Comparative study of polyhydroxyalkanoates production from acidified and anaerobically treated brewery wastewater using enriched mixed microbial culture

The production of polyhydroxyalkanoates(PHA) from wastewaters using microbial mixed cultures(MMC) has been attracting increased interest because of PHA's biodegradability characteristics. Production of PHA by an MMC enriched with PHA-accumulating bacteria was compared using anaerobically treated and acidified brewery wastewaters under various feeding strategies, namely pulse and batch feed addition. To obtain an enriched MMC, a sequencing batch reactor was inoculated with activated sludge fed with acetate and subjected to aerobic dynamic feeding. The enriched MMC was able to accumulate PHA up to 72.6% of cell dry weight(CDW) with pulse addition of acetate controlled by the dissolved oxygen(DO) concentration in the reactor. In a batch accumulation experiment with acetate,the PHA content achieved(28.5% CDW) was less than that of the pulse feeding strategy with the same amount of acetate(～2000 mg C/L). Using anaerobically treated and acidified brewery wastewater fed in pulses, the maximum PHA accumulated by the enriched MMC was similar for both wastewaters(45% CDW), in spite of the higher volatile fatty acid concentration in acidified brewery wastewater. The pulse feed addition controlled by the DO concentration was difficult to implement for wastewater as compared to acetate because the difference in DO concentration between substrate availability and depletion was low. For the batch addition of acidified wastewater, a slightly lower PHA content(39%CDW) was obtained. These results show that both brewery wastewaters can be utilized for PHA production with a similar maximum PHA storage capacity.     

完全混合式曝气系统运行特性及微生物群落结构解析

利用某污水处理厂完全混合式曝气系统处理生活污水,探讨了系统同步硝化反硝化脱氮过程,解析了系统中污泥微生物群落结构.中试试验结果表明,系统运行稳定,且在无外加碳源的条件下,COD、氨氮和总氮平均去除率分别为93.2%、96.9%和75.2%,出水COD、氨氮和总氮均优于一级A排放标准.系统污泥具有较强的反硝化能力,其脱氮速率是污水处理厂污泥的2.86倍以上.通过对系统污泥周质硝酸盐还原酶聚合酶链式反应(polymerase chain reaction,PCR)扩增和高通量测序结果,发现系统中存在好氧反硝化菌,污泥中优势菌为动胶菌属(Zoogloea)、陶厄氏菌属(Thauera)和Dechloromonas菌属.     

游离氨(FA)协同曝气时间对活性污泥沉降性能的影响

采用3个序批式反应器(SBR)(R0:硝化结束时停曝气;R_(0-30):硝化结束提前30 min停曝气;R_(0+30):硝化结束延迟30 min停曝气),控制3种游离氨(FA)浓度梯度(0.5,5.1,10.1 mg/L)协同3种曝气时间(t0:硝化结束时停曝气;t0-30:硝化结束提前30 min停曝气;t0+30:硝化结束延迟30 min停曝气)的条件下,研究了FA协同曝气时间对活性污泥沉降性能的影响。结果表明:整个试验过程,在初始FA浓度相同条件下,R0和R_(0+30)系统NH+4-N平均去除率分别为98.6%和99.3%,而R_(0-30)系统NH+4-N平均去除率仅为72.3%。在较低FA浓度(0.5,5.1 mg/L)条件下,随着曝气时间增加,活性污泥的污泥沉降比(SV30)值和污泥体积指数(SVI)值均逐渐降低,污泥沉降性能趋好。在较高FA浓度(10.1 mg/L)条件下,随着运行周期的增加,污泥沉降性能逐步变好,R_(0-30)系统的SVI平均值最大,R_(0+30)系统次之,R0系统最小,其值分别为165.1,152.5,134.5 m L/g,且污泥活性f平均值大小顺序fR_(0-30)>fR0>fR_(0+30),其值分别为0.77、0.70和0.65。这表明在较高FA浓度(10.1 mg/L)条件下,曝气时间延长,导致污泥活性降低。     

高浓度难降解工业废水菌种筛选及其降解特性

使用富集、稀释涂平板的方法从制药、染料、化工及精细化工、食品等多家工厂的混合高浓度难降解化工废水中筛选到8株细菌,经16S rDNA鉴定为Bacillus aquimaris、Enterobacter、Pseudomonas putida、Microbacterium、Agrobacterium、Alpha proteobacterium、Planococcus rifietoensis,其中两株细菌为Bacillus aquimaris。对各单一菌种生长条件的研究表明,各菌种生长的最适pH为7,最适接种量为15%（v/v）,最适生长温度为37℃,其中Pseudomonas putida的最适生长温度为30℃。在最适生长条件下,研究了各单一菌种及不同组合的混合菌种对废水的降解效果,结果表明在单一菌种中Pseudomonas putida对废水的降解效果最优,混合菌的降解效果优于单一菌种的降解效果,混合菌可将废水COD由1 249 mg/L降至97 mg/L,比普通活性污泥对废水的去除率提高了14.7%。     

钢铅组合防屈曲支撑的滞回性能与参数研究

为简化防屈曲支撑的加工工艺,提高防屈曲支撑的初始刚度和在小变形下的耗能能力,基于现有防屈曲支撑在截面形式与构造方式上的特点,提出了一种新型钢铅组合防屈曲支撑并进行了构造设计。通过有限元数值模拟,分析了钢铅组合防屈曲支撑的耗能特性与效果,建立了恢复力简化模型,并根据理想弹塑性材料本构关系推导出滞回规则。通过对不同设计参数的理论分析和数值模拟,分析了钢铅屈服力比、铅剪切面长宽比、核心段宽厚比和耗能段长度等参数对防屈曲支撑滞回性能的影响。研究结果表明,钢铅组合防屈曲支撑能够提供较大的抗侧刚度,耗能效果良好,加工工艺简单,适合工程应用。     

The emergence of the transition movement in Canada: success and impact through the eyes of initiative leaders

ABSTRACT

Originating in the UK in 2006, the Transition movement is oriented to local grassroots citizen-led efforts that prepare for and support a societal energy transition to a low-carbon future in response to climate change, peak oil, ecological degradation, and economic instability. Overlapping significantly with relocalization, degrowth/slow growth, local food, and related movements, and based on permaculture principles and a distributed network model, it embraces the opportunity to turn crisis into an opportunity to build more resilient, convivial, and vibrant local communities, declaring that “if it’s not fun, it’s not sustainable”. The Transition approach has spread rapidly around the world, including initiatives in over 100 communities and cities in Canada. This paper reports on the methods and results of a Canadian community-based research study aimed at understanding how and where the movement has taken root across the country, what Transition practice looks like, challenges and opportunities encountered, and lessons learned that could be applied within the movement and by others interested in the role of citizen-led initiatives for sustainability transition. Utilising a practice theory lens, drawing on an extensive web-scan of the movement’s online presence, a survey and interviews with initiative (co)founders, an e-survey of Transition members/participants, regional “structured story-dialogue” workshops, and key informant interviews, and informed by input from a Movement Advisory Group, we describe the research process and explore what success and impact mean to those most active in the movement.     

混合交通条件下行人左侧通行的安全性研究

听觉是人接受外部刺激的重要信息通道,然而实验表明,行人依靠听觉察觉身后危险的能力很弱,在道路混合交通条件下容易出现行人未及时发现身后驶来的车辆而引发交通事故。由于我国实行车辆靠右侧通行制度,因此,在目前机动车行驶噪声日益受到严格控制的情况下,让行人在混合交通的道路上靠道路的左侧通行,可以使其始终与距离自己最近的车辆保持相互面向,从而充分利用行人视觉对道路危险的良好感知能力,增强行人躲避危险的主动性,减少行人与车辆之间的危险冲突及事故发生。     

装备环境适应性的定量化表征技术探讨

环境适应性的定量化表征技术对装备环境适应性的论证、考核、评价具有十分重要的意义,由于定量表征技术的复杂性,至今没有形成统一的方法。依据环境适应性的定义、内涵,将环境适应性定量表示为环境适应度,定义了环境适应度的概念,说明了环境适应度各计算参数的含义,并以算例的方式对环境适应度的计算进行了说明。     

类球红细菌利用混合底物光合产氢特性研究

研究了类球红细菌(Rhodobacter sphaeroides)利用有机酸和糖类作为混合底物的光合产氢特性。初步研究了6种有机酸和3种糖类的产氢活性,并选用具有最大底物转化效率的琥珀酸钠和最大平均产氢速率的葡萄糖混合进行光合产氢,对底物利用和pH变化对产氢影响进行了探索。结果表明,类球红细菌利用混合底物可提高平均产氢速率、底物转化效率和底物利用率,琥珀酸钠和葡萄糖按15/15(mmol/L)混合最大平均产氢速率达19.1mL/(L·h),底物转化效率和底物利用率分别提高了35.3%和13.3%。混合底物利用中的pH值调节和底物利用率提高是有利于类球红细菌的氢气产生提高的重要因素。