海洋微藻活体及乙醇固定状态下基因组DNA的微量提取方法

探讨了海洋微藻活体及固定状态下基因组DNA的提取方法,即作者改进的CTAB法.结果表明,CTAB改进法提取的基因组DNA蛋白质、酚、盐和小分子的污染较少,多糖成份也得到了有效去除.用5%乙醇固定样本提取DNA的效果最好,对原生动物抑制效果也较明显;10%以上的乙醇可杀死原生动物,但对DNA的降解率较大;30%以上的乙醇对细菌抑制较明显,但DNA都有较大程度的降解.本法提取的DNA可用于正常的酶切和PCR.用甲醛、甲醇/冰醋酸等其它固定液固定样品,DNA的得率低或者质量和纯度低.图7表1参27     

低浓度硝基苯标准样品的考核

提出了在四级站的考核项目中，在考核低浓度硝基苯标准样品时通过改进：曲线绘制方法、取过滤液方法、调pH值方法、标样稀释方法等手段提高考核结果的准确性。     

Elemental analysis of some Egyptian ores and industrial iron samples by neutron activation analysis

ＩｎｔｒｏｄｕｃｔｉｏｎＮｏｗａｄａｙｓ,ｍｏｒｅｉｎｔｅｒｅｓｔｈａｓｇｒｏｗｎｉｎｔｈｅｄｅｔｅｒｍｉｎａｔｉｏｎｏｆｔｈｅｅｌｅｍｅｎｔａｌｃｏｎｓｔｉｔｕｅｎｔｓｏｆｍｏｓｔｉｎｄｕｓｔｒｉａｌｍａｔｅｒｉａｌｓｓｕｃｈａｓｉｒｏｎｗｈｅｒｅｓｅｖｅｒａｌｄｉｆｆｅｒｅｎｔｍｅｔｈｏｄｓａｒｅｕｓｅｄ(Ｈａｓｓａｎ ,1982 ;Ａｂｄｕｌ,1990 ;Ｆａｉｌｅｙ ,1979;Ａｎｄｅｒｓｏｎ ,1981) .Ｒｅｃｅｎｔｌｙ ,ｔｈｅｒｅｗｅｒｅｍａｎｙｉｎｖｅｓｔｉｇａｔｉｏｎｓｏｎｅｌｅｍｅｎｔａｌａｎａｌｙｓｉ…     

运用回归分析测算水口镇商业用地基准地价

惠州市惠城区水口镇原隶属于惠阳市,2003年惠城区行政区划进行了战略性调整,惠阳撤市设区,水口镇划归惠城区管辖, 这样水口镇现执行的基准地价标准(原惠阳标准)需要更新.目前基准地价的评估方法有很多种,通过建立样点地价与土地定级网格单元总分值关系模型,运用回归分析来测算水口镇商业用地基准地价.     

137Cs in marine samples from the Brazilian southeastern coastal region

The present work presents the results of ¹³⁷Cs concentration in seawater, fish and sediments samples collect in 11 sampling points, crossing the Brazilian Southeastern coastal region, from Vitória (ES) to Santos (SP), on a routine basis from 1997 to 2002. This monitoring program was carried out by the Instituto de Radioproteção e Dosimetria (IRD/CNEN/MCT), in cooperation with the Instituto de Estudos do Mar Almirante Paulo Moreira (IEAPM/Brazilian Navy), aiming at determining artificial radionuclides in marine samples. Additionally to the ¹³⁷Cs results, ⁹⁰Sr concentrations in fish samples from 1998 to 2002 are also reported.The ¹³⁷Cs in seawater follows a lognormal distribution, with a geometric mean of 1.8 Bq m^-3 and a geometric standard deviation of 1.4 (n=54), decay corrected to June/2002. For ¹³⁷Cs levels in fish samples a geometric mean of 0.19 Bq kg⁻¹ and a geometric standard deviation of 2.9 (n=39), decay corrected to June/2002, with a range of 0.03 to 1.7 Bq kg⁻¹, were obtained. Based on the ¹³⁷Cs mean concentration in fish as well as in seawater, a transfer factor of 1 × 10² was calculated, which is quite in agreement with the recommended value found in the Safety Report Serie 19.     

For What Applications Can Probability and Non-Probability Sampling Be Used?

Almost any type of sample has some utilitywhen estimating population quantities. The focus inthis paper is to indicate what type or combination oftypes of sampling can be used in various situationsranging from a sample designed to establishcause-effect or legal challenge to one involving asimple subjective judgment. Several of these methodshave little or no utility in the scientific area buteven in the best of circumstances, particularlycomplex ones, both probabilistic and non-probabilisticprocedures have to be used because of lack ofknowledge and cost. We illustrate this with a marbledmurrelet example.     

挥发酚水质样品保存条件的研究

应用正交试验法探讨挥发酚水质样品的最优保存条件，找出了对挥发酚水质样品保存影响最大的因素为保存温度，其次为样品的pH值。经实验证明，挥发酚样品浓度为0.2mg/L，温度5℃，保存时间在8h内，pH值为4的条件下，挥发酚水质样品的保存效果最好。     

环境中长寿核素的ICP-MS测量研究及进展

本文综述了ICP-MS用于环境样品中U,Np和Pu的放射性长寿命核素测定的各项相关技术以及近年来一些重要的研究和进展状况,同时对技术难点——干扰效应进行了讨论.     

环境标准样品在国家地表水采测分离工作中的应用

按照《国家地表水环境质量监测网监测任务作业指导书(试行)》中实验室分析测试的质量保证和质量控制要求,结合江苏省南京环境监测中心的实际工作,综合分析2017年10月—2018年8月间国家地表水采测分离工作中环境标准样品在样品测试准确度验证、检测人员能力确认、检测设备期间核查及关键化学试剂检查等方面的应用情况。为保障环境监测结果量值溯源的统一性、准确性、一致性和可比性,提出丰富水环境标准样品的浓度水平、解决标准样品供需不平衡问题、改善消耗量大的标准样品操作方法、建立环境标准样品使用信息共享平台等实践建议,为合理提升环境标准样品应用效能、有效保证水环境监测数据质量提供参考。     

Plant Uptake/Bioavailability of Heavy Metals from the Contaminated Soil after Treatment with Humus Soil and Hydroxyapatite

Cellulose nitrate membrane filter was used for the preconcentration-separation of Cu, Co, Cd, Pb and Cr ions. The analyte ions were collected on the membrane filter by the aid of carmine. Then membrane filter was dissolved by using nitric acid. The levels of the analytes in the final solutions were determined by flame atomic absorption spectrometry (FAAS). The analytical parameters including pH, amounts of carmine, sample volumes etc. have been optimized. No influences have been observed from the matrix ions. The detection limits for analytes were in the range of 0.08 μg/l-0.93 μg/l. The validation of the procedure was checked by the analysis of standard reference sediment (GBW 07309). The present method has been successfully applied for the FAAS determinations of analyte ions in real samples including black tea and magnesium salts.