,自引:0,他引:1
将发酵产氢菌Ethanoligenens sp. B49投加到连续流搅拌槽式反应器(CSTR)的活性污泥中,以糖蜜废水为底物,进行强化活性污泥产氢效能的研究.对生物强化前和生物强化后反应系统的产氢能力、发酵产物组成和pH值进行了对比分析.结果表明,在COD容积负荷为12 kg/(m3·d)条件下,投加产氢菌可显著提高反应系统的产氢能力并改善发酵产物组成.反应系统的比产氢速率从强化前的3.6 mmol/(kg·d)提高到强化后的5.7 mmol/(kg·d),是生物强化前的1.5倍.生物强化前,反应系统液相发酵产物乙醇、乙酸和丙酸的平均浓度分别为6.8、 5.3和4.8 mmol/L,生物强化后乙醇、乙酸和丙酸的平均浓度分别为10.5、 7.5和1.7 mmol/L,其中乙醇型发酵目的产物乙醇和乙酸在总发酵产物中的比例从生物强化前的72.0%提高为强化后的86.8%.生物强化作用使出水pH值从4.5~4.7下降为4.3.产氢菌的生物强化作用有助于反应器在低负荷运行期迅速形成产氢能力较高的乙醇型发酵. 相似文献
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The effectiveness of genetic bioaugmentation relies on efficient plasmid transfer between donor and recipient cells as well as the plasmid’s phenotype in the recipient cell. In the present study, the effects of varying organic carbon substrates, initial recipient-to-donor cell density ratios, and mixtures of known recipient bacterial strains on the conjugation and function of a TOL plasmid were tested in sterile soil slurry batch reactors. The presence of soil organic carbon was sufficient in ensuring TOL plasmid transconjugant occurrence (up to 2.1 ± 0.5%) for most recipient strains in soil slurry batch mating experiments. The addition of glucose had limited effects on transconjugant occurrence; however, glucose amendment increased the specific toluene degradation rates of some Enterobacteriaceae transconjugants in soil slurry. Initial cell density ratios and mixtures of recipient strains had smaller impacts on plasmid conjugation and resulting phenotype functionality. These observations suggest that genetic bioaugmentation may be improved by minimal altering of environmental conditions. 相似文献