An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

华南玄武岩红土化过程中微量元素地球化学

广东、海南的玄武岩．在强烈的红土化过程中．元素在红土剖面中发生迁移和残留、贫化和富集。根据红土化过程中元素的地球化学性状，将元素划分为活泼元素和不活泼元素两大类。并首次采用了元素它集因子这个概念来探讨红土化过程中微量元素的地球化学行为。最后讨论了红土化过程小金的表生富集作用。     

Prenatal investigation of a 45,X/46,X,r(?) karyotype in amniocytes using fluorescence in situ hybridization with an X-centromeric probe

The karyotype of cultured amniotic fluid cells obtained on the indication of advanced maternal age was shown to be a mosaic 45,X/46,X,r(?). The small size and banding pattern made it difficult to determine whether the ring was derived from and X or a Y chromosome, or even from an autosome. By using an X-centromeric probe and fluorescence in situ hybridization (FISH), we demonstrated the ring to have an X centromere. Thus, a more complete genetic counselling was possible. This confirms the usefulness of FISH in identifying and characterizing this and other chromosome rearrangements in prenatal diagnosis.     

中国东南大陆中生代火山岩带的火山岩相类型

本文根据岩浆作用的表现形式、火山喷发方式、搬运方式、堆积环境、成岩过程以及距火山口远近等因素，将岩浆作用产物的岩相类型分为侵入岩相、侵出岩相、火山岩相等三大类十二种岩相类型．并详细阐述了区内火山岩相类型及其时间分布特征。     

正己烷萃取—此外分光光度法测定矿井水中微量油

在进行矿井水中油类分析时，用正己烷作为萃取剂，以工作曲线代替标准曲线，从而使油平均回收率增加到96％以上。并在正己烷用量、水样预处理等测试参数选择上做了条件试验。     

唤起“求知欲”，治疗“厌学症”

本文分析了求知欲的由来及消退原因，“厌学症”的形成及治疗方案，试图唤起学生的求知欲，治疗他们的“厌学症”。     

Atmospheric particulate pollution of Lanzhou using magnetic measurements

１　ＩｎｔｒｏｄｕｃｔｉｏｎＡｔｍｏｓｐｈｅｒｉｃｐａｒｔｉｃｕｌａｔｅｉｓｏｎｅｏｆｔｈｅｍａｉｎａｔｍｏｓｐｈｅｒｉｃｐｏｌｌｕｔａｎｔｓｉｎＣｈｉｎａ．Ｉｔｍａｙｂｅｈａｒｍｆｕｌｉｔｓｅｌｆａｎｄｃａｎａｃｔａｓａｃａｒｒｉｅｒｏｆｔｏｘｉｃｍａｔｔｅｒｓｗｈｉｃｈｍａｙｈａｖｅｒｅｍａｒｋａｂｌｅｉｍｐａｃｔｓｏｎｈｕｍａｎｈｅａｌｔｈａｎｄａｔｍｏｓｐｈｅｒｉｃｅｎｖｉｒｏｎｍｅｎｔｑｕａｌｉｔｙ．Ｃｏｍｂｕｓｔｉｏｎｏｆｃｏａｌ，ｗｈｉｃｈｓｔｉｌｌｓｅｒｖｅｓａｓｐｒｉｍａｒｙｅ…     

低浓度渣油-水系乳化液的抑尘性能现场试验研究

本文介绍了作者用渣油－水系乳化液做路面抑尘剂在某矿露天坑３种不同类型路面进行的大规模工业性试验。现场测定结果表明：这种路面抑尘剂可以获得比较理想的防尘效果，长期使用该乳化液可取得显著的综合经济效益。     

生态农业建设中的水资源生命周期及系统功能响应

论文从生命周期角度，对原先自然环境恶劣、通过生态农业建设取得了良好效果的3个生态农业县的水资源利用进行了全过程研究。结果表明，水资源生命周期过程的进展情况受多种因素影响。目前，各生态农业县水资源生命周期过程仍处在发育的初级阶段。这一过程的完整性与系统生态、经济和社会功能的提高成正比。     

长江口岛屿湿地的底栖动物资源研究

调查了长江口3个岛屿湿地的底栖动物资源及其生态特征和环境功能。经鉴定底栖无脊椎动物有41种,优势种为河蚬(Corbiculafluminea)、焦河蓝蛤(Potamocorbulaustulata)、缢蛏(Sinonovaculaconstricta)、麂眼螺(Rissoinasp.)、光滑狭口螺(Stenothyraglabra)、谭氏泥蟹(Ilyrplaxdeschampsi)、无齿相手蟹(Sesarmadenaani)和天津厚蟹(Helicetridenstientsinensis)。讨论了底栖动物资源的主要生态学特征及其环境功能,提出了对底栖动物资源合理利用与保护的建议。