应用流式细胞分析技术研究微纳尺度SiO2对雄性大鼠睾丸生精细胞的毒性作用

为了进一步研究纳米与微米尺度SiO2对雄性大鼠的生殖毒性作用,选择不同剂量的纳米SiO2(20～40nm)与微米SiO2(1～10μm),采用气管滴注方式对雄性Wistar大鼠分组染毒.于染毒5周后处死大鼠,应用流式细胞技术对睾丸生精细胞进行分析.结果表明:1)高、低剂量纳米SiO2组及高剂量微米SiO2组细胞凋亡率均显著高于对照组;2)与对照组相比,高、低剂量纳米SiO2组及高剂量微米SiO2组1C细胞显著减少,4C细胞显著增加;3)与对照组相比,高剂量纳米SiO2组和高剂量微米SiO2组G0/G1期细胞比例显著降低,高剂量纳米SiO2组G2/M期细胞比例显著增加.结果提示纳米SiO2能够阻滞细胞周期进程,诱导生精细胞凋亡;与微米SiO2相比,纳米SiO2对大鼠睾丸生精细胞的损伤有更严重的趋势.     

Cu2+对一种绿球藻生长及生理特性的影响

研究了不同浓度的Cu2 (0.01,0.1,1,10,50,100,200mg/L)对绿球藻(Chlorococcumsp.)生长、形态结构及生理特性的影响.结果表明,Cu2 对绿球藻的显微结构、生长及生理状态的影响比较显著.与对照BG11培养的绿球藻比较,0.01～1mg/LCu2 浓度下培养的绿球藻,细胞壁无明显增厚,色素没有多大变化,但蛋白核由一个变为多个;而在高浓度(10～200mg/LCu2 )下,细胞壁明显增厚为多层,色素减少,蛋白核减少并回复到1个或消失.低浓度Cu2 (0.01,0.1mg/L)对绿球藻生长无影响;高于0.1mg/L浓度时,绿球藻生长变得缓慢甚至出现负生长.在1mg/LCu2 浓度下,绿球藻对Cu2 的去除率最高为90.5%;叶绿素含量及光合作用强度随Cu2 浓度的升高逐渐降低;呼吸强度在0.01～10mg/LCu2 浓度范围内逐渐升高,大于10mg/LCu2 则逐渐降低.结果提示,绿球藻是一种耐受Cu2 胁迫的藻类,对其去除率也高,可以应用于含铜污水的处理.图3表3参17     

红豆杉培养细胞紫杉醇的分离及鉴定

红豆杉培养细胞紫杉醇的分离及鉴定甘烦远，郑光植，彭丽萍，沈月毛（中国科学院昆明植物研究所昆明６５０２０４）关键词红豆杉；紫杉醇；细胞培养；分离鉴定ＩＳＯＬＡＴＩＯＮＡＮＤＩＤＥＮＴＩＦＩＣＡＴＩＯＮＯＦＴＡＸＯＬＦＲＯＭＣＵＬＴＵＲＥＤＣＥＬＬＳＯＦ...     

Bisexually bonded ranging in chimpanzees (Pan troglodytes verus)

Abstact While male mammals seek to maximize access to potential mates, females maximize feeding efficiency. Ranging patterns are therefore often sex specific. Sex-specific ranging patterns have also been reported for East African chimpanzees and a recent study on female ranging patterns concludes that social organization is best described by a male-bonded community model, where females occupy individual home ranges that are distributed within the boundaries of the male-defended range. In West African chimpanzees, however, such sex-specific ranging patterns have not been consistently observed and a bisexually bonded community model, where both sexes use the entire home range equally, has been suggested to best describe social organization. In this study we analyze 5 years of data on individual ranging patterns of chimpanzees in the Taï National Park (Côte dIvoire) to test specific predictions of the different models of chimpanzee social organization. We found that although males in Taï had slightly larger home ranges than females, all individual home ranges and core areas overlapped highly. Small individual home range size differences were entirely due to the use of peripheral areas and were correlated with female social dominance. These findings strongly support the bisexually bonded community model for Taï chimpanzees. Thus, we conclude that there are fundamental differences in the space-use patterns of East and West African chimpanzees and discuss possible factors leading to such differences.Communicated by D. Watts     

固定化细胞技术应用于废水处理的研究进展

详细介绍了固定化细胞技术，综述了近年来固定化细胞的制备方法、载体的特性、固定化细胞的反应特性，及固定化技术在废水处理中的应用现状和情景，并指出了今后的研究方向。     

环境内分泌干扰物诱发小鼠睾丸细胞DNA损伤的研究

应用单细胞凝胶电泳技术技术，研究了烷基酚类化合物对离体小鼠睾丸细胞DNA的损伤作用。实验结果表明，9种烷基酚类化合物对小鼠睾丸细胞均能引起不同程度的DNA损伤，并呈现剂量效应关系，其高剂量组与对照组相比，均有极显著性差异（P＜0．01）。实验结果亦表明，其DNA的损伤程序与化学结构有一定的关系。     

多区蜂窝CDMA系统中的干扰及其抑制

在分析多区蜂窝ＣＤＭＡ系统干扰的基础上,提出了开,闭环结合的功控方式,要求和以载干比（ＣＩＲ）为基础的功控算法,从而抑制干扰,改善系统性能,增加系统容量。     

Human chromosomes in prenatal diagnosis: A one step high resolution technique

A simple high resolution technique for human chromosomes is described for fibroblasts obtained from amniotic fluid cell cultures. The application and clinical significance of this technique in prenatal diagnosis is discussed.