酵母双杂交技术构建重组人雌激素受体基因酵母

应用酵母双杂交技术构建重组人雌激素受体基因酵母,用以检测类/抗雌激素化合物和环境样品的类/抗雌激素活性.采用多聚酶链反应(PCR)法扩增人雌激素受体(hER)基因,构建诱饵质粒pGBKT7-ER LBD;分别提取并纯化两种含有ER共激活因子基因的靶质粒pGAD424-GRIP1和pGAD424-SRC1;诱饵质粒和靶质粒同时转染酵母细胞Y187,于营养缺陷型培养基(SD/-Trp/-Leu)上筛选阳性菌落,分别构建两种ER双杂交酵母ER+GRIP1和ER+SRC1.考察双杂交酵母与不同激素:17β-雌二醇(E2)、二氢睾酮(DHT)、孕酮(PG)以及三碘甲状腺原氨酸(T3)的结合情况,并考察了雌激素受体拮抗剂4-羟基他莫昔芬(4-OHT)与酵母的相互作用.结果表明:ER+GRIP1和ER+SRC1酵母均能够专一性的和E2结合,并存在显著的剂量-效应关系,E2对ER+GRIP1和ER+SRC1酵母β-半乳糖苷酶活性诱导的EC50值分别为7.3×10-11mol·L-1和1.5×10-10mol·L-1,其中ER+GRIP1酵母细胞诱导产生的酶活性值明显高于ER+SRC1酵母细胞.4-OHT能够抑制E2诱导ER+GRIP1酵母细胞产生的酶活性,并存在显著的剂量-效应关系,IC50值为1.0×10-7mol·L-1.表明重组人雌激素受体基因酵母可以用于检测化合物和环境样品的类/抗雌激素活性.     

Coupling urban 3-D information and circuit theory to advance the development of urban ecological networks

Ongoing, rapid urban growth accompanied by habitat fragmentation and loss challenges biodiversity conservation and leads to decreases in ecosystem services. Application of the concept of ecological networks in the preservation and restoration of connections among isolated patches of natural areas is a powerful conservation strategy. However, previous approaches often failed to objectively consider the impacts of complex 3-D city environments on ecological niches. We used airborne lidar-derived information on the 3-D structure of the built environment and vegetation and detailed land use and cover data to characterize habitat quality, niche diversity, and human disturbance and to predict habitat connectivity among 38 identified habitat core areas (HCAs) in Nanjing, China. We used circuit theory and Linkage Mapper to create a landscape resistance layer, simulate habitat connectivity, and identify and prioritize important corridors. We mapped 64 links by using current flow centrality to evaluate each HCA's contribution and the links that facilitate intact connectivity. Values were highest for HCA links located in the west, south, and northeast of the study area, where natural forests with complex 3-D structures predominate. Two smaller HCA areas had high centrality scores relative to their extents, which means they could act as important stepping stones in connectivity planning. The mapped pinch-point regions had narrow and fragile links among the HCAs, suggesting they require special protection. The barriers with the highest impact scores were mainly located at the HCA connections to Purple Mountain and, based on these high scores, are more likely to indicate important locations that can be restored to improve potential connections. Our novel framework allowed us to sufficiently convey spatially explicit information to identify targets for habitat restoration and potential pathways for species movement and dispersal. Such information is critical for assessing existing or potential habitats and corridors and developing strategic plans to balance habitat conservation and other land uses based on scientifically informed connectivity planning and implementation.     

特许物权变动中寻租行为的衍生路径及抑制

特许物权是自然资源用益物权。特许物权变动是指特许物权的设立、变更和终止行为。自然资源的稀缺性特征是为其变动过程被政府管制的重要原因,我国法律即采取特许物权变动行政许可制度,是国家为减少自然资源开发中的外部性问题而对私人权益实施的强制性干预。特许物权变动行政许可制度弱化特许物权人的最大化收益,产生了价格不菲的经济租。部分特许物权人为了谋求自身利益的最大化,会做出各种努力旨在影响特许物权变动中行政许可的向度与强度。一旦寻租行为得逞,自然资源的法定变动路径将备受扭曲,社会总体福利下降。寻租行为在特许物权变动中的发生类型呈现出独占型寻租和竞争型寻租两种,但其有着共同的衍生路径。追踪寻租行为的发生脉路,可以洞察到自然资源特许物权变动中寻租衍生的特殊动因。可以通过合理化特许物权结构、规范特许物权变动秩序和强化行政许可权力制衡力度等措施来对寻租的动因加以有效抑制。     

Current Practice and Future Prospects for Social Data in Coastal and Ocean Planning

Coastal and ocean planning comprises a broad field of practice. The goals, political processes, and approaches applied to planning initiatives may vary widely. However, all planning processes ultimately require adequate information on both the biophysical and social attributes of a planning region. In coastal and ocean planning practice, there are well‐established methods to assess biophysical attributes; however, less is understood about the role and assessment of social data. We conducted the first global assessment of the incorporation of social data in coastal and ocean planning. We drew on a comprehensive review of planning initiatives and a survey of coastal and ocean practitioners. There was significantly more incorporation of social data in multiuse versus conservation‐oriented planning. Practitioners engaged a wide range of social data, including governance, economic, and cultural attributes of planning regions and human impacts data. Less attention was given to ecosystem services and social–ecological linkages, both of which could improve coastal and ocean planning practice. Although practitioners recognize the value of social data, little funding is devoted to its collection and incorporation in plans. Increased capacity and sophistication in acquiring critical social and ecological data for planning is necessary to develop plans for more resilient coastal and ocean ecosystems and communities. We suggest that improving social data monitoring, and in particular spatial social data, to complement biophysical data, is necessary for providing holistic information for decision‐support tools and other methods. Moving beyond people as impacts to people as beneficiaries, through ecosystem services assessments, holds much potential to better incorporate the tenets of ecosystem‐based management into coastal and ocean planning by providing targets for linked biodiversity conservation and human welfare outcomes. La Práctica Actual y los Prospectos Futuros para los Datos Sociales en la Planeación Costera y Oceánica     

Combining culturomic sources to uncover trends in popularity and seasonal interest in plants

Culturomic tools enable the exploration of trends in human–nature interactions, although they entail inherent biases and necessitate careful validation. Furthermore, people may engage with nature across different culturomic data sets differently. We evaluated people's digital interest and engagement with plant species based on Wikipedia and Google data and explored the conservation implications of these temporal interest patterns. As a case study, we explored the digital footprints of the most popular plant species in Israel. We analyzed 4 years of daily page views from Hebrew Wikipedia and 10 years of daily Google search volume in Israel. We modeled popularity of plant species in these 2 data sets based on a suite of plant attributes. We further explored the seasonal trends of people's interest in each species. We found differences in how people interacted digitally with plants in Wikipedia and Google. Overall, in Google, searches for species that have utility to humans were more common, whereas in Wikipedia, plants that serve as cultural emblems received more attention. Furthermore, in Google, popular species attracted more attention over time, opposite to the trend in Wikipedia. In Google, interest in species with short bloom duration exhibited more pronounced seasonal patterns, whereas in Wikipedia, seasonality of interest increased as bloom duration increased. Together, our results suggest that people's digital interactions with nature may be inherently different depending on the sources explored, which may affect use of this information for conservation. Although culturomics holds much promise, better understanding of its underpinnings is important when translating insights into conservation actions.     

Designing individualisation of eco information: a conceptual framework and design toolkit

Eco-labelling is the practice of eco information provision that most directly addresses consumer behaviour. Nowadays, consumers are facing difficulties in perceiving and understanding existing eco labels. In previous work, we proposed the conceptual framework of eco information individualisation which tailors eco labels according to the specific needs of individual users using contextual technologies. This paper extends the conceptual framework by introducing a more structured way of considering the personal data and product data requirements, and reports the development of a design toolkit that aims to support designers in the designing of individualised eco information. A design workshop was carried out to introduce the concept to designers, and evaluate the usability and usefulness of the toolkit. Positive responses were received. The design outputs generated from the workshop were considered largely feasible and have the potential to be developed into digital prototypes. These indicate that it is possible for designers to learn to design eco information individualisation in a short time. This paper is a step towards a greater understanding of designing individualised eco information.     

1985年以来江苏省土地利用变化对人类活动程度的响应

利用1985、1995、2005和2008年4期遥感影像解译获取的土地利用变化数据,从土地利用变化速度、转移方向和土地利用程度方面,借助GIS空间分析和图谱分析技术及Ucinet社会网络分析软件,全面定量分析江苏省1985年以来人类活动作用下土地利用时空变化规律。研究表明:(1)1985~1995、1995~2005、2005~2008年3个时段江苏省的土地利用综合动态分别为12.95%、17.17%、15.03%,反应了人类活动对江苏省土地利用变化的影响程度呈现出"缓慢影响—剧烈影响—影响减缓"的倒U型曲线规律;(2)1985年以来,耕地、建设用地和水域之间的空间转移是江苏省土地利用转移的关键,也是人类影响土地利用最主要的表现形式,3种地类的相互转移占所有土地转移流的80%以上,其中耕地转为建设用地所占比例最大,主要分布苏锡常及南京4市的市辖区,以及昆山、江阴等经济发达的县市及苏北的宿迁市、淮安市、徐州市;(3)1985年以来,追求净产出(利润)的增长是土地利用变化的内在动力,导致建设用地增加及耕地减少,使得全省土地利用程度整体水平不断升高;受可驱动的有效资本量控制,经济发达的苏南地区一直是人类活动影响土地利用最为剧烈的区域,而苏北地区随着经济实力的不断提升土地利用受人类活动影响的程度也在逐渐增强。     

西南地区陆地植被生态系统NPP时空演变及驱动力分析

研究西南地区陆地植被生态系统净初级生产力(NPP)的时空演变特征及其驱动力,对区域生态环境保护具有重要的现实意义.利用2000～2021年MODIS NPP、1999～2021年基于站点的气象数据和2000～2020年土地利用类型等数据,结合主成分分析、残差分析、Theil-Sen Median趋势分析和偏相关分析等方法,研究西南地区陆地植被生态系统NPP时空演变及其对驱动力的响应特征.结果表明,时间上,2000～2021年西南地区植被NPP呈波动上升趋势,速率为3.54g·(m²·a)^-1.气候变化和人类活动影响下,农田、草地和森林生态系统NPP均呈上升趋势,但农田生态系统NPP的上升趋势最为显著.空间上,西南地区植被NPP呈上升趋势的面积占比为89.06%,显著上升和极显著上升的区域主要分布在广西南部、四川东部、重庆西部,以及云南和贵州交界处.气候变化和人类活动对西南地区植被生长具有双重影响,气候变化和人类活动影响下农田生态系统NPP呈上升趋势的面积占比均高于草地和森林生态系统.西南地区植被NPP与各气象因子的相关性呈明显地域差异.区域尺...     

气候变化和人类活动对我国典型草原区植被恢复的影响

草原区作为影响我国陆地生态系统碳水循环和生态安全的重点区域,对气候变化和人类活动极为敏感.然而,有关气候变化和人类活动对该区域植被恢复相对贡献的认识尚存分歧.以生态系统净初级生产力(NPP)为评价指标,通过对比MODIS观测的实际NPP和基于Thornthwaite Memorial模型估算的潜在NPP的趋势差异,量化了2000～2020年气候变化和人类活动对我国典型草原区(北方温性草原区和青藏高原高寒草原区)植被恢复的相对作用.结果表明,研究区内93%的草地植被呈恢复趋势,NPP平均增加速率达(以C计)2.12 g·(m²·a)^-1(P<0.01),其中,近一半植被恢复区受气候变化和人为活动共同控制,约36%和10%植被恢复区分别受气候变化和人类活动的独立控制;此外,不同草地类型气候变化主导植被恢复的面积占比差异大,主要表现为高寒草地明显高于温性草地,气候条件越干旱,气候主导面积占比越大.人类活动不是北方温性草原区和青藏高原高寒草原区植被恢复的主要原因,但在气候条件恶化地区,人类活动可降低甚至抵消气候变化对植被的负面影响.未来需加强长...     

气候变化背景下人类活动对承德接坝区植被净初级生产力的影响

承德接坝区位于农牧过渡区,对气候变化和人类活动极为敏感.以植被净初级生产力(NPP)作为评价指标,基于Thornthwaite Memorial模型计算潜在NPP和MODIS NPP遥感影像获取实际NPP数据,利用潜在NPP与实际NPP间的差值衡量人类活动作用下NPP的大小,运用Slope趋势和变异系数法分析实际NPP、潜在NPP和人类活动作用下NPP的变化趋势及稳定性分布,并采用相关系数法分析实际NPP与年降水量和年均气温间的相关性,最终量化气候变化和人类活动对该区域植被变化的影响.结果表明,潜在NPP自西北向东南递增,其变化趋势和稳定性均为自西向东递增.实际NPP与年降水量和年均气温呈正相关区域面积占比分别是99.87%和91.66%.该区域99.85%的植被得到改善且变化稳定,主要是由气候因素和人类活动共同主导(99.71%),而植被退化完全是由人为因素所导致(0.15%).