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61.
水稻精细胞差异表达基因RSG6的克隆和抗体制备 总被引:3,自引:2,他引:3
选用在水稻精细胞和二细胞花粉的差减文库中获得的在精细胞中表达量显著增强且可能代表新基因的EST之一 (BF4 75 2 0 7)为探针 ,筛选水稻精细胞cDNA文库 ,得到一全长为 1176bp的序列 ,其开放读码框编码 2 81个氨基酸 ,与已知蛋白质无明显同源性 ,属于一新发现的基因 ,GenBank登录号为AF4 4 2 4 90 .这是第 1次从高等植物精细胞中分离到的全长基因 .RT PCR结果证明该基因在根、叶、二细胞花粉、成熟花粉、授粉子房和精细胞中均有表达 ,但在精细胞中的表达量要高得多 ,是精细胞差异表达基因 .将此基因命名为RSG6 (ricespermgene6 ) .将RSG6的编码区克隆到表达载体pQE30上 ,构建重组质粒 .经IPTG诱导后 ,在E .coliM15 (pREP4 )中表达出了N端融合了 6×His的融合蛋白 .用纯化的融合蛋白免疫家兔 ,制得高效价、高特异性的抗体 相似文献
62.
SINGH R KUMAR V SHARMA S BEHL RK SINGH BP NARULA N 《应用与环境生物学报》2005,11(6):751-755
ThepracticaluseofAzotobacterchroococcumasbiofertilizer hasbeenincreasinglyidentifiedinrecentyears.Oftentheefficacy ofthesebacteriaisassessedonthebasisofcropresponseswithout knowingthesurvival,persistenceandcompetitiveabilityofthein troducedstrain.Inoculat… 相似文献
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64.
We present a strategy for using an empirical forest growth model to reduce uncertainty in predictions made with a physiological process-based forest ecosystem model. The uncertainty reduction is carried out via Bayesian melding, in which information from prior knowledge and a deterministic computer model is conditioned on a likelihood function. We used predictions from an empirical forest growth model G-HAT in place of field observations of aboveground net primary productivity (ANPP) in a deciduous temperate forest ecosystem. Using Bayesian melding, priors for the inputs of the process-based forest ecosystem PnET-II were propagated through the model, and likelihoods for the PnET-II output ANPP were calculated using the G-HAT predictions. Posterior distributions for ANPP and many PnET-II inputs obtained using the G-HAT predictions largely matched posteriors obtained using field data. Since empirical growth models are often more readily available than extensive field data sets, the method represents a potential gain in efficiency for reducing the uncertainty of process-based model predictions when reliable empirical models are available but high-quality data are not. 相似文献
65.
红海榄(Rhizophora stylosa)属于乔木红树植物,它对不同盐度的生理反应存在着差异。本文通过对拒盐红树植物红海榄Rs在盐胁迫下各种生理反应的研究,分析了各种生理现象与盐度之间的关系,这对红树植物耐盐机理的研究打下了基础,并为中国南海岸耐盐红树树种的选育提供科学依据,也有助于从多学科研究的角度评估盐胁迫的生态重要性。通过对Rs用不同的盐度的水3个月的处理,发现Rs根、茎、叶中蛋白质质量分数随盐度的变化趋势与可溶性总糖质量分数与盐度的变化趋势相反。当盐度高于40时,Rs茎、叶的膜脂质过氧化破坏显著加强,植物体内超氧化物歧化酶(SOD)活性也明显增加,两者具有很好的相关性(R2=0.893)。随着盐度的升高,Rs各器官过氧化物酶(POD)和过氧化氢酶(CAT)活性都增强。根据实验结果,Rs能够在高盐度(50)下存活3个月。在盐度为20~30时,Rs在生理生态上表现对盐环境的适应性。当盐度高于40时,Rs的生理表现较为敏感,膜脂质氧化破坏严重。 相似文献
66.
用哌嗪和二硫化碳反应合成氨基二硫代甲酸中间体,再与二卤代烃反应得到1,4-双(二硫代酯基)哌嗪聚合物,考察了中间体和螯合树脂对Ag^ ,Cu^2 ,Zn^2 ,Ni^2 ,CO^2 ,Pb^2 ,Pd^2 ,Cr^3 的静态吸附性能,结果表明,两者均可以吸附金属离子,缩聚后的螯合树脂具有更强的吸附能力。 相似文献
67.
Regina?H.?F.?MacedoEmail author Mariana?O.?Cariello Jeff?Graves Hubert?Schwabl 《Behavioral ecology and sociobiology》2004,55(3):213-222
Guira cuckoos, Guira guira, exhibit a rare polygynandrous reproductive system with groups containing several male and female breeders, allowing for important tests of reproductive skew models. Female reproductive strategies involve leaving the group, varying clutch size, egg ejection and infanticide, among others. Here we examined the predictions of reproductive skew models relative to reproductive partitioning among females in groups. We used yolk protein electrophoresis to identify individual females eggs in joint nests. We found that reproductive partitioning favors early-laying females, which lay and incubate more eggs than females that begin laying later. Because the female that lays first tends to switch between repeated nesting bouts, and females do not always contribute eggs to each bout, female reproductive success tends to equalize within groups over time. The pattern of reproductive partitioning differs from that described for anis, another crotophagine joint-nester. We calculated reproductive skew indices for groups in 2 years, for both laying and incubation, as well as an overall population value. These were compared to random skew generated by simulations. Varying degrees of skew were found for different groups, and also across sequential nesting bouts of the same groups. Overall, however, skew did not deviate from random within the population. Nests that reached incubation tended to have lower skew values during the laying phase than nests terminated due to ejection of all eggs followed by desertion. Groups had higher reproductive skew indices in their first nesting bout of the season, and these nests frequently failed. These results illustrate the importance of social organization in determining not only individual, but group success in reproduction, and highlight the flexibility of vertebrate social behavior.Communicated by J. Dickinson 相似文献
68.
职业接触苯乙烯工人静脉血中蛋白质环氧苯乙烯加合物的分析测定 总被引:1,自引:0,他引:1
静脉血中蛋白质-环氧苯乙烯(SO)加合物的分析测定是将蛋白质中半胱氨酸残留经分解后,用Raney Ni催化反应生成两种加合物:α-苯己醇,β-苯已醇,再用五氟苯氯衍生,GC/MS测定,用同样的方法测SD大鼠血的蛋白质加合物,结果表明动物血中蛋白质加的与实验的苯乙烯或SO剂量有较好的相关性;而且SO与半胱氨酸的反应α位强于β位,在人群实验中,对一个石棉厂职业接触苯乙烯工人静脉血中的蛋白质加合物进行测 相似文献
69.
Trotta F Cravotto G Casile G 《Environmental science and pollution research international》2003,10(3):144-146
Chiral, atropisomeric 2,2'-dihydroxy-1,1'-binaphthyl has been extensively used to direct asymmetric processes. Its key role in asymmetric catalysis has spurred efforts to synthesize it in the optically pure form, but the reported synthetic routes have a significant environmental impact. In an aqueous peroxydase-cyclodextrin system the oxidative coupling of 2-naphthol took place very rapidly in almost quantitative yield and resulted in an enantiomeric excess. This one-pot synthesis do not require any organic solvents and oxidising metal cations. 相似文献
70.