Reproductive isolation between populations from Northern and Central Europe of the leaf beetle Chrysomela lapponica L.

Summary. Allopatric populations of the leaf beetle Chrysomela lapponica are known to feed upon either willow (Salicaceae) or birch (Betulaceae). This study aimed to elucidate the differentiation process of these allopatric populations. We investigated whether these allopatric populations specialized on different host plants are still able to produce fertile offspring when interbreeding. Individuals from a population in Finland (willow specialists) and one in the Czech Republic (birch specialists) were crossed in laboratory. Hybrid formation succeeded only between females from the Czech, birch specialized population and males from the Finnish, willow specialized population, while no eggs were produced by females of the willow specialists having mated males of the birch specialists. Behavioral, morphological, physiological, and chemical features of the F₁ hybrids were studied. The chemical composition of larval defensive secretion and feeding preferences of the resulting F₁ hybrids mainly showed similarities with the paternal phenotype, while the area of black coloring on the offspring’s elytra was intermediate between those of the parental elytra. F₁ hybrids did not accept the host plant (birch) of their mothers for feeding and only survived on willow. Thus, since mothers only lay eggs on birch, we found evidence for a postzygotic isolation mechanism between the individuals of the two investigated populations: when having been mated with willow specialized Finnish males, the birch specialized Czech mothers place the hybrid eggs on a plant species (birch), on which the hatching larvae cannot survive.     

Ability of Wildlife Overpasses to Provide Connectivity and Prevent Genetic Isolation

Abstract:  We reviewed research on wildlife overpasses in the context of their genetic effectiveness to provide connectivity between population patches that have been isolated by road construction. The potential ecological consequences of such habitat fragmentation include reduction of gene flow between subpopulations and hence an increase in genetic differentiation and a decrease in genetic diversity. Among the solutions to provide connectivity between patches isolated by roads, wildlife overpasses are one of the most expensive alternatives. Despite the high costs associated with their construction, most of the studies assessing their use by wildlife remain observational, reporting evidence for passage use but few data on the number of individual crossings. Moreover, the use itself of wildlife overpasses does not appear sufficient to assess their effectiveness from a genetic viewpoint because a minimum number of individuals is required to assure gene flow between population patches and because the spatiotemporal dimension of individual movements and demographic parameters of subpopulations must be considered. So far, there is no evidence that wildlife overpasses do or do not efficiently address genetic issues. This lack of data is probably due to the fact that few mitigation efforts have implemented monitoring programs that incorporate sufficient experimental designs into pre- and postconstruction evaluation. To assess the genetic effectiveness of wildlife overpasses, long-term monitoring programs, including fieldwork and genetic analyses, are needed.     

高效铜绿微囊藻溶藻菌WJ6的分离鉴定及溶藻特性

以铜绿微囊藻为研究对象,从富营养化水体分离了一株高效溶藻菌,通过分析形态学、生理生化特征及16S rDNA序列比对,鉴定了该溶藻菌株,分析了该菌株对铜绿微囊藻的溶藻方式、溶藻特性及其不同培养时期、不同浓度菌液及不同环境因子对溶藻效果的影响,并探讨了其可能的溶藻机制.研究表明：WJ6属于沙雷氏菌属（Serratia sp.GenBank登录号为KY462187）;溶藻菌WJ6以胞外释放溶藻物质为主直接溶藻为辅的溶藻方式;溶藻菌WJ6处于对数期时的溶藻率最高达87.50%;菌液浓度达1.4×10⁹CFU/mL以上,溶藻率最高为95.69%;在30℃、pH8条件下,溶藻率达90.00%;改良的基本培养基培养的溶藻菌溶藻率最高达98.50%;铵离子浓度大于2mol/L时,溶藻菌的溶藻率98.33%;当盐度为0.5%时,溶藻率较高为92.77%.溶藻菌株WJ6是1株高效溶铜绿微囊藻菌,在富营养化治理方面具有较好的应用前景.     

处理垃圾焚烧渗滤液的耐盐性优势菌筛选及特性

采用改良的Gibbons培养基从处理垃圾焚烧渗沥液的EGSB反应器中筛选分离获得3株具有耐盐性的COD降解优势菌（Strain1、Strain2和Strain3）,研究了优势菌的最佳生长条件;利用16S rDNA技术确定了优势菌的种属;采用静态实验方法研究了温度和接种量对优势菌COD降解性能的影响并通过菌种复配进一步提升COD降解效率;研究确定了Strain1（Bacillus属）、Strain2（Clostridium属）和Strain3（Enterobacter属）的最适生长条件。实验结果表明,3种优势菌在30~35℃时COD去除率最好。在Strain1和Strain2接种比例为1：2,进水COD为20 000 mg·L-1时,COD去除率达到54.3%,为最优接种组合。     

PCBs降解菌的筛选及其降解特性研究

采用富集培养的方法从多氯联苯(PCBs)污染土壤中筛选到1株高效降解PCBs的细菌,命名为PS-11.经16S rDNA初步鉴定,此菌株属于嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia).结果表明,菌株PS-11能够以PCBs作为唯一碳源生长并且能够降解PCBs,菌株PS-11对2 mg.L-1PCB52 4 d的降解率为31.1%,7 d的降解率可达52.9%;对难降解的高氯代多氯联苯PCB153 7 d的降解率为10.9%.此外,该菌株的环境耐受性比较好,在30℃、pH为7～9、PCB52浓度为2～10 mg.L-1的条件下生长较快、降解效果较好,尤其在pH 7、PCB52浓度为2 mg.L-1时降解率最高;碳源种类不同,菌株PS-11的生长能力也不同,其中在以蔗糖为碳源时PS-11的生长能力最强,以葡萄糖为碳源时PS-11的适应期最短,且蔗糖、葡萄糖和Tween-80均可提高PS-11对PCB52的降解率;同时菌株PS-11还可耐受一定浓度的重金属,其耐受性大小为Pb2+>Cd2+>Zn2+>Cu2+.     

应用形状记忆合金-橡胶复合支座的结构隔震

对橡胶支座存在的问题进行了分析，介绍了SMA-橡胶复合支座的基本构造和工作原理，建立了设置不同支座的单自由度结构的运动方程。应用大型有限元软件ANSYS对分别设置固定支座、普通叠层橡胶支座和形状记忆合金（SMA）-橡胶复合支座这3种不同支承条件的结构进行了，单向地震作用下的隔震仿真模拟，及节点位移和加速度的时程分析。分析表明，SMA-橡胶复合支座和普通叠层橡胶支座都能有效地减小结构的位移和加速度反应，而SMA-橡胶复合支座比普通叠层橡胶支座能更有效地减小结构的绝对位移反应，防止因隔震层位移过大而导致支座失稳。     

多株硝基苯降解菌的筛选

从长期受硝基苯严重污染的河道底泥和处理硝基苯废水的水解池沉积底泥以及生活污水处理厂消化池污泥中分离到18株可降解硝基苯并且利用硝基苯作为唯一碳源的细菌，从中筛选出5株对硝基苯降解能力较强的菌种，其中一株菌在pH8，温度35℃，硝基苯初始浓度32.98mg/L的液体培养基内培养7d。对硝基苯的降解率达85%。     

水泵系统噪声与振动控制

从水泵系统振动噪声传播途径控制的角度,着重分析了水泵机座二次隔振系统、水泵接管隔振、隔振系统位移限制、管道支架减振、管道穿墙隔振、管道阻尼隔声包扎等水泵系统振动固体传递途径的控制,消除与建筑结构之间的刚性连接。分析了振动噪声空气传播的阻隔与吸收。     

一株菲降解菌的鉴定及降解特性

从沈阳北部污水处理厂曝气池活性污泥中驯化和分离得到一株以菲为碳源的降解菌株W12,根据菌株形态和16S r DNA基因测序分析,该菌株鉴定为耳炎假单胞菌(Pseudomonas otitidis.).该菌株降解菲的最佳环境条件为:温度为30℃,p H值为7.0,摇床转速为170 r·min-1,接种量为10%,盐度为0.5%;菲初始质量浓度为1000 mg·L-1培养96 h后,降解率为65.80%,且对菲的最大耐受浓度为2000 mg·L-1;加入蛋白胨和酵母膏后,降解率分别提高到75.65%和70.85%.     

近20a三峡库区农林地利用变化图谱特征分析

土地利用变化图谱单元能够把表示"空间单元特征的图"与表示"时间发展之起点与过程的谱"合为一体,并建立起空间—属性—过程一体化数据信息。论文以TM/ETM影像为空间数据源,构建三峡库区1990—2000年和2000—2010年两个阶段农林地利用变化信息图谱,以及融合1990、2000、2010年这三个时期土地利用现状的变化模式图谱,以探讨库区近20 a来的农林用地变化轨迹和特征规律。结果表明:1 1990—2000年阶段的农林地利用变化图谱主要以农林地间及其和草地互换、建设用地占用农林地的图谱单元类型为主。空间差异上最为显著的是"耕地→建设用地(编码15)"、"草地→林地(编码32)"和"林地→草地(编码23)",空间分离度较低。2 2000—2010年阶段的农林地利用变化图谱单元分离度增大,农林地利用发生转换空间进一步扩张。最显著变化的是"耕地→林地(编码12)"和"耕地→建设用地(编码15)"图谱单元类型,其次是农林地转换为水域图谱类型(编码14和24)的面积增大。3库区农林地利用变化模式以前期变化型和后期变化型为主,反复变化型和持续变化型发生概率较小,库区农林地利用的转换具有明显的时间阶段性特征。