DNA碱解旋速率法及其在DNA损伤研究中的应用

对DNA碱解旋速率法的细胞DNA经碱解旋后通过羟基磷灰石(HA)柱分离单、双链DNA时的洗脱次数、温度以及DNA回收效果进行了研究。结果表明,用0.125mol/L和0.25mol/L磷酸钾缓冲液各3ml,先后各洗脱一次,即可满足细胞DNA链断裂研究的要求。洗脱双链DNA时的温度为60℃即可。~3H-DNA与细胞其它组分一起通过HA柱时,~3H-DNA的回收率达94％左右。应用这一技术对电离辐射引起的中国田鼠肺细胞和小鼠脾细胞DNA单链断裂损伤与照射剂量的关系进行了探讨。结果表明,DNA单链断裂程度与照射剂量呈正相关。     

Stimulation of human amniotic fluid cell proliferation and colony formation by cell plating on a naturally produced extracellular matrix

Human amniotic fluid cells exhibit a higher cloning efficiency and rate of cell proliferation when maintained on dishes coated with a naturally produced extracellular matrix (ECM) in comparison with the regular tissue culture plastic. In 22 out of 31 amniotic fluid samples there was by plating the cells on ECM a 2–6 fold increase in number and size of colonies and in the cell density per colony as detected by actual staining and viewing of each colony. These effects yielded, in 21 of 41 additional samples, a reduction ranging from 2–8 days, in the culture time elapsing between amniocentesis and the first harvesting of cells for chromosomal analysis. An even greater effect was obtained with primary cells that failed to attach to plastic surfaces and stayed floating in the medium but did attach and proliferate when seeded on ECM. Cells that were left firmly attached to ECM after the first trypsinization and harvesting of cells for chromosomal analysis yielded colonies ready for second karyotyping in less than half the time required for cells maintained on plastic. Studies with secondary cultures of human amniotic fluid cells have demonstrated a 5–10 fold decrease in serum requirement of cells cultured on ECM as compared with plastic. Addition of fibroblast growth factor (FGF) to the cultures further potentiated the effects of ECM. The ECM induced stimulation of cell attachment and proliferation was not associated with any chromosomal anomalies, nor did it interfere with the handling procedure. ECM coated dishes may be useful to reduce the time interval between amniocentesis and diagnosis, in particular when the amniotic cells exhibit an exceedingly slow rate of proliferation on plastic or when large quantities of cells are required for enzymatic studies.     

对苯二甲酸复合降解生产单细胞蛋白的研究

研究通过降解对苯二甲酸的酵母和细菌的复合处理,使TA的降解率达到95%以上,并生产了单细胞蛋白。该单细胞蛋白营养价值高,经急性和遗传毒性试验表明属实际无毒类,并不具遗传毒性,是优良的单细胞蛋白。     

铝电解自焙糟烟号静电净化技术

应用电除尘与静电凝聚技术相结合,净化自焙阳极铝电解槽生产过程中所产生的有害气体和烟尘.有关部门测试认定,其沥青烟、氟化物和粉尘的排放量均低于国家排放标准.     

The use of amniotic FSH levels in resolving a discrepancy between fetal chromosomal and phenotypic sex

We report a case in which a discrepancy emerged between the prenatal diagnosis of female chromosomal sex and male sex at ultrasound examination. The FSH dosage performed on an amniotic fluid sample previously stored confirmed the male phenotype of the fetus. The effectiveness of the AF-FSH level dosage in prenatal diagnosis was taken into consideration.     

超声波技术在污泥处理中的研究及发展

本文分析了超声波降解有机物的机理 ,并对超声波技术在污泥处理中的研究现状进行了综述 ,同时提出可以利用超声波分解污泥作为形成微生物隐性生长的一种方法 ,并达到污泥减量的目的     

硒酸钠和亚硒酸钠对长春花细胞悬浮生长的影响

研究了硒化合物对长春花(C.roseus)细胞悬浮生长的影响.在pH5.5培养介质中,当Se(Ⅵ)>0.5ppm或Se(Ⅳ)>0.6ppm时,将抑制C.roseus细胞悬浮生长,其致死浓度分别是100ppm Se(Ⅵ)和2.0ppm Se(Ⅳ).C.roseus对硒化合物有效强的富集作用.硒化合物的毒性和培养介质的pH值有关.在本试验条件下,硒酸钠和亚硒酸钠对Hg²⁺无拮抗作用.     

Human chromosomes in prenatal diagnosis: A one step high resolution technique

A simple high resolution technique for human chromosomes is described for fibroblasts obtained from amniotic fluid cell cultures. The application and clinical significance of this technique in prenatal diagnosis is discussed.     

人工神经网络结构对径流预报精度的影响分析

建立了基于径流形成机理的以时段降水量与前期径流量为预报因子的前向多层人工神经网络径流预报模型；分析了网络结构对月径流预报精度的影响，发现随网络结构的复杂化，网络训练误差减小，模型评定的确定性系数增大，并均趋于稳定，预报检验的确定性系数总趋势是减小；发现影响模型精度的决定因素是网络输入单元数，亦即径流影响因素；提出了以模型评定与预报检验共同高效或等效的模型选择的折衷方法，以及按模型适宜预报域进行多模型组合预报的最佳预报域组合法。