城市污水处理厂Candidatus Accumulibacter的菌群结构及定量分析

Candidatus Accumulibacter是污水生物除磷系统中的优势聚磷菌.本研究采用编码聚合磷酸盐激酶的功能基因(polyphosphate kinase 1gene,ppk1)作为遗传标记,对9个污水处理厂共12个活性污泥样品中Accumulibacter的进化枝水平的丰度及其菌群结构进行了研究.实时定量PCR(Quantitative PCR,QPCR)的结果显示,Accumulibacter的6个进化枝(IA、IIA、IIB、IIC、IID、IIF)均存在于12个污泥样品中.A2O及其改良工艺中总Accumulibacter丰度最高,达到总菌的22.77%.所有样品中Accumulibacter均以Type-II为主,其中,IIC、IID的丰度均达到了108cells·g-1(以MLSS计),占Accumulibacter的平均比例分别为75.34%和14.87%,是污水处理厂中的优势聚磷菌.分支IA丰度最低,占总Accumulibacter比例平均只有0.32%.系统发育分析结果显示,每个样品中Accumulibacter分支多样化,均含有IIA、IIC、IID分支.QPCR和系统发育分析都证实了生物除磷效果与Accumulibacter的群落结构密切相关,除磷效果不好可能和IID占总Accumulibacter比例较高有关.     

Investigation of affecting parameters on treating high-strength compost leachate in a hybrid EGSB and fixed-bed reactor followed by electrocoagulation–flotation process

In this research, treatability of high-load compost leachate in a hybrid expanded granular sludge bed (EGSB) and fixed-bed (FB) bioreactor followed by electrocoagulation–flotation (ECF) system was examined. The operational factors in EGSB–FB were influent chemical oxygen demand (COD), hydraulic retention time (HRT) and COD/nitrogen ratio (COD/N). And, their interactive effects on the efficiency of COD removal and biogas production rate (BPR) as responses were analyzed and correlated by response surface methodology (RSM). The optimum conditions of the hybrid EGSB–FB reactor were acquired at COD = 7800 mg/L, HRT = 35 h, COD/N = 70, in which COD removal efficiency was 83% and BPR 94 mL/h. The amount of confidence interval was 95%. COD (relevant coefficient = 9.8) and HRT (relevant coefficient = −24) were resulted respectively as the most effective parameters on COD removal and BPR. Yet, COD/N parameter imposed negative effect on COD removal and BPR in values less than about 100. The outcomes indicated that operated ECF as post-treatment in constant conditions (electrolysis time = 75 min, electrodes distance = 3 cm, voltage = 20 V) successfully satisfied discharge criteria in the most part of experimental domains.     

低温下强化水芹浮床对农村污水的深度处理研究

在低温条件下,利用强化水芹浮床对生物净化槽处理后的崇明岛农村生活污水进行深度处理.研究结果表明,水芹在浮床内保持着较好的生长状态,是一种优越的低温条件下浮床植物;强化水芹浮床由纤维填料和水芹交替分布组合而成,提高了浮床内的微生物浓度,强化了浮床对污染物质的降解作用,从而使其对有机质、氮、磷及悬浮物保持着较好的净化效果,平均出水浓度COD≤40 mg/L,NH4+-N≤10 mg/L,TN≤15 mg/L,TP≤1.0 mg/L.     

典型环境中三种自然环境加速试验方法的环境强化效果分析

目的研究自然环境加速试验方法的环境强化效果。方法通过分析实时监测获得的典型环境不同自然环境加速试验装置中,温湿度环境因素变化数据,找出典型环境中不同自然环境加速试验装置内部的温湿度环境因素变化规律,计算得到典型环境中不同自然环境加速试验方法对温湿度环境因素的强化效果。结果得到了典型环境中不同自然环境加速试验装置的特征温湿度变化规律曲线图。结论玻璃框、黑箱、强制通风玻璃框等3种自然环境加速试验装置对自然环境的光照热效应有明显的强化作用,其内部温度变化梯度大小与环境中太阳辐射量大小相关。     

Performance and microbial diversity of an expanded granular sludge bed reactor for high sulfate and nitrate waste brine treatment

The disposal of waste brines has become a major challenge that hinders the wide application of ion- exchange resins in the water industry in recent decades. In this study, high sulfate removal efficiency （80%-90%） was achieved at the influent sulfate concentration of 3600 mg/L and 3% NaC1 after 145 days in an expanded granular sludge bed （EGSB） reactor. Furthermore, the feasibility of treating synthetic waste brine containing high levels of sulfate and nitrate was investigated in a single EGSB reactor during an operation period of 261 days. The highest nitrate and sulfate loading rate reached 6.38 and 5.78 kg/（m3-day） at SO42--S/NO3-N mass ratio of 4/3, and the corresponding removal efficiency was 99.97% and 82.26% at 3% NaC1, respectively. Meanwhile, 454-pyrosequencing technology was used to analyze the bacterial diversity of the sludge on the 240th day for stable operation of phase X. Results showed that a total of 9194 sequences were obtained, which could be affiliated to 14 phyla, including Proteobacteria, Firmicutes, Chlorobi, Bacteroidetes, Synergistetes and so on. Proteobacteria （77.66%） was the dominant microbial population, followed by Firmicutes （12.23%） and Chlorobi （2.71%）.     

应对二氧化碳浓度上升问题的研究:CO_2的捕获、储存与利用

为了缓解日益严重的CO2浓度升高导致的温室效应等环境问题,针对各国CO2过量排放与温室效应逐渐加剧的现状,讨论传统CO2减排方法与CO2的捕获、储存与利用(CCSU)的优缺点和优势互补,论述针对大型CO2排放点的减排措施——CCSU的必要性。对CCSU进行系统的介绍和认识,并对CCSU所包含的各种捕获技术、储存技术以及利用技术的特点进行对比和分析,论述其适用范围、地域和经济因素。结合国外一些现有的应用CO2的先进技术和经验,对CO2的捕获与利用的联用作出分析和展望。尤其是CO2的化工利用具有广阔的研发和发展前景,CO2EOR(CO2提高采收率技术)在技术和经济上具有实际应用意义。为新型环保型能源体系的建设提供参考。     

Surfactant dissolution and mobilization of LNAPL contaminants in aquifers

Improper disposal, accidental spills and leaks of non-aqueousphase liquids (NAPL) such as gasoline, fuel oil and creosote result in long-term persistent sources of groundwater pollution.Column and 2-D tanks experiments were conducted to evaluate the use of surfactant-enhanced recovery of light non-aqueous phase liquids (LNAPL) in groundwater aquifers. These experiments focused on the use of surfactants to promote dissolution and mobilization in addition to evaluating the increase of aqueous phase permeability as residual NAPL is recovered. Further experiments are presented that show the innovative use of surfactants during primary pumping to recover free product canpotentially increase the amount of free product recovered, canpotentially reduce the amount of residual NAPL remaining afterprimary pumping and performs better than the use of surfactantsto mobilize trapped residual NAPL.     

微污染水处理技术研究进展

饮用水水源微污染日益严重,给传统的饮用水处理工艺提出新的挑战.本文阐述了饮用水处理技术的研究进展,包括:强化传统处理工艺、预处理技术、深度处理技术及应用,并给出了各种技术的优缺点.     

城市污水混凝强化处理中污泥的特性探讨

介绍了城市污水处理过程中产生污泥的特点及分类,目前城市污水化学强化一级处理中化学污泥的特性、处置方法以及资源利用等方面的研究成果.     

厌氧膨胀颗粒污泥床(EGSB)在高浓度工业废水处理中的应用

介绍了一种新型的用于高浓度有机废水处理的EGSB反应器 ,该反应器的工艺特点包括反应器内部的生物相的分层 ,颗粒污泥的变速膨胀 ,以及高效的三相分离装置等。该反应器最高CODCr容积负荷可达到 2 5kg m3·d以上 ,最高进水CODCr浓度可达 30 0 0 0mg L以上。并介绍了几个应用该工艺的具有代表性的工程实例。