蛋白酶对水环境中病毒的灭活作用

以大肠杆菌噬菌体T₄作为模式病毒,研究了蛋白酶对病毒的灭活作用.试验结果表明:蛋白酶灭活病毒的效果明显. 适宜条件下,67.5u/mL的蛋白酶K处理1h对纯水中病毒(6.2×10⁵PFU/L)和生活污水中病毒(7.2×10⁵PFU/L)灭活率分别达到了99.4%和49.4%,处理3h的灭活率分别是>99.9%和81.1%;工业蛋白酶1398在75.0u/mL酶浓度下处理1h对纯水水中病毒(1.7×10⁵ PFU/L)灭活率达到74.4%.pH和温度对病毒灭活效果的影响不明显.     

基于社会燃烧理论的突发公共卫生事件网络情绪传播模型

为有效引导和管控突发公共卫生事件网络舆情,考虑社会相关性和网络群体间情绪状态转移的不确定性,基于社会燃烧理论研究突发公共卫生事件网络情绪传播机制.首先基于社会燃烧理论分析网络用户群体的社会影响因素,建立未燃-阴燃-燃烧-抑燃-稳定(UDBFS)网络情绪传播模型和考虑干预措施的网络情绪传播模型;然后以新冠肺炎(COVID...     

新冠疫情期间无锡市环境空气质量分析

通过对无锡市区环境空气污染物的连续监测,对新冠疫情期间环境空气质量进行研究分析.结果表明,2月疫情期间,无锡市环境空气质量同比与环比均有明显好转,AQI指数在24～80,均为优良天.这可能与全社会生产、生活、活动有极大变动有关,可指导空气污染防治工作的努力方向.从单项指标看,除O3以外的其他5项指标均有不同程度的好转....     

环境对阿尔茨海默病病因影响

阿尔茨海默病是一种发生于老年人群的原发生退行性脑病。目前，关于ＡＤ病研究中的中心问题是弄清楚其发病的病理，如果可以抑制的一步和几步，将减缓或防止其发病，因此搞清ＡＤ病的发病机理对预防和治疗ＡＤ病相当重要。     

虚拟蜜罐技术在网络安全中的应用研究

面对网络上复杂而多变的黑客攻击,提出利用虚拟蜜罐技术主动吸引黑客的攻击,来监视和跟踪入侵者的行为并进行记录,进而研究入侵者所使用的攻击工具、攻击策略和方法。笔者论述了虚拟蜜罐技术的基本概念,通过3方面的应用实例,较为系统地研究了虚拟蜜罐技术在网络安全中的应用,证明虚拟蜜罐技术可以有效收集攻击信息,保护实际工作网络,并讨论了虚拟蜜罐的优点和风险。     

Intergrated approach for microbiological impact assessment of public wastewater treatment plants

Wastewaters are a source of pathogenic micro-organisms in the environment. The microbial load and residues found in the final effluents of wastewater-treatment plants (WWTPs) depend on the WWTPs' abatement capacity and the final disinfection treatment systems applied to wastewaters before discharge into water. A historical database with data on surface and marine-coastal water quality and on the characterization of WWTP effluents was made using data from 1997 to 2004 to assess the microbiologic impact along the coast of the Venice province (Italy, northern Adriatic sea). The monitoring of river and sea discharges along the coast is integrated with the application of the Synthetic Pluriennal Faecalization Index (ISPF). The experimental study was conducted in the period from November 2002 to April 2004 by the Veneto Regional Environmental Prevention and Protection Agency. The results of this investigation on faecal contamination together with previous data are presented with a preliminary performance characterization of the WWTPs' disinfection technologies (sodium hypochlorite, peracetic acid, UV rays, and ozone).     

Increased Infectious Disease Susceptibility Resulting from Outbreeding Depression

Abstract:  The mechanisms by which outbreeding depression leads to reduced fitness are poorly understood. We considered the hypothesis that outbreeding can depress fitness by increasing the susceptibility of hybrid individuals and populations to infectious disease. Competitive breeding trials in experimental ponds indicated that outbred largemouth bass (  Micropterus salmoides ) crossed from two geographically and genetically distinct populations suffered a reduction in fitness of approximately 14% relative to parental stocks. We measured the comparative susceptibility of these same outbred stocks to a novel viral pathogen, largemouth bass virus. Following experimental inoculation, F2 generation hybrids suffered mortality at a rate 3.6 times higher than either F1 generation hybrids or wild-type parental fish. Analysis of viral loads indicated that viral replication was more rapid in F2 fish than in F1 hybrids or wild-type parental fish. We attribute these results to the disruption of coadapted gene complexes in the immune systems of outbred fish in the F2 generation. Increased susceptibility to infectious disease may be an important but underappreciated mechanism by which outbreeding reduces the fitness of individuals and populations and by which novel infectious diseases emerge in populations of hybrid organisms.     

纳米TiO2-光催化灭活水中噬菌体MS2

提出了一种简易的水中病毒灭活方法,以噬菌体MS2为模式病毒,探讨了紫外光照射时间、TiO2浓度、紫外光强度对自来水中MS2灭活效率的影响;评价了太阳光代替紫外光用于光催化灭活病毒的可行性.研究结果表明,聚乙烯(PE)材质的样品袋能更好地透过紫外光;紫外光照射6h,50mg/L的TiO2可以灭活自来水中7.95Log的MS2;紫外光强度在45μW/m2以下时,灭活率随强度的提高而增加;在溪水中,50mg/L的TiO2仅有4.87Log的灭活效率. 在太阳光下,50mg/L的TiO2在自来水和溪水中对MS2的灭活效率差异不明显,都达到了6.1Log以上,表明太阳光-TiO2灭活水中的病毒可行且高效.     

我国棉铃虫病毒杀虫剂的研究开发和应用

棉铃虫核型多角体病毒（ＮＰｖ），从分离开始，经过实验室研究到工厂生产商品销售，至今历经了１８个春秋。是中国生防史上第一次使一种昆虫病毒的研究，从技术上的成功转变成商业上的开发成功。从１９８８～１９９３年，总共生产棉铃虫病毒杀虫剂１００ｔ，使用面积７万ｈａ，（１００多万亩）。在防治棉花、烟叶、辣椒和蕃茄等作物上的棉铃虫和烟青虫方面，均获得了有效的控制。本文主要介绍该病毒的基本特性，生产工艺和应用效果，并展望未来的前景。     

云南省环境监测中心站网络安全对策及应用

根据近年国内网络领域的新技术,结合本单位网络建设的需求及工作经验,从防火墙、入侵检测、防病毒、漏洞扫描、访问控制等几个方面作了阐述,对如何建设一个安全的网络提出了自己的见解。