固定化根霉脂肪酶的性质及在手性酯拆分中的应用

通过载体筛选及固定化过程优化,选用硅胶25℃吸附4h,1%戊二醛交联1h的方法对根霉（Rhizopus sp.Bc0-09m01)脂肪酶进行了固定化,对固定化酶的各种酶学性质进行的研究表明,酶的最适作用温度为40－45℃,是适作用pH为7．5,在4－30 ℃范围中性偏酸（pH4.0-9.0)环境中比较稳定,固定化酶的热稳定性及pH稳定性较游离酶有较大幅度的提高,将固定化酶用于拆分环氧丙醇丁酸酯,在转化率达到53％时得到的残留底物对映体过量值（ees)可达90％,固定化酶批式反应连续运行10批后酶活仍保持80％。     

（R）—酮基布洛芬乙酯对映选择性水解酶产生菌的筛选及其特性

以酮洛芬乙酯为唯一碳源,经过两轮富集培养从土壤中分离得到45株优先生成（S）-酮洛芬和25株优先生成（R）-酮洛芬的菌株,其中产物对映体过量值高于85%的分别有13株和9株。在25株优先选择（R）构型的菌株中,G13号菌的活性较高而且选择性最好,对G13号菌的培养和催化特性研究表明：在培养基中添加吐温-80可以显著提高细菌的催化活力;静息细胞的最适反应温度为40℃,最适pH范围为7.0-8.0,在具有档板和磁力搅拌的三角板中,用G13号菌的静息细胞水解酮洛芬乙酯（50mmol/L）,68h的转化率为33.7%,产物（R）-酮洛芬的对映体过量值达到93%。     

Misanthropy Without Borders: The International Children's Rights Regime

The issue of children's rights has become key to human rights-based international security strategies. The UN Convention on the Rights of the Child (1989) is being operationalised in complex political emergencies. Children's rights now inform humanitarian principles. Universal concern for children is viewed as transcending political and social divides and able to mobilise societies to confront social problems and prevent war. The operationalisation of child rights is accompanied by the development of psycho-social programmes to rehabilitate the child victim. Critically analysing the implications of the children's rights regime for the right to self-determination, the paper unpacks the assumptions underlying children's rights and psycho-social intervention. The paper begins by examining the conceptualisation of the rights-holding subject universalised under the UN Convention and then goes on to consider Article 39 on the right to psycho-social intervention. Equally important as the novel conceptualisation of childhood and children's rights under the international children's rights regime is the (unspoken) mistrust of adulthood and political rights that informs the imperative to institutionalise children's rights as higher law. Moreover while the rights-based approach consciously sought to move away from the earlier moralising child-salvation model, psycho-social rehabilitation reveals a similar preoccupation with deviancy, but conducted through the paradigm of psychological functionalism. Rather than representing a trend towards more humane international relations, the paper suggests that the elevation of children's rights is premised on a profound disenchantment with humanity. The logical implication of the international children's rights regime is to challenge both the moral and political capacity of individuals and their right to self-determination and to institutionalise a more unequal international system.     

Work–family conflict and neck and back pain in surgical nurses

Objective. Surgical nurses’ work is physically and mentally demanding, possibly leading to work–family conflict (WFC). The current study tests WFC to be a risk factor for neck and lower back pain (LBP). Job influence and social support are tested as resources that could buffer the detrimental impact of WFC. Methods. Forty-eight surgical nurses from two university hospitals in Germany and Switzerland were recruited. WFC was assessed with the Work–Family Conflict Scale. Job influence and social support were assessed with the Copenhagen Psychosocial Questionnaire, and back pain was assessed with the North American Spine Society Instrument. Results. Multiple linear regression analyses confirmed WFC as a significant predictor of cervical pain (β?=?0.45, p?β?=?0.33, p?=?0.012). Job influence and social support did not turn out to be significant predictors and were not found to buffer the impact of WFC in moderator analyses. Conclusion. WFC is likely to affect neck and back pain in surgery nurses. Work–life interventions may have the potential to reduce WFC in surgery nurses.     

基于高分遥感的危险化学品重大危险源安全布局动态管控研究*

为遏制危险化学品重特大安全事故,解决传统安全监测手段的空间局限性问题,结合高分遥感与数值模拟技术,开展危险化学品重大危险源区域安全布局动态管控研究。利用数值模拟技术模拟并确定危险化学品重大危险源重点管控范围;基于高分遥感影像提出适用于危险化学品重大危险源安全布局动态管控的变化检测方法,动态监测安全防护范围内的建筑、道路等人工构筑物的变化情况,严控事故影响范围内的人工构筑物密度;以内江市隆桥化工为实例开展示范应用。结果表明:高分遥感技术能够高效监测危险化学品重大危险源安全防护范围内的工程建设情况,有助于危险化学品企业保持足够的外部安全防护距离,降低危险化学品重特大安全事故风险。     

从监测角度对环境污染治理设施存在问题及运行模式的认识

文章论述了在点源治理模式下,环境污染治理设施易存在的问题及其产生原因,并据此提出解决问题的对策及运行模式.     

废线路板粉末中二噁英含量的测定

采用加速溶剂萃取-柱层析-高分辨质谱法检测废线路板粉末样品中17种2,3,7,8-PCDD/Fs的含量。采用TRDIOXIN-5MS毛细管色谱柱(60 m×0.25 mm×0.25μm)进行分离和多离子检测(MID)模式进行检测,以保留时间和同位素特征离子丰度比进行定性,用13C标记同位素稀释内标法进行定量。结果表明,17种2,3,7,8-PCDD/Fs化合物的标准曲线的线性相关性良好,线性相关系数均大于0.999 9,该方法分析的提取内标的平均回收率范围为37.25%~82.75%,RSD8%,样品中各异构体的加标回收率为112.66%~124.26%,RSD7%。应用该方法检测废线路板粉末样品,回收率在58.67%~123.00%范围内,1,2,3,4,6,7,8-Hp CDF、1,2,3,4,6,7,8-Hp CDD、OCDF、OCDD的质量比分别为2.74、2.50、5.57、16.37 pg/g,TEQ分别为0.027 4、0.025 0、0.005 6、0.016 4 ng/kg。     

Investigating the Fate and Transport of Escherichia coli in the Charles River,Boston, Using High‐Resolution Observation and Modeling1

Abstract: The processes affecting the fate and transport of Escherichia coli in surface waters were investigated using high‐resolution observation and modeling. The concentration patterns in Boston’s Charles River were observed during four sampling events with a total of 757 samples, including two spatial surveys with two along‐river (1,500 m length) and three across‐river (600 m length) transects at approximately 25‐m intervals, and two temporal surveys at a fixed location (Community Boating) over seven days at hourly intervals. The data reveal significant spatial and temporal structure at scales not resolved by typical monitoring programs. A mechanistic, time‐variable, three‐dimensional coupled hydrodynamic and water quality model was developed using the ECOMSED and RCA modeling frameworks. The computational grid consists of 3,066 grid cells with average length dimension of 25 m. Forcing functions include upstream and downstream boundary conditions, Stony Brook, and Muddy River (major tributaries) combined sewer overflow (CSO) and non‐CSO discharge and wind. The model generally reproduces the observed spatial and temporal patterns. This includes the presence and absence of a plume in the study area under similar loading, but different hydrodynamic conditions caused by operation of the New Charles River Dam (downstream) and wind. The model also correctly predicts an episode of high concentrations at the time‐series station following seven days of no rainfall. The model has an overall root mean square error (RMSE) of 250 CFU/100 ml and an error rate (above or below the USEPA‐recommended single sample criteria value of 235 CFU/100 ml) of 9.4%. At the time series station, the model has an RMSE of 370 CFU/100 ml and an error rate of 15%.     

The Decision Analysis Interview Approach in the Collaborative Management of a Large Regulated Water Course

There are always conflicting goals in the management of large water courses. However, by involving stakeholders actively in the planning and decision-making processes, it is possible to work together toward commonly acceptable solutions. In this article, we describe how we applied interactive multicriteria decision analysis (MCDA) in a collaborative process which aimed at an ecologically, socially, and economically sustainable water course regulation policy. The stakeholders' opinions about the alternative regulation schemes and the relative importance of their impacts were elicited with the HIPRE software. Altogether, 20 personal interactive decision analysis interviews (DAIs) were carried out with the stakeholders. Our experience suggests that the DAIs can considerably improve the quality and efficiency of the collaborative planning process. By improving communication and understanding of the decision situation in the steering group, the approach helped to develop a consensus solution in a case having strong conflicts of interest. In order to gain the full benefits of the MCDA approach, interactive preference elicitation is vital. It is also essential to integrate the approach tightly into the planning and decision-making process. The project's home pages are available to the public at http://www.paijanne.hut.fi/.     

Cutting the cake: Supporting environmental fund allocation decisions

This paper describes a decision support model for allocating financial resources amongst multiple user groups in environmental management problems. The model is based on the multiple criteria analysis (MCA) method of compromise programming. It was used to inform the allocation of Natural Heritage Trust funds across 14 regions in Queensland, Australia. The model targets funding to those regions with greater natural resource management needs. Need is determined by 19 weighted criteria relating to natural resource assets and threats. The model was accepted by the Australian Government, Queensland Government and regional groups as an appropriate means for allocating program funds; first in 2005 and then again, with improvements, in 2007. This paper shows that an MCA model can improve the transparency, auditability and acceptance of allocation decisions which would otherwise be heavily politicised.