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81.
A 13.4 L biofilter treating an off-gas stream supplemented with methanol under two different situations was studied in terms of MeOH removal efficiency, microbial ecology and odor removal. During Period 1 (P1) the reactor was packed with wood bark chips with no pH control, treating an off-gas resulting from the aerobic chamber of a membrane biological reactor treating sewage and located outdoor, whereas during Period 2 (P2) a compressed air stream fed with MeOH was treated using PVC rings and maintaining pH at neutral values. Both systems operated at 96 g MeOH m−3 h−1 achieving removal efficiencies of around 90% during P1 and 99.9% during P2. The relative activity of biomass developed in both systems was assessed using respirometric analysis with samples obtained from both biofilms. Higher biomass activity was obtained during P2 (0.25-0.35 kg MeOH kg−1 VSS d−1) whereas 1.1 kg MeOH kg−1 VSS d−1 was obtained in the case of P1. The application of molecular and microscopic techniques showed that the eukaryotes were predominant during P1, being the yeast Candida boidinii the most abundant microorganism. A specific Fluorescence in situ hybridization probe was designed for C. boidinii and tested successfully. As a result of the neutral pH, a clear predominance of prokaryotes was detected during P2. Interestingly, some anaerobic bacteria were detected such as Desulfovibrio, Desulfobacteraceae species and also some archaea such as Methanosarcina. 相似文献
82.
污水中含磷物质主要来源于人类活动的排泄物及废弃物、工业合成洗涤剂等。本文分析了应用微生物除磷的环境影响因子,并对这一技术在我国的应用前景做出展望。 相似文献
83.
以萘为唯一碳源驯化长期被焦化废水污染的污泥,7周后,平板划线分离出两株黄杆菌FCN1,FCN2及一株短杆菌BCN1;并利用静态反应曝气及生物摇床试验对其降解菲、芘的效能进行了试验,同时研究了共基质及无机离子对它们降解菲、芘效果的影响.结果发现,BCN1对菲、芘的降解效果最好,淘米水对分离出的三株菌降解菲或芘均有明显的共代谢促进作用.苯酚、尿素、葡萄糖对两株黄杆菌降解菲有抑制作用.加入Fe3+使淘米水在两株黄杆菌降解菲的反应体系中的共代谢促进作用减小,但却增加了短杆菌反应体系中淘米水的促进作用. 相似文献
84.
微污染水源水生物处理中硝酸盐氮的变化 总被引:2,自引:0,他引:2
通过中试系统和大型工程 ,探讨了微污染水源水生物处理工艺中硝酸盐氮的变化规律。研究表明 ,微污染水源水生物处理工艺中硝酸盐氮的增加是氨氮生物硝化的结果 ;处理系统启动中硝酸盐氮变化率的变化反映了两类硝化细菌在生长速率和转化能力上的协调关系以及生物膜的成熟过程 ,启动结束时硝酸盐氮变化率趋于 1.0 0 ;稳定运行阶段各工况下处理系统硝酸盐氮变化率均在 1.0 0附近 ;水源水中少量的有机氮和亚硝酸盐氮对氨氮硝化过程无明显影响。硝酸盐氮变化率是描述微污染水源水生物处理系统氨氮硝化状况的重要参数。 相似文献
85.
86.
甲醇残液生化处理系统中好氧菌特性研究 总被引:1,自引:0,他引:1
以甲醇残液生化处理系统中的生物污泥为材料,运用分离技术,获得好氧菌30株。研究了每株细菌的生理生化特性和培养特征,将30株细菌鉴定为9个属,并进一步探讨了甲醇残液生物降解的模式。 相似文献
87.
Stin OC Carnahan A Singh R Powell J Furuno JP Dorsey A Silbergeld E Williams HN Morris JG 《Environmental monitoring and assessment》2003,81(1-3):327-336
Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish. 相似文献
88.
89.
90.
优势菌对焦化废水中几种有机物降解特性的实验研究 总被引:3,自引:0,他引:3
从焦化厂曝气池活性污泥和废水处理工艺出水中筛选出6株革兰氏阳性优势菌,根据菌株、菌落形态和大量生化实验将菌株鉴定到属.确定了降解所需的最佳实验条件.分别为温度30 ℃,转速100 r/min,菌株投加量20%(V(菌液)/V(废水)).实验发现,筛选出的6株优势菌对喹啉、吡啶和萘有良好的降解效果,但以喹啉为碳源和氮源筛选出来的H24,H26和H31对喹啉的去除率优于其他3株菌株,而以萘为唯一碳源的H14对萘的去除率优于其他菌种.6株优势菌的驯化活性污泥能进一步提高对喹啉、吡啶和萘的去除能力,去除率在90%以上. 相似文献