植物铜耐性机理的研究进展

主要从植物根细胞壁积累固定、细胞膜对铜的吸收控制、金属配体的螯合作用、铜在系统液泡的分隔机制及胁迫蛋白的合成5个方面,分别阐述植物对铜分子的耐性机制的研究进展,全面了解了铜在植物中的亚细胞分布、铜在植物根系到地上部分运输过程的转运机制以及植物在铜胁迫下的抗性反应等。并在此基础上提出了存在的问题以及今后研究的重点。     

Application of ultra-sonication,acid precipitation and membrane filtration for co-recovery of protein and humic acid from sewage sludge

A novel method was applied to co-recover proteins and humic acid from the dewatered sewage sludge for liquid fertilizer and animal feed. The proteins in sewage sludge were first extracted using the processes of ultra-sonication and acid precipitation, and then the humic acid was recovered via membrane filtration. The extraction efficiency was 125.9 mg humic acid?g⁻¹VSS volatile suspended solids (VSS) and 123.9 mg proteins?g⁻¹ VSS at the optimal ultrasonic density of 1.5 W?mL⁻¹. FT-IR spectrum results indicated that the recovered proteins and humic acid showed similar chemical characteristic to the natural proteins and humic acid. The acidic solution (pH 2) could be recycled and used more than 10 times during the co-recovery processes. In addition, the dewatered sludge could be easily biodegraded when the humic acid and proteins are extracted, which was essential for further utilization. These findings are of great significance for recovering valuable nutrient from sewage sludge.     

Heat shock proteins in Daphnia detected using commercial antibodies: description and responsiveness to thermal stress

It has previously been shown that the level of Daphnia proteins detected by commonly used anti-HSP antibodies, with molecular weights corresponding to those of heat shock proteins, can be modified by various kinds of stress, including the threat of predation. In this study, we demonstrate for the first time that these proteins change their expression in response to thermal stress. Thus, it is concluded that Daphnia heat shock proteins, as components of the cellular stress defense, are involved in both, abiotic and biotic stress.     

DNA甲基化结合蛋白

DNA甲基化是哺乳动物细胞中最重要的表观遗传学修饰之一,大约70%—80%的CpG发生这种甲基化修饰.异常的甲基化在许多癌症中频发,启动子CpG岛的高甲基化作为普遍的失活机制介导抑癌基因沉默.甲基化信号由甲基化结合蛋白来转译,它们能够特异性识别并结合至甲基化位点通过募集辅阻遏复合物例如组蛋白去乙酰化酶(Histone Deacetylase,HDAC)等建立沉默的染色质,从而在DNA甲基化和基因沉默中起桥梁作用.目前,哺乳动物中已鉴定出的甲基化结合蛋白有三类,分别是:MBD(Methyl-CpG-Binding Domain)、Kaiso以及SRA(Set and Ring finger-associated)家族.本文就这三大家族(以MBD为主)各自的结构、功能、结合甲基化DNA的特性以及它们在某些疾病发生中的作用做一综述.     

Effect of the water-soluble fraction of diesel exhaust particles on the development and protein patterns of pollen grains in bean plants (Phaseolus vulgaris L.)

Excessive air pollution may adversely affect plant growth and development. The overall objective of this research was to elucidate some microscopic effects of diesel exhaust particles (DEP) on the formation, development, and structure of pollen grains and its proteins in Phaseolus vulgaris L. Bean plants were grown in experimental pots and treated with different concentrations of the water-soluble fraction of DEP. Flowers and young buds were removed, fixed in FAA₇₀ (formalin?:?acetic acid?:?ethanol, 2?:?1?:?17), and subjected to developmental studies. Our results show that DEP cause some abnormalities during pollen development. Pollen grains in normal plants are spherical in equatorial view and triangular in polar view; in DEP-treated plants, they were changed into irregular shapes in equatorial view and asteroidal in polar view. Delay in degeneration of the tapetum layer of the anther, and formation of giant, irregular, and non-fertile pollen grains are other results of DEP treatment. Gel electrophoretic studies revealed that a new protein with a molecular mass of 80?kD was formed in the DEP-treated group. Some pollen proteins may act as allergens, considering the fact that pollen allergy frequency is increased in polluted areas; the possibility arises that DEP could be an effective agent entail formation of detoxifying proteins which, on the other hand, can also act as allergens.     

Analysis on relationship between stress and drill cutting weight using numerical modeling - A case study in Jinjiazhuang coal mine

The relationship between stress and drill cutting weight (DCW) is studied with numerical modeling and field testing. Jinjiazhuang coal mine 110403 workface is used as a case study. Stress analysis of the workface is carried out with numerical modeling, with input parameters obtained from laboratory experiments. The stress of workface is examined at one section along the mining direction in terms of the stress distribution. Comparing simulating results of stress and field testing drill cutting weight, the stress and drill cutting weight has good agreement and one formulation is derived in this case.     

钢筋与混凝土界面的腐蚀疲劳

腐蚀疲劳是钢筋混凝土结构破坏的主要因素之一。基于剪切筒模型及文中选用的实验加载方式 ,引入界面摩擦系数衰减表达式 ,同时考虑力学因素———应力比R和加载频率 f的影响 ,研究了钢筋与混凝土界面在拉 -拉循环荷载作用下腐蚀疲劳裂纹扩展情况。通过对Paris公式的修正 ,借助能量耗散率给出腐蚀疲劳数学模型 ,得到了界面腐蚀疲劳脱粘速率 ,脱粘长度与循环荷载作用次数的关系。最后与无腐蚀情况的结果进行比较 ,分析了影响界面腐蚀疲劳 (CF)裂纹扩展速率的因素 ,如应力比 ,加载频率。     

Technology use and psychosocial factors in the self-reporting of musculoskeletal disorder symptoms in call center workers

PROBLEM: Evidence exists to suggest that working with computer terminals and keyboards is associated with the development and exacerbation of a range of musculoskeletal disorders (MSDs). Research has suggested that psychosocial factors may be of more importance than computer use factors in the development of MSDs in the computing workplace. METHOD: A questionnaire-based study was undertaken to test this hypothesis in a sample of 67 call center workers. A Spearman's rho was performed on the data, as well as chi(2) tests on the individual variables. RESULTS: It was found that overall, computer use factors were significantly associated with self-reporting of MSD symptoms, whereas psychosocial factors had no such association. However, certain individual psychosocial factors emerged as having a significant association with MSD symptoms. DISCUSSION: The findings suggest that specific areas of call center work have associations with poor worker health and possible long-term MSD problems. These areas appear to be workload and particular management-worker relations, rather than computer use. SUMMARY: This suggests that the relationship among computer use, psychosocial factors, and MSD is a complex area that would benefit from more research. IMPACT ON INDUSTRY: This study raises issues about the importance of psychosocial and organizational factors and employee welfare in the call center working environment.     

Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization

A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene (BaP) was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as Biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons (PAHs) (such as phenanthrene, pyrene and fluoranthene) as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry.