纳米氧化锌颗粒物通过氧化应激和离子通道失调诱导大鼠气管上皮细胞凋亡的机理

纳米氧化锌具有广泛的工业用途,其生态安全性受到广泛关注,针对纳米氧化锌诱导的呼吸道细胞毒性及其作用机理研究尚不广泛.本研究分别采用不同浓度和粒径(30 nm和90 nm)的氧化锌颗粒物处理大鼠气管上皮细胞(rat tracheal epithelial cells,RTE cells),暴露时间为12 h,通过检测细胞内锌元素含量,细胞增殖抑制率,细胞凋亡率,凋亡相关caspsae 3基因与蛋白相对表达量,细胞内金属硫蛋白活性,ROS和MDA含量、细胞内Ca~(2+)-ATP酶和Na~+/K~+-ATP酶活性来分析纳米氧化锌诱导细胞毒效应机理.在90 nm纳米氧化锌高浓度暴露时,其细胞内锌元素浓度为0.845μg·L~(-1),约为低浓度暴露组的4.7倍,是30 nm低浓度暴露组的9倍;纳米颗粒物诱导的细胞增殖和凋亡毒效应具有剂量和尺寸依赖效应;30 nm处理组的pro-caspase 3和cleaved-caspase 3蛋白表达量均高于90 nm暴露组;暴露浓度为10 mg·L~(-1)的90 nm处理组的金属硫蛋白增加量为0.533μg·L~(-1),增幅达到46%;不同粒径氧化锌颗粒物处理后,细胞内ROS和MDA含量显著上升,且30 nm处理组结果均高于90 nm处理组;纳米氧化锌颗粒物暴露诱导细胞Ca~(2+)-ATP酶和Na~+/K~+-ATP酶活性显著下降,30 nm氧化锌颗粒物暴露组,其Na~+/K~+-ATP酶活性分别是对照组的1.8倍和3.5倍.纳米氧化锌颗粒物进入RTE细胞,通过干扰锌在细胞内代谢,诱导细胞内ROS和MDA水平升高,产生氧化应激,进而诱导细胞凋亡是导致纳米氧化锌产生细胞毒性的主要原因之一.纳米氧化锌会导致细胞内Ca~(2+)-ATPase和Na~+/K~+-ATPase活性下降,离子通道失调,破坏细胞内离子平衡,进一步造成细胞凋亡.     

Numerical model of flow and pollutant transportation in non-orthogonal curvilinear coordinates

The planar 2D k-ε double equations‘ turbulence model was adopted and transformed into non-orthogonal curvilinear coordinates. The concentration convection-diffusion was introduced to planar 2D SIMPLEC algorithm of flow in non-orthogonal curvilinear coordinates. The numerical model of pollutant transportation in non-orthogonal curvilinear coordinates was constructed. The model was applied to simulate the flow and pollutant concentration fields. In the testing concentration field, two optimal operations of contamination discharging both along bank and in the centerline at the first bend of the meandering channel were adopted. Comparison with available data showed the model developed was successful, was valuable to engineering application.     

Integrating channel design and assessment methods based on sediment transport capacity in gravel bed streams

Natural channel design (NCD) and analytical channel design (ACD) are two competing approaches to stable channel design that share fundamental similarities in accounting for sediment transport processes with designs based on hybrid fluvial geomorphology and hydraulic engineering methods. In this paper, we highlight the linkage between ACD's capacity/supply ratio (CSR) and NCD's sediment capacity models (FLOWSED/POWERSED), illustrating how ACD and NCD have reached a point of convergent evolution within the stream restoration toolbox. We modified an existing CSR analytical spreadsheet tool which enabled us to predict relative channel stability using both conventional bed load transport equations and regional sediment regression curves. The stable channel design solutions based on measured data most closely matched the Parker (ACD) and/or Pagosa good/fair (NCD) relationships, which also showed the greatest CSR sensitivity in response to channel alterations. We found that CSR differences among the transport relationships became more extreme the further the design width deviated from the supply reach, suggesting that a stable upstream supply reach may serve as the best design analog. With this paper, we take a step toward resolving lingering controversy in the field of stream restoration, advancing the science and practice by reconciling key differences between ACD and NCD in the context of reach scale morphodynamics.     

城市河道再生水特征水质因子空间变异机制分析

受城市污水处理厂处理工艺的限制和水源来源复杂等因素限制,再生水中富含的无机氮和痕量持久性有机物在河湖补给过程中通过侧向渗漏或生物累积的方式造成潜在人体健康风险.探究再生水补给河道不同类型再生水特征水质因子空间变异规律及其成因对于河湖管理具有重要意义.以北运河京津冀段为研究区域,借助于聚类、判别、主成分和方差分解等方法探...     

机体细胞镉摄入离子转运通道研究进展

镉是人体非必需金属离子,长期镉暴露易引发镉中毒。机体内没有负责镉转运的特定载体,镉可通过必需金属离子转运载体进入机体细胞。机体内能够转运镉的载体有多种,主要包括铁的转运载体二价金属离子转运蛋白1(DMT1)、钙离子通道(电压门控钙通道(VGCC)、瞬时感受器电位(TRP)和钙库调控的钙通道(SOC))以及锌铁调控蛋白ZIP家族中的ZIP8和ZIP14等,且不同的机体细胞镉吸收所需转运载体不同。转运载体对镉离子的转运符合米氏方程,不同载体调节镉吸收的米氏常数Km值不同。机体细胞镉的吸收是个复杂的过程,通常存在着多种转运载体的交互作用,机体细胞可根据环境变化而选择镉的转运载体。对镉的生理毒性,以及细胞镉吸收常用的转运载体类型加以阐述,并分析了不同机体细胞镉吸收的可能转运载体,以期为后续探究机体细胞镉吸收具体分子机制提供理论指导。     

气固两相流下球阀磨损特性研究

旋塞球阀是钻柱内防喷系统中的关键设备,在气固两相流下球阀易受磨损而失效,并造成严重的井喷事故。为此,将计算流体动力学理论与冲蚀磨损理论相结合,运用FLUENT软件对球阀壁面在气固两相流下的磨损分布情况进行研究,并进一步分析了球阀结构参数对于球阀壁面磨损的影响规律。结果表明:当气固两相流流经球阀时,固体颗粒会与气流分离,并在壁面上产生三处磨损集中区;随着球阀开度的减小,球阀壁面磨损量会急剧增大,且阀球内通道壁面上的磨损集中区由块状逐步转化为带状,而球阀出口处的磨损集中区则会逐渐向下移动;球阀流道直径的减小也会使得壁面磨损量增加,但磨损集中区的分布基本不变。研究结果可为进一步优化球阀流道结构以减轻其壁面磨损提供理论依据。     

Modeling Monthly Fluctuations in Submersion Area of a Dammed River Reservoir: A Case Study1

Lu, Haorong, S. Samuel Li, and Jinsong Guo, 2012. Modeling Monthly Fluctuations in Submersion Area of a Dammed River Reservoir: A Case Study. Journal of the American Water Resources Association (JAWRA) 1‐13. DOI: 10.1111/jawr.12003 Abstract: Fluctuations in water submersion of the Three Gorges Reservoir in China have not been explored in spite of their important implications for shoreline erosion and other undesirable consequences. This article aims to quantify the monthly fluctuations in response to changing hydraulic parameters and regional climatic factors. Flow velocity and water levels distributed along the 609‐km long dammed river reservoir are calculated with a one‐dimensional hydrodynamics model. Evaporation of water from the surface of the reservoir is determined using mass transfer‐based methods. Calculated flow velocities and water levels compare well with field data. We show that the water surface slope decreases with rising water level at the dam, and decreases to almost zero during the winter months of water storage when the downstream water level reaches the normal pool level. The submersion area varies between 830 and 1,070 km² over the year or over 20% of the reservoir zone will experience the annual cycle of dry land and partial or complete submersion. These fluctuations are of relevance to shoreline management and to the prevention and restoration of shoreline erosion. Evaporation is estimated to fluctuate between 1,240 and 26,110 tons of water per month per kilometer length of reservoir channel; this can possibly affect the hydrological budget of the reservoir region. The simple methodologies discussed in this article can easily be applied to other dammed river reservoirs for submersion estimates.     

基于G1-VPRS-MIE多层次灰色理论的引水明渠运行风险评价

引水明渠的安全运行风险评价对减少和预防其在输水运行过程中的灾害发生、提高其在运营期间的社会经济效益具有重要的意义。为了对引水明渠在运行期间的风险进行合理赋权和评价,通过科学合理的方法构建了多指标参数的明渠运行风险影响因素指标体系,包括主体结构风险、自然风险、水污染风险、组织管理风险和运行调度风险5个风险因子子系统及20个二级指标在内的评价指标体系;运用G1和VPRS法分别确定各指标的主观权重和客观权重,结合MIE理论优化指标权重;根据明渠工程的运行特点,选取多层次灰色理论计算引水明渠运行风险值,并根据相关规范和安全风险评价实践确定风险评价等级。将该模型应用于引大入秦工程中,结果表明:采用序关系分析法(G1法)和变精度粗糙集理论(VPRS)并结合最小信息熵原理(MIE)的权重确定方法(G1-VPRS-MIE),不但避免了层次分析法过度依赖专家的主观经验、克服了粗糙集理论在边界刻画的局限性,而且弥补了主观和客观方法相结合的不足;对于指标权重的确定更加客观合理,既能满足所得权重的客观性,也能保证评估结果具有一定的解释性,同时能够减少单一权重计算的偏差;多层次灰色评价模型对于引水明渠安全运行风险等级的确定较为有效,并得出其风险等级为中等偏高,与实际情况一致。研究成果为引水明渠工程安全运行提供了一定的参考,同时可为类似气候环境的引水明渠区域规划、设计、施工提供有效的借鉴。     

我国七大流域河道生态用水现状评价

从河流生态用水的基本属性出发,提出了河道生态用水"量"与"质"的评价方法,其中量的评价方法包括水资源利用率法、实测径流法、消耗系数等3种方法,质的评价是基于污径比指标进行评价,并确定了"量"与"质"的评价标准。以我国松花江、辽河、海河、黄河、淮河、长江、珠江等7个流域为例,采用上述方法进行实证分析,结果表明,如果单纯从量的角度进行评价,仅海河、黄河流域存在河道生态用水短缺现象,其它流域总体上为基本不短缺。但是如果对生态用水的质进行评价,只有松花江、长江和珠江的水质可以满足生态用水的要求,其它流域均在较差等级以下。结果表明只有从量和质两个方面进行评价,才能够揭示出我国生态用水的水量性或者水质性短缺的现实状况。     

南京市内河道沉积物中重金属元素形态及Pb稳定同位素组成

采集南京市居住和商业区内的河道沉积物,研究重金属元素的形态分布及Pb稳定同位素组成,探讨城市河道沉积物中重金属的迁移潜力和推断污染特征. 沉积物中重金属改良的BCR三步顺序提取研究表明,沉积物中Cd和Zn主要分布于酸溶态;Pb和Cu主要分布于可氧化态;Ni和Cr主要分布于残渣态. Cd和Zn的环境效应最值得关注,而Ni和Cr环境影响最小. 沉积物中m(208Pb)/m(206Pb)和m(206Pb)/m(207Pb)分别为2.102 4～2.123 9和1.159 3～1.184 5. 居住和商业区内的河道沉积物Pb稳定同位素组成相近,具有相似污染源. 居住和商业区内沉积物的Pb稳定同位素比率与文献中南京大气、城市土壤Pb稳定比率相似,因而河道沉积物重金属富集与城市重金属污染密切相关.