多层级系统安全状态量化及动态评价方法

为及时、合理评价生产系统安全状况,促进现场安全管理工作的持续改进,依据生产现场日常隐患排查的安全管理机制,将反映生产现场系统安全属性的安全要素集,根据行业特点进行归类来提取评价指标,形成一个表达系统安全状态的逻辑结构,研究以诸多安全要素风险值来量化评价指标的方法,用以刻画系统安全逻辑结构中指标层的安全状态。采用适用于多样本关联分析的熵权法,客观、动态地确定指标权重;采用加权灰色关联分析法,描述评价样本接近于系统安全目标的程度来确定评价等级,实现了生产现场系统安全动态、定量评价。实例分析表明,通过系统安全状态量化与动态评价能及时甄别反映系统薄弱环节的系统安全主控因素,为现场安全管理提供决策依据。     

Microbial characterization of nitrification in a shallow, nitrogen-contaminated aquifer, Cape Cod, Massachusetts and detection of a novel cluster associated with nitrifying Betaproteobacteria

Groundwater nitrification is a poorly characterized process affecting the speciation and transport of nitrogen. Cores from two sites in a plume of contamination were examined using culture-based and molecular techniques targeting nitrification processes. The first site, located beneath a sewage effluent infiltration bed, received treated effluent containing O₂ (> 300 µM) and NH₄⁺ (51–800 µM). The second site was 2.5 km down-gradient near the leading edge of the ammonium zone within the contaminant plume and featured vertical gradients of O₂, NH₄⁺, and NO₃⁻ (0–300, 0–500, and 100–200 µM with depth, respectively). Ammonia- and nitrite-oxidizers enumerated by the culture-based MPN method were low in abundance at both sites (1.8 to 350 g^− 1 and 33 to 35,000 g^− 1, respectively). Potential nitrifying activity measured in core material in the laboratory was also very low, requiring several weeks for products to accumulate. Molecular analysis of aquifer DNA (nested PCR followed by cloning and 16S rDNA sequencing) detected primarily sequences associated with the Nitrosospira genus throughout the cores at the down-gradient site and a smaller proportion from the Nitrosomonas genus in the deeper anoxic, NH₄⁺ zone at the down-gradient site. Only a single Nitrosospira sequence was detected beneath the infiltration bed. Furthermore, the majority of Nitrosospira-associated sequences represent an unrecognized cluster. We conclude that an uncharacterized group associated with Nitrosospira dominate at the geochemically stable, down-gradient site, but found little evidence for Betaproteobacteria nitrifiers beneath the infiltration beds where geochemical conditions were more variable.     

PGD for monogenic disorders: aspects of molecular biology

Preimplantation genetic diagnosis (PGD) for monogenic diseases has known a considerable evolution since its first application in the early 1990s. Especially the technical aspects of the genetic diagnosis itself, the single-cell genetic analysis, has constantly evolved to reach levels of accuracy and efficiency nearing those of genetic diagnosis on regular DNA samples. In this review, we will focus on the molecular biological techniques that are currently in use in the most advanced centers for PGD for monogenic disorders, including multiplex polymerase chain reaction (PCR) and post-PCR diagnostic methods, whole genome amplification (WGA) and multiple displacement amplification (MDA). As it becomes more and more clear that when it comes to ethically difficult indications, PGD goes further than prenatal diagnosis (PND), we will also briefly discuss ethical issues. Copyright © 2008 John Wiley & Sons, Ltd.     

基于生态位与约束CA的土地资源优化配置模型研究——以武汉市黄陂区为例

土地资源优化配置一直是土地科学的核心问题之一。传统的土地资源优化配置模型主要关注土地利用的经济属性而忽略土地利用行为的生态环境效应．已不适应可持续发展的要求；并且在优化内容上都是对土地资源数量结构的优化。忽视了土地资源空间布局优化．近几年很多学者通过与相关模型算法的集成形成的优化模型从一定程度上对数量结构优化模型进行了改造。实现了社会、生态、经济三效益的优化配置目标。但将土地资源数量结构优化与空间布局优化系统研究的模型却非常少见。本文运用生态位模型对土地资源数量结构进行优化进而将数量优化结果作为CA模拟的约束条件对空间布局进行优化．实现了土地资源数量结构优化与空间布局优化的统一。     

PCDFs的Ah受体结合能力的定量结构效应关系

应用单苯环氯取代方法取G₁～G₁₀10个分子结构描述符,通过正向逐步线性回归方法构建对于Ah受体结合能力的定量结构-性质相关(QSAR)模型.与有关文献相比,此模型能更好地预测多氯代二苯并呋喃(PCDFs)竞争结合Ah受体logEC₅₀值,各自变量间不存在共线性.模型可以较好地反映PCDFs分子Ah受体结合能力与其分子氯取代位点之间的关系.     

补贴对林业生产及森林生态效益影响的经济学分析:一个定量分析模型

不同的林业补贴产生不同的效果:可能提高社会福利,也可能降低社会福利。出现这种差别的原因是补贴使林业生产收益流发生了变化,从而使得林业生产收益最大净现值到来的时间相对于社会最优时间提前或延后。论文建立了一个以林木吸收碳来衡量森林生态效益的林业生产收益定量模型,并通过该模型对华东某市的一项林场补贴政策进行分析。分析结果是,在没有补贴的情况下,该林场最优砍伐时间为第19年,当存在补贴的情况下,林场的最优砍伐时间提前到第11年,而随着碳价格从0增加至1000元/t,社会最优砍伐时间从第19年增加到第31年,造成的社会净损失从7750元/hm²增加到17704元/hm²。鉴于此,合理的林业补贴要能够促使私人效益与社会效益相统一。     

有机磷农药对乙酰胆碱酯酶的抑制作用及QSAR研究

以碘化硫代乙酰胆碱为底物,二硫代二硝基苯甲酸为显色剂,采用分光光度法测定了21种有机磷农药对乙酰胆碱酯酶(AChE)的半抑制浓度(IC₅₀).运用多元线性回归分析以及参数间的自相关分析建立有机磷农药对AChE抑制作用的QSAR模型.模型分析表明,有机磷农药对AChE的抑制作用主要受特定方向上的原子极化率及原子的电拓扑状态的影响,是多因素综合作用的结果.模型可解释高达78%的数据变异和显著的预测能力(q²=0.653),表明有机磷农药对AChE的抑制作用可借助结构活性关系模型进行预测与评价.