Interspecific mating of the introduced bumblebee <Emphasis Type="Italic">Bombus terrestris</Emphasis> and the native Japanese bumblebee <Emphasis Type="Italic">Bombus hypocrita sapporoensis</Emphasis> results in inviable hybrids

The bumblebee Bombus terrestris is not only an effective pollinator, but also a potential invasive alien species outside its native range. Recently, nearly 30% of queens of the Japanese native species Bombus hypocrita sapporoensis and B. hypocrita hypocrita were estimated to copulate with B. terrestris males in the field, suggesting that indigenous bumblebees could be genetically deteriorated through hybrid production with the introduced species. In this study, we evaluated hybrid production between the introduced B. terrestris and the indigenous B. hypocrita sapporoensis under laboratory conditions. The hatching rate of eggs derived from interspecific matings was 0% and 8.6% depending on the direction of the cross, which was significantly lower than that from intraspecific matings of B. terrestris (76.9%) and B. hypocrita sapporoensis (78.9%). Genetic studies using microsatellite markers revealed that both haploid and diploid individuals were present in the egg stage, whereas all hatched larvae were haploid. In addition, histological studies revealed that eggs derived from interspecific matings terminated development 2 days after oviposition. These results strongly suggested that eggs derived from interspecific matings are inviable due to post-mating isolation mechanisms. Mass release of exotic pollinators could cause serious population declines of native bumblebee species.     

Evaluating unintended consequences of intentional species introductions and eradications for improved conservation management

Increasingly intensive strategies to maintain biodiversity and ecosystem function are being deployed in response to global anthropogenic threats, including intentionally introducing and eradicating species via assisted migration, rewilding, biological control, invasive species eradications, and gene drives. These actions are highly contentious because of their potential for unintended consequences. We conducted a global literature review of these conservation actions to quantify how often unintended outcomes occur and to elucidate their underlying causes. To evaluate conservation outcomes, we developed a community assessment framework for systematically mapping the range of possible interaction types for 111 case studies. Applying this tool, we quantified the number of interaction types considered in each study and documented the nature and strength of intended and unintended outcomes. Intended outcomes were reported in 51% of cases, a combination of intended outcomes and unintended outcomes in 26%, and strictly unintended outcomes in 10%. Hence, unintended outcomes were reported in 36% of all cases evaluated. In evaluating overall conservations outcomes (weighing intended vs. unintended effects), some unintended effects were fairly innocuous relative to the conservation objective, whereas others resulted in serious unintended consequences in recipient communities. Studies that assessed a greater number of community interactions with the target species reported unintended outcomes more often, suggesting that unintended consequences may be underreported due to insufficient vetting. Most reported unintended outcomes arose from direct effects (68%) or simple density-mediated or indirect effects (25%) linked to the target species. Only a few documented cases arose from more complex interaction pathways (7%). Therefore, most unintended outcomes involved simple interactions that could be predicted and mitigated through more formal vetting. Our community assessment framework provides a tool for screening future conservation actions by mapping the recipient community interaction web to identify and mitigate unintended outcomes from intentional species introductions and eradications for conservation.     

Impact of bias in predicted height on tree volume estimation: A case-study of intrinsic nonlinearity

Bias originating from intrinsic nonlinearity in nonlinear models is caused by excess curvature in the solution locus of parameter estimates derived from least squares procedures. Bias due to intrinsic nonlinearity varies according to sample size as well as model specification. This paper analyses consequences of fractionising data into smaller sub-samples. Based on measurements of stem diameter and total tree height from the first Danish national forest inventory, it is demonstrated how data splitting at random may cause the intrinsic nonlinear curvature to exceed the critical F-value. Application of a Taylor-series expansion shows that, for all practical purposes, the bias in predictions of individual tree volume (based on stem diameter and tree height) is negligible. To minimize residual variance, intrinsic curvature and, in turn, prediction bias, it is recommended that data be stratified according to site conditions, stand characteristics or other relevant criteria. Finally, the preferred model should exhibit close-to-linear behaviour.     

新型固定床生物膜反应器硝化性能的研究

本文以无纺布作为硝化反应器的填料 ,对其硝化负荷能力进行了探讨 ,同时对硝化过程中亚硝酸盐氮积累和影响氨氮去除的原因进行了分析。在NH3 N容积负荷为 1 .7kg/(m3·d)的情况下 ,可实现 98%以上的NH3 N去除率。溶解氧浓度为 4mg/L ,进水氨氮浓度为 30 0mg/L时硝化柱内开始出现亚硝酸盐氮积累 ,通过加大曝气量等措施可在一定程度上消除其影响。停止运行两个半月后 ,可在一周内基本恢复硝化功能。     

Monitoring the formation of trihalomethanes in the effluents from a shrimp hatchery

Formation of trihalomethanes (THM) was monitored at the Laboratório de Camarões Marinhos (LCM) from the Universidade Federal de Santa Catarina. THM could be present because chlorinated effluents from disinfection are discharged from the different hatchery rooms. THM quantification was done through an analytical methodology using Purge&;Trap coupled with a gas chromatograph equipped with an electron capture detector. Relative standard deviation (RSD), limit of detection (LOD) and limit of quantification (LOQ) for the methodology corresponded to the ranges of 8–17%; 0.01–0.03 μg L^?1 and 0.03–0.08 μg L^?1, respectively. Linear working range was of 0.1–8.0 μg L^?1 for all compounds. Enrichment and recovery method was applied to evaluate possible matrix effects and the results varied from 71.2% to 107.9%. LCM was monitored between August and December, 2004. This study showed that THM did not increase with the increase in postlarvae production and also that the aquatic life and the surrounding environment were not affected.     

Factors affecting river health and its assessment over broad geographic ranges: the Western Australian experience

AusRivAS is an Australia-wide program that measures river condition using predictive models to compare the macroinvertebrate families occurring at a river site with those expected if the site were in natural condition. Results of assessment of 685 sites across all major rivers in Western Australia are presented. Most rivers were in relatively natural condition in the northern half of the state where the human population is low and pastoralism is the major land use. In the south, where the human population is higher and agriculture is more intensive, rivers were mostly more disturbed. AusRivAS assessment produced some erroneous results in rivers of the south-west cropping zone because of the lack of appropriate reference site groups and biased distribution of sampling sites. Collecting low numbers of animals from many forested streams, because of low stream productivity and samples that were difficult to sort, also affected assessments. Overall, however, AusRivAs assessment identified catchment processes that were inimical to river health. These processes included salinisation, high nutrient and organic loads, erosion and loss of riparian vegetation. River regulation, channel modification and fire were also associated with river degradation. As is the case with other assessment methods, one-off sampling at individual sites using AusRivAS may be misleading. Seasonal drought, in particular, may make it difficult to relate conditions at the time of sampling to longer-term river health. AusRivAS has shown river condition in Western Australia is not markedly different from other parts of Australia which, as a whole, lacks the substantial segments of severely degraded river systems reported in England.     

外来生物入侵关键点控制管理体系——基于风险管理的研究

将风险管理理论与外来生物入侵风险分析相结合，借鉴食品质量控制中的关键点控制原理，提出了防止外来生物入侵风险的关键点控制方法。针对人类有意引种、随人类活动无意引入以及自然传入3类外来生物入侵的传入途径，在入侵途径上确定若干个风险控制关键点，有针对性地提出了相应的控制措施，以减小外来生物的入侵风险，提高我国在防止外来生物入侵方面的管理与控制效率。     

环境水样前处理技术的探讨

环境水样前处理对监测结果准确度的影响不容忽视,是保证监测结果准确度的一个重要环节,样品前处理技术方法及需要注意的问题是保证监测结果真正可靠的保障。     

超临界流体萃取法

介绍了超临界流体的性质：超临界流体萃取法的原理，特点及应用。讨论了应用超临界流体萃取进行样品预处理的条件选择：超临界流体的选择，列举了数种常用的超临界流体，详尽介绍了ＣＯ２的特性及其在样品预处理中的应用范围；萃取条件选择的预测方法，试验方法，萃取压力的选择，， 同类别物质的以，不联机及联机的收集技术等。     

固体废物样品全溶解方法的比较研究

采用容器敞口酸消解、高压釜密闭酸消解及微波辅助酸消解3种溶样技术,对电镀污泥、尾矿渣、铬渣、砷钙渣、铅锌渣以及粉煤灰等固体废物试样的全溶解效果进行了对比试验,结果表明:敞口酸消解方法难以达到全溶解,繁琐耗时,易产生二次污染;高压釜密闭酸消解法溶解效果较好,但耗时和不安全;微波辅助酸消解法溶解效果最好,还具有快速方便,较少二次污染等特点。